Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01695 and BWI76_RS01700 are separated by 243 nucleotides BWI76_RS01700 and BWI76_RS01705 are separated by 236 nucleotides BWI76_RS01705 and BWI76_RS01710 overlap by 1 nucleotides BWI76_RS01710 and BWI76_RS01715 are separated by 31 nucleotides
BWI76_RS01695: BWI76_RS01695 - IS110 family transposase, at 348,913 to 349,893
_RS01695
BWI76_RS01700: BWI76_RS01700 - dipeptidase E, at 350,137 to 350,826
_RS01700
BWI76_RS01705: BWI76_RS01705 - CcdB antidote CcdA, at 351,063 to 351,356
_RS01705
BWI76_RS01710: BWI76_RS01710 - DNA gyrase inhibitor, at 351,356 to 351,673
_RS01710
BWI76_RS01715: BWI76_RS01715 - shikimate 5-dehydrogenase, at 351,705 to 352,514
_RS01715
Position (kb) 350
351 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 349.492 kb on - strand, within BWI76_RS01695 at 349.569 kb on + strand, within BWI76_RS01695 at 349.607 kb on + strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695 at 349.633 kb on - strand, within BWI76_RS01695 at 349.680 kb on - strand, within BWI76_RS01695 at 349.680 kb on - strand, within BWI76_RS01695 at 349.708 kb on + strand, within BWI76_RS01695 at 349.733 kb on - strand, within BWI76_RS01695 at 349.743 kb on - strand, within BWI76_RS01695 at 349.845 kb on - strand at 349.860 kb on + strand at 350.096 kb on - strand at 350.153 kb on + strand at 350.188 kb on - strand at 350.202 kb on - strand at 350.219 kb on + strand, within BWI76_RS01700 at 350.219 kb on + strand, within BWI76_RS01700 at 350.282 kb on - strand, within BWI76_RS01700 at 350.293 kb on - strand, within BWI76_RS01700 at 350.293 kb on - strand, within BWI76_RS01700 at 350.457 kb on - strand, within BWI76_RS01700 at 350.457 kb on - strand, within BWI76_RS01700 at 350.722 kb on - strand, within BWI76_RS01700 at 350.780 kb on - strand at 350.908 kb on - strand at 350.909 kb on + strand at 350.922 kb on - strand at 350.922 kb on - strand at 350.963 kb on - strand at 351.034 kb on - strand at 351.258 kb on + strand, within BWI76_RS01705 at 351.310 kb on + strand, within BWI76_RS01705 at 351.443 kb on + strand, within BWI76_RS01710 at 351.458 kb on - strand, within BWI76_RS01710 at 351.622 kb on + strand, within BWI76_RS01710 at 351.622 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.635 kb on - strand, within BWI76_RS01710 at 351.682 kb on + strand at 351.682 kb on + strand at 351.690 kb on - strand at 351.722 kb on - strand at 351.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 349,492 - BWI76_RS01695 0.59 -0.7 349,569 + BWI76_RS01695 0.67 -1.0 349,607 + BWI76_RS01695 0.71 -0.1 349,633 - BWI76_RS01695 0.73 +1.0 349,633 - BWI76_RS01695 0.73 +0.2 349,680 - BWI76_RS01695 0.78 -1.1 349,680 - BWI76_RS01695 0.78 +1.3 349,708 + BWI76_RS01695 0.81 -0.1 349,733 - BWI76_RS01695 0.84 -2.5 349,743 - BWI76_RS01695 0.85 -1.3 349,845 - -1.7 349,860 + +0.0 350,096 - +1.1 350,153 + -1.9 350,188 - -0.5 350,202 - +0.5 350,219 + BWI76_RS01700 0.12 +0.1 350,219 + BWI76_RS01700 0.12 -0.7 350,282 - BWI76_RS01700 0.21 +0.7 350,293 - BWI76_RS01700 0.23 +4.2 350,293 - BWI76_RS01700 0.23 +0.4 350,457 - BWI76_RS01700 0.46 +0.4 350,457 - BWI76_RS01700 0.46 -0.1 350,722 - BWI76_RS01700 0.85 -0.1 350,780 - -0.6 350,908 - +0.5 350,909 + -0.5 350,922 - -2.0 350,922 - +0.7 350,963 - +1.6 351,034 - -1.2 351,258 + BWI76_RS01705 0.66 -1.6 351,310 + BWI76_RS01705 0.84 +1.8 351,443 + BWI76_RS01710 0.27 +0.7 351,458 - BWI76_RS01710 0.32 -0.3 351,622 + BWI76_RS01710 0.84 +0.3 351,622 + BWI76_RS01710 0.84 +4.8 351,627 + BWI76_RS01710 0.85 -0.4 351,627 + BWI76_RS01710 0.85 -0.7 351,627 + BWI76_RS01710 0.85 -0.2 351,630 - BWI76_RS01710 0.86 -0.1 351,630 - BWI76_RS01710 0.86 -0.4 351,630 - BWI76_RS01710 0.86 -0.5 351,635 - BWI76_RS01710 0.88 -0.3 351,682 + -0.8 351,682 + -1.5 351,690 - +0.1 351,722 - +0.6 351,777 + +0.3
Or see this region's nucleotide sequence