Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25060 and BWI76_RS25065 are separated by 107 nucleotides BWI76_RS25065 and BWI76_RS25070 overlap by 42 nucleotides BWI76_RS25070 and BWI76_RS25075 are separated by 71 nucleotides BWI76_RS25075 and BWI76_RS25080 are separated by 147 nucleotides
BWI76_RS25060: BWI76_RS25060 - lipoprotein NlpI, at 5,217,454 to 5,218,338
_RS25060
BWI76_RS25065: BWI76_RS25065 - polyribonucleotide nucleotidyltransferase, at 5,218,446 to 5,220,584
_RS25065
BWI76_RS25070: BWI76_RS25070 - hypothetical protein, at 5,220,543 to 5,220,754
_RS25070
BWI76_RS25075: BWI76_RS25075 - 30S ribosomal protein S15, at 5,220,826 to 5,221,095
_RS25075
BWI76_RS25080: BWI76_RS25080 - tRNA pseudouridine(55) synthase, at 5,221,243 to 5,222,187
_RS25080
Position (kb) 5218
5219
5220
5221 Strain fitness (log2 ratio) -1
0
1
2 at 5217.685 kb on - strand, within BWI76_RS25060 at 5217.919 kb on - strand, within BWI76_RS25060 at 5218.150 kb on - strand, within BWI76_RS25060 at 5218.177 kb on - strand, within BWI76_RS25060 at 5218.197 kb on - strand, within BWI76_RS25060 at 5218.294 kb on + strand at 5219.314 kb on + strand, within BWI76_RS25065 at 5219.551 kb on + strand, within BWI76_RS25065 at 5219.825 kb on + strand, within BWI76_RS25065 at 5220.072 kb on - strand, within BWI76_RS25065 at 5220.091 kb on + strand, within BWI76_RS25065 at 5220.430 kb on + strand at 5220.472 kb on + strand at 5220.681 kb on - strand, within BWI76_RS25070 at 5220.709 kb on - strand at 5221.250 kb on + strand at 5221.407 kb on - strand, within BWI76_RS25080 at 5221.453 kb on + strand, within BWI76_RS25080 at 5221.520 kb on + strand, within BWI76_RS25080
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 5,217,685 - BWI76_RS25060 0.26 +1.3 5,217,919 - BWI76_RS25060 0.53 +0.9 5,218,150 - BWI76_RS25060 0.79 +2.0 5,218,177 - BWI76_RS25060 0.82 +2.5 5,218,197 - BWI76_RS25060 0.84 -0.2 5,218,294 + +1.2 5,219,314 + BWI76_RS25065 0.41 +1.6 5,219,551 + BWI76_RS25065 0.52 -0.7 5,219,825 + BWI76_RS25065 0.64 +2.4 5,220,072 - BWI76_RS25065 0.76 -1.2 5,220,091 + BWI76_RS25065 0.77 +1.2 5,220,430 + -0.4 5,220,472 + +2.4 5,220,681 - BWI76_RS25070 0.65 -1.0 5,220,709 - -0.0 5,221,250 + +0.7 5,221,407 - BWI76_RS25080 0.17 -0.7 5,221,453 + BWI76_RS25080 0.22 +1.0 5,221,520 + BWI76_RS25080 0.29 -0.8
Or see this region's nucleotide sequence