Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18175 and BWI76_RS18180 overlap by 4 nucleotides BWI76_RS18180 and BWI76_RS18185 are separated by 53 nucleotides BWI76_RS18185 and BWI76_RS18190 are separated by 39 nucleotides BWI76_RS18190 and BWI76_RS18195 overlap by 4 nucleotides
BWI76_RS18175: BWI76_RS18175 - hydrolase, at 3,776,951 to 3,777,517
_RS18175
BWI76_RS18180: BWI76_RS18180 - hypothetical protein, at 3,777,514 to 3,778,332
_RS18180
BWI76_RS18185: BWI76_RS18185 - membrane protein, at 3,778,386 to 3,778,781
_RS18185
BWI76_RS18190: BWI76_RS18190 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 3,778,821 to 3,779,564
_RS18190
BWI76_RS18195: BWI76_RS18195 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 3,779,561 to 3,780,577
_RS18195
Position (kb) 3778
3779 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 3777.471 kb on + strand at 3777.489 kb on + strand at 3777.489 kb on + strand at 3777.583 kb on - strand at 3777.748 kb on + strand, within BWI76_RS18180 at 3777.799 kb on + strand, within BWI76_RS18180 at 3777.930 kb on + strand, within BWI76_RS18180 at 3777.977 kb on - strand, within BWI76_RS18180 at 3778.454 kb on + strand, within BWI76_RS18185 at 3778.478 kb on - strand, within BWI76_RS18185 at 3778.484 kb on - strand, within BWI76_RS18185 at 3778.603 kb on - strand, within BWI76_RS18185 at 3778.605 kb on + strand, within BWI76_RS18185 at 3778.649 kb on + strand, within BWI76_RS18185 at 3778.744 kb on + strand at 3778.752 kb on - strand at 3779.110 kb on - strand, within BWI76_RS18190 at 3779.150 kb on + strand, within BWI76_RS18190 at 3779.775 kb on + strand, within BWI76_RS18195
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 3,777,471 + -1.5 3,777,489 + +0.3 3,777,489 + -1.2 3,777,583 - -0.7 3,777,748 + BWI76_RS18180 0.29 +0.2 3,777,799 + BWI76_RS18180 0.35 -0.7 3,777,930 + BWI76_RS18180 0.51 -1.3 3,777,977 - BWI76_RS18180 0.57 -0.5 3,778,454 + BWI76_RS18185 0.17 -1.8 3,778,478 - BWI76_RS18185 0.23 -3.3 3,778,484 - BWI76_RS18185 0.25 +10.2 3,778,603 - BWI76_RS18185 0.55 -1.4 3,778,605 + BWI76_RS18185 0.55 -1.9 3,778,649 + BWI76_RS18185 0.66 +0.4 3,778,744 + -0.4 3,778,752 - -1.1 3,779,110 - BWI76_RS18190 0.39 -0.3 3,779,150 + BWI76_RS18190 0.44 -0.6 3,779,775 + BWI76_RS18195 0.21 -2.2
Or see this region's nucleotide sequence