Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24825 and BWI76_RS24830 are separated by 28 nucleotides BWI76_RS24830 and BWI76_RS24835 are separated by 28 nucleotides BWI76_RS24835 and BWI76_RS24840 are separated by 392 nucleotides
BWI76_RS24825: BWI76_RS24825 - 2-hydroxy-3-oxopropionate reductase, at 5,172,634 to 5,173,524
_RS24825
BWI76_RS24830: BWI76_RS24830 - 5-keto-4-deoxy-D-glucarate aldolase, at 5,173,553 to 5,174,323
_RS24830
BWI76_RS24835: BWI76_RS24835 - MFS transporter, at 5,174,352 to 5,175,686
_RS24835
BWI76_RS24840: BWI76_RS24840 - galactarate dehydratase, at 5,176,079 to 5,177,650
_RS24840
Position (kb) 5174
5175
5176 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5173.533 kb on - strand at 5173.636 kb on - strand, within BWI76_RS24830 at 5173.639 kb on + strand, within BWI76_RS24830 at 5173.741 kb on + strand, within BWI76_RS24830 at 5173.777 kb on - strand, within BWI76_RS24830 at 5173.864 kb on - strand, within BWI76_RS24830 at 5173.923 kb on + strand, within BWI76_RS24830 at 5173.923 kb on + strand, within BWI76_RS24830 at 5174.191 kb on - strand, within BWI76_RS24830 at 5174.326 kb on + strand at 5174.334 kb on - strand at 5174.412 kb on + strand at 5174.446 kb on + strand at 5174.487 kb on - strand, within BWI76_RS24835 at 5174.586 kb on + strand, within BWI76_RS24835 at 5174.796 kb on - strand, within BWI76_RS24835 at 5174.814 kb on + strand, within BWI76_RS24835 at 5174.952 kb on - strand, within BWI76_RS24835 at 5175.082 kb on - strand, within BWI76_RS24835 at 5175.103 kb on - strand, within BWI76_RS24835 at 5175.116 kb on + strand, within BWI76_RS24835 at 5175.116 kb on + strand, within BWI76_RS24835 at 5175.208 kb on + strand at 5175.223 kb on + strand, within BWI76_RS24835 at 5175.234 kb on + strand, within BWI76_RS24835 at 5175.330 kb on - strand, within BWI76_RS24835 at 5175.353 kb on + strand, within BWI76_RS24835 at 5175.469 kb on + strand, within BWI76_RS24835 at 5175.506 kb on - strand, within BWI76_RS24835 at 5175.506 kb on - strand, within BWI76_RS24835 at 5175.522 kb on + strand, within BWI76_RS24835 at 5175.530 kb on - strand, within BWI76_RS24835 at 5175.530 kb on - strand, within BWI76_RS24835 at 5175.537 kb on + strand, within BWI76_RS24835 at 5175.540 kb on - strand, within BWI76_RS24835 at 5175.540 kb on - strand, within BWI76_RS24835 at 5175.540 kb on - strand, within BWI76_RS24835 at 5175.540 kb on - strand, within BWI76_RS24835 at 5175.547 kb on + strand, within BWI76_RS24835 at 5175.552 kb on + strand, within BWI76_RS24835 at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.557 kb on + strand at 5175.560 kb on + strand at 5175.560 kb on + strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.565 kb on - strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on + strand at 5175.568 kb on - strand at 5175.568 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.576 kb on - strand at 5175.578 kb on + strand at 5175.578 kb on + strand at 5175.586 kb on - strand at 5175.586 kb on - strand at 5175.587 kb on + strand at 5175.587 kb on + strand at 5175.595 kb on - strand at 5175.595 kb on - strand at 5175.652 kb on + strand at 5175.665 kb on + strand at 5175.704 kb on + strand at 5175.781 kb on + strand at 5175.797 kb on + strand at 5175.814 kb on - strand