Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26135 and BWI76_RS26140 are separated by 62 nucleotides BWI76_RS26140 and BWI76_RS26145 overlap by 4 nucleotides BWI76_RS26145 and BWI76_RS26150 are separated by 24 nucleotides
BWI76_RS26135: BWI76_RS26135 - Intracellular growth attenuator protein igaA, at 5,399,028 to 5,401,157
_RS26135
BWI76_RS26140: BWI76_RS26140 - GMP/IMP nucleotidase, at 5,401,220 to 5,401,903
_RS26140
BWI76_RS26145: BWI76_RS26145 - heat-shock protein Hsp15, at 5,401,900 to 5,402,301
_RS26145
BWI76_RS26150: BWI76_RS26150 - molecular chaperone Hsp33, at 5,402,326 to 5,403,204
_RS26150
Position (kb) 5401
5402
5403 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5401.280 kb on + strand at 5401.350 kb on + strand, within BWI76_RS26140 at 5401.432 kb on + strand, within BWI76_RS26140 at 5401.438 kb on - strand, within BWI76_RS26140 at 5401.440 kb on - strand, within BWI76_RS26140 at 5401.492 kb on - strand, within BWI76_RS26140 at 5401.636 kb on + strand, within BWI76_RS26140 at 5401.850 kb on - strand at 5401.886 kb on + strand at 5402.012 kb on + strand, within BWI76_RS26145 at 5402.020 kb on - strand, within BWI76_RS26145 at 5402.020 kb on - strand, within BWI76_RS26145 at 5402.111 kb on + strand, within BWI76_RS26145 at 5402.477 kb on + strand, within BWI76_RS26150 at 5402.696 kb on - strand, within BWI76_RS26150 at 5402.770 kb on - strand, within BWI76_RS26150 at 5402.838 kb on + strand, within BWI76_RS26150 at 5402.892 kb on - strand, within BWI76_RS26150
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 5,401,280 + -2.0 5,401,350 + BWI76_RS26140 0.19 -2.5 5,401,432 + BWI76_RS26140 0.31 -1.1 5,401,438 - BWI76_RS26140 0.32 +0.1 5,401,440 - BWI76_RS26140 0.32 +0.6 5,401,492 - BWI76_RS26140 0.40 +0.1 5,401,636 + BWI76_RS26140 0.61 -0.1 5,401,850 - -0.9 5,401,886 + -0.9 5,402,012 + BWI76_RS26145 0.28 +1.6 5,402,020 - BWI76_RS26145 0.30 -0.3 5,402,020 - BWI76_RS26145 0.30 -0.1 5,402,111 + BWI76_RS26145 0.52 +4.7 5,402,477 + BWI76_RS26150 0.17 +0.1 5,402,696 - BWI76_RS26150 0.42 +0.2 5,402,770 - BWI76_RS26150 0.51 -2.3 5,402,838 + BWI76_RS26150 0.58 -2.3 5,402,892 - BWI76_RS26150 0.64 -2.6
Or see this region's nucleotide sequence