Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01530 and BWI76_RS01535 overlap by 4 nucleotides BWI76_RS01535 and BWI76_RS01540 are separated by 1 nucleotides BWI76_RS01540 and BWI76_RS01545 overlap by 17 nucleotides BWI76_RS01545 and BWI76_RS01550 overlap by 8 nucleotides BWI76_RS01550 and BWI76_RS01555 overlap by 1 nucleotides
BWI76_RS01530: BWI76_RS01530 - 2-iminoacetate synthase ThiH, at 312,554 to 313,687
_RS01530
BWI76_RS01535: BWI76_RS01535 - thiazole synthase, at 313,684 to 314,454
_RS01535
BWI76_RS01540: BWI76_RS01540 - thiamine biosynthesis protein ThiS, at 314,456 to 314,656
_RS01540
BWI76_RS01545: BWI76_RS01545 - thiamine biosynthesis protein ThiS, at 314,640 to 315,395
_RS01545
BWI76_RS01550: BWI76_RS01550 - thiamine phosphate synthase, at 315,388 to 316,023
_RS01550
BWI76_RS01555: BWI76_RS01555 - phosphomethylpyrimidine synthase ThiC, at 316,023 to 317,918
_RS01555
Position (kb) 314
315
316 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 313.716 kb on + strand at 313.846 kb on + strand at 313.849 kb on - strand, within BWI76_RS01535 at 313.908 kb on - strand, within BWI76_RS01535 at 314.057 kb on - strand, within BWI76_RS01535 at 314.068 kb on - strand, within BWI76_RS01535 at 314.080 kb on + strand, within BWI76_RS01535 at 314.178 kb on + strand, within BWI76_RS01535 at 314.257 kb on - strand, within BWI76_RS01535 at 314.337 kb on + strand, within BWI76_RS01535 at 314.624 kb on + strand, within BWI76_RS01540 at 314.624 kb on + strand, within BWI76_RS01540 at 314.743 kb on + strand, within BWI76_RS01545 at 315.138 kb on - strand, within BWI76_RS01545 at 315.202 kb on - strand, within BWI76_RS01545 at 315.435 kb on + strand at 315.461 kb on + strand, within BWI76_RS01550 at 315.551 kb on - strand, within BWI76_RS01550 at 315.725 kb on - strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.779 kb on + strand, within BWI76_RS01550 at 315.912 kb on + strand, within BWI76_RS01550 at 316.029 kb on - strand at 316.138 kb on - strand at 316.326 kb on + strand, within BWI76_RS01555
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 313,716 + -0.2 313,846 + +0.6 313,849 - BWI76_RS01535 0.21 +0.5 313,908 - BWI76_RS01535 0.29 -1.6 314,057 - BWI76_RS01535 0.48 -0.2 314,068 - BWI76_RS01535 0.50 +1.0 314,080 + BWI76_RS01535 0.51 -0.5 314,178 + BWI76_RS01535 0.64 -1.2 314,257 - BWI76_RS01535 0.74 -1.1 314,337 + BWI76_RS01535 0.85 +1.1 314,624 + BWI76_RS01540 0.84 -0.9 314,624 + BWI76_RS01540 0.84 +5.2 314,743 + BWI76_RS01545 0.14 -2.0 315,138 - BWI76_RS01545 0.66 -0.3 315,202 - BWI76_RS01545 0.74 +3.1 315,435 + -1.8 315,461 + BWI76_RS01550 0.11 +0.6 315,551 - BWI76_RS01550 0.26 -0.1 315,725 - BWI76_RS01550 0.53 -2.3 315,779 + BWI76_RS01550 0.61 -0.7 315,779 + BWI76_RS01550 0.61 +0.3 315,779 + BWI76_RS01550 0.61 -0.9 315,912 + BWI76_RS01550 0.82 +2.2 316,029 - -2.1 316,138 - -0.3 316,326 + BWI76_RS01555 0.16 -0.8
Or see this region's nucleotide sequence