at 5175.816 kb on + strand at 5175.816 kb on + strand at 5175.824 kb on + strand at 5175.824 kb on - strand at 5175.824 kb on - strand at 5175.824 kb on - strand at 5175.824 kb on - strand at 5175.824 kb on - strand at 5175.826 kb on + strand at 5175.826 kb on + strand at 5175.826 kb on + strand at 5175.826 kb on + strand at 5175.827 kb on + strand at 5175.832 kb on - strand at 5175.832 kb on - strand at 5175.834 kb on - strand at 5175.834 kb on - strand at 5175.835 kb on - strand at 5175.835 kb on - strand at 5175.836 kb on + strand at 5175.886 kb on + strand at 5175.900 kb on + strand at 5175.938 kb on + strand at 5175.953 kb on - strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.957 kb on + strand at 5175.958 kb on + strand at 5175.959 kb on + strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.965 kb on - strand at 5175.982 kb on + strand at 5176.069 kb on + strand at 5176.077 kb on - strand at 5176.077 kb on - strand at 5176.127 kb on + strand at 5176.149 kb on - strand at 5176.160 kb on + strand at 5176.179 kb on - strand at 5176.179 kb on - strand at 5176.180 kb on - strand at 5176.194 kb on + strand at 5176.194 kb on + strand at 5176.194 kb on + strand at 5176.202 kb on - strand at 5176.203 kb on + strand at 5176.203 kb on + strand at 5176.203 kb on + strand at 5176.203 kb on + strand at 5176.203 kb on + strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.211 kb on - strand at 5176.213 kb on + strand at 5176.213 kb on + strand at 5176.213 kb on + strand at 5176.213 kb on + strand at 5176.215 kb on - strand at 5176.221 kb on - strand at 5176.231 kb on + strand at 5176.292 kb on + strand, within BWI76_RS24840 at 5176.292 kb on + strand, within BWI76_RS24840 at 5176.292 kb on + strand, within BWI76_RS24840 at 5176.300 kb on - strand, within BWI76_RS24840 at 5176.300 kb on - strand, within BWI76_RS24840 at 5176.315 kb on - strand, within BWI76_RS24840 at 5176.316 kb on + strand, within BWI76_RS24840 at 5176.324 kb on - strand, within BWI76_RS24840 at 5176.327 kb on - strand, within BWI76_RS24840 at 5176.356 kb on - strand, within BWI76_RS24840 at 5176.427 kb on + strand, within BWI76_RS24840 at 5176.507 kb on - strand, within BWI76_RS24840 at 5176.669 kb on - strand, within BWI76_RS24840
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,173,533 - +0.9 5,173,636 - BWI76_RS24830 0.11 -1.3 5,173,639 + BWI76_RS24830 0.11 -0.0 5,173,741 + BWI76_RS24830 0.24 +0.5 5,173,777 - BWI76_RS24830 0.29 +2.6 5,173,864 - BWI76_RS24830 0.40 -0.5 5,173,923 + BWI76_RS24830 0.48 +0.2 5,173,923 + BWI76_RS24830 0.48 -0.1 5,174,191 - BWI76_RS24830 0.83 +0.0 5,174,326 + -0.4 5,174,334 - +0.1 5,174,412 + -0.5 5,174,446 + +1.0 5,174,487 - BWI76_RS24835 0.10 -0.1 5,174,586 + BWI76_RS24835 0.18 -0.3 5,174,796 - BWI76_RS24835 0.33 +1.7 5,174,814 + BWI76_RS24835 0.35 -0.4 5,174,952 - BWI76_RS24835 0.45 -1.3 5,175,082 - BWI76_RS24835 0.55 -1.0 5,175,103 - BWI76_RS24835 0.56 +0.0 5,175,116 + BWI76_RS24835 0.57 +1.1 5,175,116 + BWI76_RS24835 0.57 +0.7 5,175,208 + +0.5 5,175,223 + BWI76_RS24835 0.65 -0.2 5,175,234 + BWI76_RS24835 0.66 +0.6 5,175,330 - BWI76_RS24835 0.73 -0.8 5,175,353 + BWI76_RS24835 0.75 +1.6 5,175,469 + BWI76_RS24835 0.84 +0.6 5,175,506 - BWI76_RS24835 0.86 -0.0 5,175,506 - BWI76_RS24835 0.86 -0.3 5,175,522 + BWI76_RS24835 0.88 +1.8 5,175,530 - BWI76_RS24835 0.88 -0.1 5,175,530 - BWI76_RS24835 0.88 +1.0 5,175,537 + BWI76_RS24835 0.89 -1.4 5,175,540 - BWI76_RS24835 0.89 +0.2 5,175,540 - BWI76_RS24835 0.89 -0.8 5,175,540 - BWI76_RS24835 0.89 -1.8 5,175,540 - BWI76_RS24835 0.89 -0.0 5,175,547 + BWI76_RS24835 0.90 -0.1 5,175,552 + BWI76_RS24835 0.90 -0.5 5,175,557 + -0.2 5,175,557 + +0.4 5,175,557 + +0.1 5,175,557 + +0.0 5,175,557 + +0.6 5,175,557 + -0.1 5,175,557 + -0.5 5,175,557 + +0.2 5,175,557 + -0.9 5,175,557 + -1.4 5,175,557 + -1.0 5,175,557 + -0.2 5,175,557 + +2.9 5,175,557 + +0.9 5,175,557 + -0.0 5,175,557 + +2.5 5,175,557 + +0.3 5,175,560 + +0.2 5,175,560 + +0.4 5,175,565 - +0.0 5,175,565 - +0.5 5,175,565 - +0.3 5,175,565 - -0.3 5,175,565 - -0.4 5,175,565 - -0.0 5,175,565 - -0.6 5,175,565 - +1.0 5,175,565 - -0.3 5,175,565 - -0.0 5,175,565 - +0.2 5,175,565 - +0.5 5,175,565 - -0.6 5,175,565 - -0.3 5,175,565 - -0.3 5,175,565 - -1.0 5,175,568 + +0.2 5,175,568 + +1.2 5,175,568 + +0.6 5,175,568 + -0.4 5,175,568 + -0.9 5,175,568 + -0.3 5,175,568 + -0.7 5,175,568 + +0.3 5,175,568 + -0.1 5,175,568 + -0.4 5,175,568 - -0.5 5,175,568 - +0.8 5,175,576 - -0.8 5,175,576 - -0.4 5,175,576 - -0.7 5,175,576 - -0.2 5,175,576 - -0.2 5,175,576 - +1.1 5,175,578 + +0.6 5,175,578 + +0.1 5,175,586 - +0.2 5,175,586 - +0.2 5,175,587 + -1.4 5,175,587 + -0.6 5,175,595 - -0.2 5,175,595 - +0.4 5,175,652 + -0.7 5,175,665 + -0.3 5,175,704 + +1.9 5,175,781 + -0.4 5,175,797 + -1.5 5,175,814 - -1.5 5,175,816 + -0.2 5,175,816 + +0.6 5,175,824 + +1.0 5,175,824 - +0.6 5,175,824 - +1.7 5,175,824 - -0.4 5,175,824 - -0.1 5,175,824 - -0.5 5,175,826 + +1.2 5,175,826 + -0.6 5,175,826 + +0.7 5,175,826 + -0.0 5,175,827 + +3.1 5,175,832 - -0.0 5,175,832 - -0.9 5,175,834 - +0.5 5,175,834 - +0.3 5,175,835 - -1.0 5,175,835 - -0.1 5,175,836 + +0.3 5,175,886 + -1.2 5,175,900 + -0.3 5,175,938 + -0.5 5,175,953 - -0.7 5,175,957 + -0.3 5,175,957 + +0.7 5,175,957 + +1.8 5,175,957 + +1.7 5,175,957 + -2.6 5,175,957 + -0.3 5,175,957 + -0.2 5,175,958 + -0.1 5,175,959 + -0.5 5,175,965 - -0.7 5,175,965 - +0.1 5,175,965 - +2.8 5,175,965 - +0.7 5,175,965 - -2.5 5,175,965 - -0.6 5,175,965 - -0.5 5,175,965 - -0.4 5,175,982 + -0.2 5,176,069 + +0.3 5,176,077 - -0.2 5,176,077 - -0.3 5,176,127 + +0.4 5,176,149 - -0.8 5,176,160 + +0.3 5,176,179 - -0.1 5,176,179 - +1.2 5,176,180 - -1.0 5,176,194 + +1.7 5,176,194 + +0.3 5,176,194 + +0.4 5,176,202 - -0.4 5,176,203 + +0.9 5,176,203 + +0.3 5,176,203 + +0.0 5,176,203 + +0.4 5,176,203 + -0.1 5,176,211 - -0.8 5,176,211 - +0.6 5,176,211 - -0.3 5,176,211 - +0.3 5,176,211 - +0.6 5,176,211 - -1.2 5,176,211 - -0.5 5,176,211 - -0.7 5,176,213 + -0.1 5,176,213 + -1.0 5,176,213 + -0.3 5,176,213 + +0.1 5,176,215 - +0.3 5,176,221 - -0.7 5,176,231 + +0.7 5,176,292 + BWI76_RS24840 0.14 -2.4 5,176,292 + BWI76_RS24840 0.14 -0.5 5,176,292 + BWI76_RS24840 0.14 +0.4 5,176,300 - BWI76_RS24840 0.14 -0.3 5,176,300 - BWI76_RS24840 0.14 +2.0 5,176,315 - BWI76_RS24840 0.15 -0.3 5,176,316 + BWI76_RS24840 0.15 -0.8 5,176,324 - BWI76_RS24840 0.16 -1.3 5,176,327 - BWI76_RS24840 0.16 -0.7 5,176,356 - BWI76_RS24840 0.18 -0.4 5,176,427 + BWI76_RS24840 0.22 +2.7 5,176,507 - BWI76_RS24840 0.27 +0.2 5,176,669 - BWI76_RS24840 0.38 -0.1
Or see this region's nucleotide sequence