Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21910 and BWI76_RS21915 are separated by 74 nucleotides BWI76_RS21915 and BWI76_RS21920 are separated by 6 nucleotides BWI76_RS21920 and BWI76_RS21925 are separated by 19 nucleotides
BWI76_RS21910: BWI76_RS21910 - glyoxylate/hydroxypyruvate reductase A, at 4,578,260 to 4,579,192
_RS21910
BWI76_RS21915: BWI76_RS21915 - short-chain dehydrogenase/reductase SDR, at 4,579,267 to 4,580,061
_RS21915
BWI76_RS21920: BWI76_RS21920 - amino acid ABC transporter substrate-binding protein, at 4,580,068 to 4,580,284
_RS21920
BWI76_RS21925: BWI76_RS21925 - agmatinase, at 4,580,304 to 4,581,275
_RS21925
Position (kb) 4579
4580
4581 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 4578.296 kb on + strand at 4578.304 kb on - strand at 4578.342 kb on - strand at 4578.700 kb on + strand, within BWI76_RS21910 at 4578.843 kb on - strand, within BWI76_RS21910 at 4578.855 kb on - strand, within BWI76_RS21910 at 4579.229 kb on + strand at 4579.405 kb on + strand, within BWI76_RS21915 at 4579.405 kb on + strand, within BWI76_RS21915 at 4579.660 kb on + strand, within BWI76_RS21915 at 4579.683 kb on - strand, within BWI76_RS21915 at 4579.991 kb on + strand at 4580.000 kb on + strand at 4580.073 kb on + strand at 4580.143 kb on - strand, within BWI76_RS21920 at 4580.145 kb on - strand, within BWI76_RS21920 at 4580.152 kb on + strand, within BWI76_RS21920 at 4580.152 kb on + strand, within BWI76_RS21920 at 4580.595 kb on + strand, within BWI76_RS21925 at 4580.630 kb on + strand, within BWI76_RS21925 at 4580.673 kb on + strand, within BWI76_RS21925 at 4580.673 kb on - strand, within BWI76_RS21925 at 4580.998 kb on + strand, within BWI76_RS21925
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 4,578,296 + +0.5 4,578,304 - -2.3 4,578,342 - -0.2 4,578,700 + BWI76_RS21910 0.47 -0.2 4,578,843 - BWI76_RS21910 0.62 +0.1 4,578,855 - BWI76_RS21910 0.64 -0.2 4,579,229 + -0.2 4,579,405 + BWI76_RS21915 0.17 -0.8 4,579,405 + BWI76_RS21915 0.17 +5.1 4,579,660 + BWI76_RS21915 0.49 -2.4 4,579,683 - BWI76_RS21915 0.52 +0.1 4,579,991 + -0.1 4,580,000 + +1.3 4,580,073 + -0.4 4,580,143 - BWI76_RS21920 0.35 +1.3 4,580,145 - BWI76_RS21920 0.35 +1.1 4,580,152 + BWI76_RS21920 0.39 +1.9 4,580,152 + BWI76_RS21920 0.39 +0.4 4,580,595 + BWI76_RS21925 0.30 -1.2 4,580,630 + BWI76_RS21925 0.34 +0.6 4,580,673 + BWI76_RS21925 0.38 -0.1 4,580,673 - BWI76_RS21925 0.38 -2.5 4,580,998 + BWI76_RS21925 0.71 -0.0
Or see this region's nucleotide sequence