Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01665

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01660 and BWI76_RS01665 are separated by 31 nucleotidesBWI76_RS01665 and BWI76_RS01670 are separated by 101 nucleotides BWI76_RS01660: BWI76_RS01660 - malate synthase A, at 336,918 to 338,519 _RS01660 BWI76_RS01665: BWI76_RS01665 - Isocitrate lyase (EC 4.1.3.1) (from data), at 338,551 to 339,855 _RS01665 BWI76_RS01670: BWI76_RS01670 - bifunctional isocitrate dehydrogenase kinase/phosphatase, at 339,957 to 341,699 _RS01670 Position (kb) 338 339 340Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 337.629 kb on - strand, within BWI76_RS01660at 337.650 kb on - strand, within BWI76_RS01660at 337.717 kb on + strand, within BWI76_RS01660at 337.826 kb on + strand, within BWI76_RS01660at 337.870 kb on - strand, within BWI76_RS01660at 338.149 kb on - strand, within BWI76_RS01660at 338.334 kb on + strand, within BWI76_RS01660at 338.609 kb on + strandat 339.333 kb on - strand, within BWI76_RS01665at 339.344 kb on + strand, within BWI76_RS01665at 339.487 kb on + strand, within BWI76_RS01665at 339.493 kb on + strand, within BWI76_RS01665at 339.493 kb on + strand, within BWI76_RS01665at 339.671 kb on - strand, within BWI76_RS01665at 339.781 kb on - strandat 339.811 kb on - strandat 339.820 kb on + strandat 339.820 kb on + strandat 339.820 kb on + strandat 339.975 kb on - strandat 340.081 kb on + strandat 340.113 kb on - strandat 340.198 kb on + strand, within BWI76_RS01670at 340.211 kb on - strand, within BWI76_RS01670at 340.285 kb on + strand, within BWI76_RS01670at 340.494 kb on - strand, within BWI76_RS01670at 340.701 kb on + strand, within BWI76_RS01670at 340.757 kb on + strand, within BWI76_RS01670at 340.785 kb on + strand, within BWI76_RS01670at 340.805 kb on + strand, within BWI76_RS01670

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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337,629 - BWI76_RS01660 0.44 -0.0
337,650 - BWI76_RS01660 0.46 -1.6
337,717 + BWI76_RS01660 0.50 +0.9
337,826 + BWI76_RS01660 0.57 -0.1
337,870 - BWI76_RS01660 0.59 -1.3
338,149 - BWI76_RS01660 0.77 -0.0
338,334 + BWI76_RS01660 0.88 +0.3
338,609 + -0.4
339,333 - BWI76_RS01665 0.60 +0.2
339,344 + BWI76_RS01665 0.61 +0.6
339,487 + BWI76_RS01665 0.72 +1.1
339,493 + BWI76_RS01665 0.72 +5.1
339,493 + BWI76_RS01665 0.72 +0.5
339,671 - BWI76_RS01665 0.86 -0.6
339,781 - -1.3
339,811 - +0.2
339,820 + -0.2
339,820 + +0.4
339,820 + -1.1
339,975 - -0.5
340,081 + +1.2
340,113 - -3.3
340,198 + BWI76_RS01670 0.14 -1.4
340,211 - BWI76_RS01670 0.15 +0.5
340,285 + BWI76_RS01670 0.19 -0.6
340,494 - BWI76_RS01670 0.31 +0.6
340,701 + BWI76_RS01670 0.43 -1.5
340,757 + BWI76_RS01670 0.46 +0.0
340,785 + BWI76_RS01670 0.48 +1.0
340,805 + BWI76_RS01670 0.49 +0.4

Or see this region's nucleotide sequence