Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06065 and BWI76_RS06070 are separated by 11 nucleotides BWI76_RS06070 and BWI76_RS06075 overlap by 4 nucleotides BWI76_RS06075 and BWI76_RS06080 overlap by 4 nucleotides
BWI76_RS06065: BWI76_RS06065 - taurine ABC transporter substrate-binding protein, at 1,274,898 to 1,275,860
_RS06065
BWI76_RS06070: BWI76_RS06070 - taurine transporter ATP-binding subunit, at 1,275,872 to 1,276,639
_RS06070
BWI76_RS06075: BWI76_RS06075 - taurine transporter subunit, at 1,276,636 to 1,277,463
_RS06075
BWI76_RS06080: BWI76_RS06080 - taurine dioxygenase, at 1,277,460 to 1,278,311
_RS06080
Position (kb) 1275
1276
1277 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1274.990 kb on - strand at 1275.225 kb on + strand, within BWI76_RS06065 at 1275.266 kb on + strand, within BWI76_RS06065 at 1275.302 kb on - strand, within BWI76_RS06065 at 1275.326 kb on - strand, within BWI76_RS06065 at 1275.355 kb on + strand, within BWI76_RS06065 at 1275.462 kb on + strand, within BWI76_RS06065 at 1275.507 kb on - strand, within BWI76_RS06065 at 1275.512 kb on + strand, within BWI76_RS06065 at 1275.767 kb on + strand at 1276.057 kb on - strand, within BWI76_RS06070 at 1276.071 kb on - strand, within BWI76_RS06070 at 1276.071 kb on - strand, within BWI76_RS06070 at 1276.143 kb on - strand, within BWI76_RS06070 at 1276.221 kb on + strand, within BWI76_RS06070 at 1276.606 kb on - strand at 1276.708 kb on + strand at 1276.735 kb on + strand, within BWI76_RS06075 at 1277.315 kb on + strand, within BWI76_RS06075
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 1,274,990 - +1.2 1,275,225 + BWI76_RS06065 0.34 -2.8 1,275,266 + BWI76_RS06065 0.38 +1.2 1,275,302 - BWI76_RS06065 0.42 +0.1 1,275,326 - BWI76_RS06065 0.44 -0.2 1,275,355 + BWI76_RS06065 0.47 +1.1 1,275,462 + BWI76_RS06065 0.59 -2.1 1,275,507 - BWI76_RS06065 0.63 +2.3 1,275,512 + BWI76_RS06065 0.64 -1.2 1,275,767 + -0.6 1,276,057 - BWI76_RS06070 0.24 +2.8 1,276,071 - BWI76_RS06070 0.26 +0.2 1,276,071 - BWI76_RS06070 0.26 +0.6 1,276,143 - BWI76_RS06070 0.35 +1.1 1,276,221 + BWI76_RS06070 0.45 +1.2 1,276,606 - -0.2 1,276,708 + +0.1 1,276,735 + BWI76_RS06075 0.12 -1.0 1,277,315 + BWI76_RS06075 0.82 +0.9
Or see this region's nucleotide sequence