Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20525

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20520 and BWI76_RS20525 are separated by 176 nucleotidesBWI76_RS20525 and BWI76_RS20530 are separated by 130 nucleotidesBWI76_RS20530 and BWI76_RS20535 are separated by 48 nucleotides BWI76_RS20520: BWI76_RS20520 - indolepyruvate decarboxylase, at 4,297,529 to 4,299,190 _RS20520 BWI76_RS20525: BWI76_RS20525 - glyceraldehyde 3-phosphate reductase, at 4,299,367 to 4,300,359 _RS20525 BWI76_RS20530: BWI76_RS20530 - hypothetical protein, at 4,300,490 to 4,300,849 _RS20530 BWI76_RS20535: BWI76_RS20535 - manganese transporter MntH, at 4,300,898 to 4,302,133 _RS20535 Position (kb) 4299 4300 4301Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4298.629 kb on + strand, within BWI76_RS20520at 4298.672 kb on - strand, within BWI76_RS20520at 4298.885 kb on - strand, within BWI76_RS20520at 4298.905 kb on - strand, within BWI76_RS20520at 4298.914 kb on - strand, within BWI76_RS20520at 4299.045 kb on - strandat 4299.245 kb on + strandat 4299.253 kb on - strandat 4299.253 kb on - strandat 4299.253 kb on - strandat 4299.386 kb on - strandat 4299.756 kb on - strand, within BWI76_RS20525at 4299.825 kb on + strand, within BWI76_RS20525at 4299.979 kb on - strand, within BWI76_RS20525at 4300.019 kb on + strand, within BWI76_RS20525at 4300.207 kb on + strand, within BWI76_RS20525at 4300.215 kb on - strand, within BWI76_RS20525at 4300.215 kb on - strand, within BWI76_RS20525at 4300.241 kb on + strand, within BWI76_RS20525at 4300.275 kb on + strandat 4300.417 kb on - strandat 4300.434 kb on + strandat 4300.447 kb on + strandat 4300.488 kb on - strandat 4300.563 kb on + strand, within BWI76_RS20530at 4300.704 kb on - strand, within BWI76_RS20530at 4300.726 kb on + strand, within BWI76_RS20530

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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4,298,629 + BWI76_RS20520 0.66 +0.9
4,298,672 - BWI76_RS20520 0.69 +1.9
4,298,885 - BWI76_RS20520 0.82 -0.3
4,298,905 - BWI76_RS20520 0.83 -2.7
4,298,914 - BWI76_RS20520 0.83 -0.9
4,299,045 - +0.9
4,299,245 + -1.7
4,299,253 - -0.8
4,299,253 - -1.4
4,299,253 - +0.3
4,299,386 - +0.1
4,299,756 - BWI76_RS20525 0.39 +0.8
4,299,825 + BWI76_RS20525 0.46 -2.0
4,299,979 - BWI76_RS20525 0.62 -0.1
4,300,019 + BWI76_RS20525 0.66 +0.7
4,300,207 + BWI76_RS20525 0.85 -0.9
4,300,215 - BWI76_RS20525 0.85 -0.9
4,300,215 - BWI76_RS20525 0.85 +1.5
4,300,241 + BWI76_RS20525 0.88 -3.2
4,300,275 + -1.4
4,300,417 - +2.1
4,300,434 + +0.1
4,300,447 + -1.5
4,300,488 - -2.4
4,300,563 + BWI76_RS20530 0.20 +0.9
4,300,704 - BWI76_RS20530 0.59 +0.7
4,300,726 + BWI76_RS20530 0.66 -0.1

Or see this region's nucleotide sequence