Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03135 and BWI76_RS03140 are separated by 190 nucleotides BWI76_RS03140 and BWI76_RS03145 are separated by 233 nucleotides BWI76_RS03145 and BWI76_RS03150 are separated by 164 nucleotides
BWI76_RS03135: BWI76_RS03135 - sugar ABC transporter permease, at 646,110 to 646,982
_RS03135
BWI76_RS03140: BWI76_RS03140 - alcohol dehydrogenase, at 647,173 to 648,192
_RS03140
BWI76_RS03145: BWI76_RS03145 - tRNA-Leu, at 648,426 to 648,510
_RS03145
BWI76_RS03150: BWI76_RS03150 - site-specific recombinase, phage integrase family protein, at 648,675 to 649,937
_RS03150
Position (kb) 647
648
649 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 646.254 kb on - strand, within BWI76_RS03135 at 646.254 kb on - strand, within BWI76_RS03135 at 646.321 kb on + strand, within BWI76_RS03135 at 646.421 kb on + strand, within BWI76_RS03135 at 646.524 kb on - strand, within BWI76_RS03135 at 646.541 kb on + strand, within BWI76_RS03135 at 646.598 kb on + strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.639 kb on + strand, within BWI76_RS03135 at 646.647 kb on + strand, within BWI76_RS03135 at 646.768 kb on + strand, within BWI76_RS03135 at 646.768 kb on + strand, within BWI76_RS03135 at 646.881 kb on - strand, within BWI76_RS03135 at 646.887 kb on + strand, within BWI76_RS03135 at 647.206 kb on + strand at 647.260 kb on - strand at 647.274 kb on + strand at 647.378 kb on - strand, within BWI76_RS03140 at 647.610 kb on + strand, within BWI76_RS03140 at 647.632 kb on - strand, within BWI76_RS03140 at 647.891 kb on + strand, within BWI76_RS03140 at 647.899 kb on - strand, within BWI76_RS03140 at 647.945 kb on + strand, within BWI76_RS03140 at 647.954 kb on + strand, within BWI76_RS03140 at 647.954 kb on + strand, within BWI76_RS03140 at 647.992 kb on + strand, within BWI76_RS03140 at 647.992 kb on + strand, within BWI76_RS03140 at 648.090 kb on - strand, within BWI76_RS03140 at 648.195 kb on - strand at 648.217 kb on + strand at 648.237 kb on - strand at 648.241 kb on + strand at 648.285 kb on - strand at 648.288 kb on - strand at 648.309 kb on - strand at 648.685 kb on + strand at 648.691 kb on + strand at 648.695 kb on + strand at 648.703 kb on - strand at 648.710 kb on + strand at 648.841 kb on + strand, within BWI76_RS03150 at 648.867 kb on - strand, within BWI76_RS03150 at 648.869 kb on + strand, within BWI76_RS03150 at 648.877 kb on - strand, within BWI76_RS03150 at 648.877 kb on - strand, within BWI76_RS03150 at 648.906 kb on + strand, within BWI76_RS03150 at 648.906 kb on + strand, within BWI76_RS03150 at 648.913 kb on + strand, within BWI76_RS03150 at 648.921 kb on - strand, within BWI76_RS03150 at 648.963 kb on + strand, within BWI76_RS03150 at 649.056 kb on - strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.097 kb on + strand, within BWI76_RS03150 at 649.112 kb on + strand, within BWI76_RS03150 at 649.151 kb on + strand, within BWI76_RS03150 at 649.159 kb on - strand, within BWI76_RS03150
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 646,254 - BWI76_RS03135 0.16 +0.8 646,254 - BWI76_RS03135 0.16 +0.1 646,321 + BWI76_RS03135 0.24 -0.3 646,421 + BWI76_RS03135 0.36 -2.6 646,524 - BWI76_RS03135 0.47 -3.1 646,541 + BWI76_RS03135 0.49 -0.9 646,598 + BWI76_RS03135 0.56 -1.8 646,606 - BWI76_RS03135 0.57 -0.1 646,606 - BWI76_RS03135 0.57 -2.5 646,606 - BWI76_RS03135 0.57 -3.1 646,639 + BWI76_RS03135 0.61 -0.4 646,647 + BWI76_RS03135 0.62 +2.4 646,768 + BWI76_RS03135 0.75 -0.6 646,768 + BWI76_RS03135 0.75 -2.8 646,881 - BWI76_RS03135 0.88 -0.3 646,887 + BWI76_RS03135 0.89 -0.4 647,206 + +0.3 647,260 - -2.8 647,274 + +1.5 647,378 - BWI76_RS03140 0.20 -2.7 647,610 + BWI76_RS03140 0.43 +0.2 647,632 - BWI76_RS03140 0.45 +1.1 647,891 + BWI76_RS03140 0.70 +0.5 647,899 - BWI76_RS03140 0.71 +0.7 647,945 + BWI76_RS03140 0.76 -1.4 647,954 + BWI76_RS03140 0.77 +0.6 647,954 + BWI76_RS03140 0.77 -3.1 647,992 + BWI76_RS03140 0.80 +0.4 647,992 + BWI76_RS03140 0.80 -3.4 648,090 - BWI76_RS03140 0.90 +1.5 648,195 - -0.5 648,217 + +2.1 648,237 - -0.6 648,241 + +2.2 648,285 - -0.3 648,288 - +4.5 648,309 - +0.0 648,685 + +0.8 648,691 + +1.7 648,695 + -0.3 648,703 - -1.7 648,710 + -1.4 648,841 + BWI76_RS03150 0.13 -0.2 648,867 - BWI76_RS03150 0.15 -0.6 648,869 + BWI76_RS03150 0.15 -0.0 648,877 - BWI76_RS03150 0.16 -0.7 648,877 - BWI76_RS03150 0.16 -1.5 648,906 + BWI76_RS03150 0.18 +1.1 648,906 + BWI76_RS03150 0.18 +1.7 648,913 + BWI76_RS03150 0.19 -0.9 648,921 - BWI76_RS03150 0.19 +0.1 648,963 + BWI76_RS03150 0.23 -0.0 649,056 - BWI76_RS03150 0.30 -1.2 649,097 + BWI76_RS03150 0.33 -0.5 649,097 + BWI76_RS03150 0.33 +0.4 649,097 + BWI76_RS03150 0.33 -0.5 649,112 + BWI76_RS03150 0.35 -1.7 649,151 + BWI76_RS03150 0.38 +0.7 649,159 - BWI76_RS03150 0.38 -0.0
Or see this region's nucleotide sequence