Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04965

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04955 and BWI76_RS04960 are separated by 50 nucleotidesBWI76_RS04960 and BWI76_RS04965 are separated by 107 nucleotidesBWI76_RS04965 and BWI76_RS04970 overlap by 4 nucleotidesBWI76_RS04970 and BWI76_RS04975 are separated by 121 nucleotides BWI76_RS04955: BWI76_RS04955 - hypoxanthine phosphoribosyltransferase, at 1,041,584 to 1,042,120 _RS04955 BWI76_RS04960: BWI76_RS04960 - carbonate dehydratase, at 1,042,171 to 1,042,833 _RS04960 BWI76_RS04965: BWI76_RS04965 - ABC transporter ATP-binding protein, at 1,042,941 to 1,043,867 _RS04965 BWI76_RS04970: BWI76_RS04970 - ABC transporter permease, at 1,043,864 to 1,044,634 _RS04970 BWI76_RS04975: BWI76_RS04975 - PTS fructose subfamily transporter subunit IIA, at 1,044,756 to 1,045,196 _RS04975 Position (kb) 1042 1043 1044Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1042.160 kb on - strandat 1042.160 kb on - strandat 1042.279 kb on + strand, within BWI76_RS04960at 1042.303 kb on - strandat 1042.491 kb on + strand, within BWI76_RS04960at 1042.585 kb on + strand, within BWI76_RS04960at 1042.624 kb on - strand, within BWI76_RS04960at 1042.785 kb on + strandat 1043.048 kb on + strand, within BWI76_RS04965at 1043.131 kb on - strand, within BWI76_RS04965at 1043.190 kb on - strand, within BWI76_RS04965at 1043.524 kb on + strand, within BWI76_RS04965at 1043.532 kb on - strand, within BWI76_RS04965at 1043.560 kb on + strand, within BWI76_RS04965at 1043.784 kb on - strandat 1044.143 kb on - strand, within BWI76_RS04970at 1044.297 kb on + strand, within BWI76_RS04970at 1044.305 kb on - strand, within BWI76_RS04970at 1044.654 kb on + strandat 1044.842 kb on + strand, within BWI76_RS04975

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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1,042,160 - -1.2
1,042,160 - -2.6
1,042,279 + BWI76_RS04960 0.16 -1.6
1,042,303 - -0.1
1,042,491 + BWI76_RS04960 0.48 +0.1
1,042,585 + BWI76_RS04960 0.62 +0.1
1,042,624 - BWI76_RS04960 0.68 -0.8
1,042,785 + -0.5
1,043,048 + BWI76_RS04965 0.12 +1.3
1,043,131 - BWI76_RS04965 0.20 +0.7
1,043,190 - BWI76_RS04965 0.27 -0.7
1,043,524 + BWI76_RS04965 0.63 +0.1
1,043,532 - BWI76_RS04965 0.64 +0.2
1,043,560 + BWI76_RS04965 0.67 +3.9
1,043,784 - +0.1
1,044,143 - BWI76_RS04970 0.36 -1.0
1,044,297 + BWI76_RS04970 0.56 +0.2
1,044,305 - BWI76_RS04970 0.57 -1.0
1,044,654 + -0.0
1,044,842 + BWI76_RS04975 0.20 +0.0

Or see this region's nucleotide sequence