Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20525 and BWI76_RS20530 are separated by 130 nucleotides BWI76_RS20530 and BWI76_RS20535 are separated by 48 nucleotides BWI76_RS20535 and BWI76_RS20540 are separated by 348 nucleotides
BWI76_RS20525: BWI76_RS20525 - glyceraldehyde 3-phosphate reductase, at 4,299,367 to 4,300,359
_RS20525
BWI76_RS20530: BWI76_RS20530 - hypothetical protein, at 4,300,490 to 4,300,849
_RS20530
BWI76_RS20535: BWI76_RS20535 - manganese transporter MntH, at 4,300,898 to 4,302,133
_RS20535
BWI76_RS20540: BWI76_RS20540 - NupC/NupG family nucleoside CNT transporter, at 4,302,482 to 4,303,684
_RS20540
Position (kb) 4300
4301
4302
4303 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4299.979 kb on - strand, within BWI76_RS20525 at 4300.019 kb on + strand, within BWI76_RS20525 at 4300.207 kb on + strand, within BWI76_RS20525 at 4300.215 kb on - strand, within BWI76_RS20525 at 4300.215 kb on - strand, within BWI76_RS20525 at 4300.241 kb on + strand, within BWI76_RS20525 at 4300.275 kb on + strand at 4300.417 kb on - strand at 4300.434 kb on + strand at 4300.447 kb on + strand at 4300.488 kb on - strand at 4300.563 kb on + strand, within BWI76_RS20530 at 4300.704 kb on - strand, within BWI76_RS20530 at 4300.726 kb on + strand, within BWI76_RS20530 at 4301.402 kb on + strand, within BWI76_RS20535 at 4302.305 kb on - strand at 4302.307 kb on + strand at 4302.479 kb on + strand at 4302.479 kb on + strand at 4302.646 kb on - strand, within BWI76_RS20540 at 4302.737 kb on + strand, within BWI76_RS20540 at 4302.854 kb on - strand, within BWI76_RS20540 at 4302.889 kb on + strand, within BWI76_RS20540
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 4,299,979 - BWI76_RS20525 0.62 -0.1 4,300,019 + BWI76_RS20525 0.66 +0.7 4,300,207 + BWI76_RS20525 0.85 -0.9 4,300,215 - BWI76_RS20525 0.85 -0.9 4,300,215 - BWI76_RS20525 0.85 +1.5 4,300,241 + BWI76_RS20525 0.88 -3.2 4,300,275 + -1.4 4,300,417 - +2.1 4,300,434 + +0.1 4,300,447 + -1.5 4,300,488 - -2.4 4,300,563 + BWI76_RS20530 0.20 +0.9 4,300,704 - BWI76_RS20530 0.59 +0.7 4,300,726 + BWI76_RS20530 0.66 -0.1 4,301,402 + BWI76_RS20535 0.41 +0.0 4,302,305 - -1.4 4,302,307 + +0.3 4,302,479 + -0.2 4,302,479 + +0.5 4,302,646 - BWI76_RS20540 0.14 +0.2 4,302,737 + BWI76_RS20540 0.21 -1.0 4,302,854 - BWI76_RS20540 0.31 -1.5 4,302,889 + BWI76_RS20540 0.34 -1.1
Or see this region's nucleotide sequence