Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20515 and BWI76_RS20520 overlap by 4 nucleotides BWI76_RS20520 and BWI76_RS20525 are separated by 176 nucleotides
BWI76_RS20515: BWI76_RS20515 - ion channel protein, at 4,296,297 to 4,297,532
_RS20515
BWI76_RS20520: BWI76_RS20520 - indolepyruvate decarboxylase, at 4,297,529 to 4,299,190
_RS20520
BWI76_RS20525: BWI76_RS20525 - glyceraldehyde 3-phosphate reductase, at 4,299,367 to 4,300,359
_RS20525
Position (kb) 4297
4298
4299
4300 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 4296.637 kb on + strand at 4296.645 kb on - strand, within BWI76_RS20515 at 4296.779 kb on - strand, within BWI76_RS20515 at 4296.955 kb on - strand, within BWI76_RS20515 at 4297.144 kb on - strand, within BWI76_RS20515 at 4297.176 kb on - strand, within BWI76_RS20515 at 4297.311 kb on - strand, within BWI76_RS20515 at 4297.557 kb on - strand at 4297.561 kb on + strand at 4297.626 kb on - strand at 4297.842 kb on - strand, within BWI76_RS20520 at 4297.905 kb on + strand, within BWI76_RS20520 at 4298.009 kb on - strand, within BWI76_RS20520 at 4298.265 kb on - strand, within BWI76_RS20520 at 4298.302 kb on + strand, within BWI76_RS20520 at 4298.302 kb on + strand, within BWI76_RS20520 at 4298.629 kb on + strand, within BWI76_RS20520 at 4298.672 kb on - strand, within BWI76_RS20520 at 4298.885 kb on - strand, within BWI76_RS20520 at 4298.905 kb on - strand, within BWI76_RS20520 at 4298.914 kb on - strand, within BWI76_RS20520 at 4299.045 kb on - strand at 4299.245 kb on + strand at 4299.253 kb on - strand at 4299.253 kb on - strand at 4299.253 kb on - strand at 4299.386 kb on - strand at 4299.756 kb on - strand, within BWI76_RS20525 at 4299.825 kb on + strand, within BWI76_RS20525 at 4299.979 kb on - strand, within BWI76_RS20525 at 4300.019 kb on + strand, within BWI76_RS20525
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 4,296,637 + +0.4 4,296,645 - BWI76_RS20515 0.28 +0.6 4,296,779 - BWI76_RS20515 0.39 +0.8 4,296,955 - BWI76_RS20515 0.53 -1.0 4,297,144 - BWI76_RS20515 0.69 -1.8 4,297,176 - BWI76_RS20515 0.71 +0.8 4,297,311 - BWI76_RS20515 0.82 +5.9 4,297,557 - -0.2 4,297,561 + -0.6 4,297,626 - +0.2 4,297,842 - BWI76_RS20520 0.19 -1.1 4,297,905 + BWI76_RS20520 0.23 -0.1 4,298,009 - BWI76_RS20520 0.29 -2.8 4,298,265 - BWI76_RS20520 0.44 -1.5 4,298,302 + BWI76_RS20520 0.47 -0.5 4,298,302 + BWI76_RS20520 0.47 -1.8 4,298,629 + BWI76_RS20520 0.66 +0.9 4,298,672 - BWI76_RS20520 0.69 +1.9 4,298,885 - BWI76_RS20520 0.82 -0.3 4,298,905 - BWI76_RS20520 0.83 -2.7 4,298,914 - BWI76_RS20520 0.83 -0.9 4,299,045 - +0.9 4,299,245 + -1.7 4,299,253 - -0.8 4,299,253 - -1.4 4,299,253 - +0.3 4,299,386 - +0.1 4,299,756 - BWI76_RS20525 0.39 +0.8 4,299,825 + BWI76_RS20525 0.46 -2.0 4,299,979 - BWI76_RS20525 0.62 -0.1 4,300,019 + BWI76_RS20525 0.66 +0.7
Or see this region's nucleotide sequence