Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20170 and BWI76_RS20175 overlap by 4 nucleotides BWI76_RS20175 and BWI76_RS20180 are separated by 2 nucleotides BWI76_RS20180 and BWI76_RS20185 are separated by 98 nucleotides BWI76_RS20185 and BWI76_RS20190 are separated by 15 nucleotides
BWI76_RS20170: BWI76_RS20170 - NADH-quinone oxidoreductase subunit F, at 4,227,130 to 4,228,467
_RS20170
BWI76_RS20175: BWI76_RS20175 - NADH-quinone oxidoreductase subunit E, at 4,228,464 to 4,228,964
_RS20175
BWI76_RS20180: BWI76_RS20180 - NADH-quinone oxidoreductase subunit C/D, at 4,228,967 to 4,230,763
_RS20180
BWI76_RS20185: BWI76_RS20185 - NADH-quinone oxidoreductase subunit B, at 4,230,862 to 4,231,536
_RS20185
BWI76_RS20190: BWI76_RS20190 - NADH-quinone oxidoreductase subunit A, at 4,231,552 to 4,231,998
_RS20190
Position (kb)
4228
4229
4230
4231 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4228.008 kb on - strand, within BWI76_RS20170 at 4228.295 kb on - strand, within BWI76_RS20170 at 4228.438 kb on + strand at 4228.521 kb on - strand, within BWI76_RS20175 at 4229.509 kb on - strand, within BWI76_RS20180 at 4229.533 kb on + strand, within BWI76_RS20180 at 4229.573 kb on - strand, within BWI76_RS20180 at 4229.753 kb on + strand, within BWI76_RS20180 at 4230.095 kb on + strand, within BWI76_RS20180 at 4230.096 kb on - strand, within BWI76_RS20180 at 4230.263 kb on - strand, within BWI76_RS20180 at 4230.307 kb on + strand, within BWI76_RS20180 at 4230.870 kb on + strand at 4231.087 kb on + strand, within BWI76_RS20185 at 4231.302 kb on - strand at 4231.557 kb on + strand at 4231.565 kb on - strand at 4231.591 kb on - strand at 4231.657 kb on - strand, within BWI76_RS20190
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 4,228,008 - BWI76_RS20170 0.66 -1.4 4,228,295 - BWI76_RS20170 0.87 -0.4 4,228,438 + -1.9 4,228,521 - BWI76_RS20175 0.11 -2.0 4,229,509 - BWI76_RS20180 0.30 -3.5 4,229,533 + BWI76_RS20180 0.31 -2.6 4,229,573 - BWI76_RS20180 0.34 -1.0 4,229,753 + BWI76_RS20180 0.44 -3.0 4,230,095 + BWI76_RS20180 0.63 +1.1 4,230,096 - BWI76_RS20180 0.63 -0.2 4,230,263 - BWI76_RS20180 0.72 -1.0 4,230,307 + BWI76_RS20180 0.75 -2.2 4,230,870 + -0.8 4,231,087 + BWI76_RS20185 0.33 +1.6 4,231,302 - +1.0 4,231,557 + -1.1 4,231,565 - +0.2 4,231,591 - -1.2 4,231,657 - BWI76_RS20190 0.23 -1.2
Or see this region's nucleotide sequence