Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23855 and BWI76_RS23860 are separated by 190 nucleotides BWI76_RS23860 and BWI76_RS23865 are separated by 186 nucleotides BWI76_RS23865 and BWI76_RS23870 are separated by 60 nucleotides
BWI76_RS23855: BWI76_RS23855 - 6-phospho-beta-glucosidase, at 4,977,365 to 4,978,798
_RS23855
BWI76_RS23860: BWI76_RS23860 - protein disulfide oxidoreductase, at 4,978,989 to 4,979,480
_RS23860
BWI76_RS23865: BWI76_RS23865 - NAD(P)-dependent oxidoreductase, at 4,979,667 to 4,980,410
_RS23865
BWI76_RS23870: BWI76_RS23870 - Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data) , at 4,980,471 to 4,983,344
_RS23870
Position (kb)
4979
4980
4981 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 4978.672 kb on - strand at 4978.686 kb on + strand at 4978.834 kb on - strand at 4979.169 kb on + strand, within BWI76_RS23860 at 4979.524 kb on + strand at 4979.556 kb on - strand at 4979.580 kb on + strand at 4979.590 kb on + strand at 4979.598 kb on - strand at 4979.606 kb on - strand at 4979.844 kb on + strand, within BWI76_RS23865 at 4980.004 kb on + strand, within BWI76_RS23865 at 4980.012 kb on - strand, within BWI76_RS23865 at 4980.038 kb on + strand, within BWI76_RS23865 at 4980.154 kb on + strand, within BWI76_RS23865 at 4980.162 kb on - strand, within BWI76_RS23865 at 4980.213 kb on + strand, within BWI76_RS23865 at 4980.309 kb on - strand, within BWI76_RS23865 at 4980.309 kb on - strand, within BWI76_RS23865 at 4980.339 kb on - strand at 4980.564 kb on + strand at 4980.778 kb on - strand, within BWI76_RS23870 at 4981.058 kb on + strand, within BWI76_RS23870 at 4981.219 kb on + strand, within BWI76_RS23870 at 4981.236 kb on - strand, within BWI76_RS23870 at 4981.273 kb on + strand, within BWI76_RS23870
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 4,978,672 - -0.4 4,978,686 + -0.1 4,978,834 - -0.3 4,979,169 + BWI76_RS23860 0.37 -0.4 4,979,524 + +0.2 4,979,556 - +0.4 4,979,580 + -1.4 4,979,590 + +0.2 4,979,598 - +1.1 4,979,606 - -0.4 4,979,844 + BWI76_RS23865 0.24 -0.5 4,980,004 + BWI76_RS23865 0.45 -0.3 4,980,012 - BWI76_RS23865 0.46 -0.2 4,980,038 + BWI76_RS23865 0.50 -0.8 4,980,154 + BWI76_RS23865 0.65 +6.0 4,980,162 - BWI76_RS23865 0.67 +1.5 4,980,213 + BWI76_RS23865 0.73 +0.1 4,980,309 - BWI76_RS23865 0.86 -0.6 4,980,309 - BWI76_RS23865 0.86 -0.4 4,980,339 - -1.3 4,980,564 + -1.8 4,980,778 - BWI76_RS23870 0.11 -0.5 4,981,058 + BWI76_RS23870 0.20 -0.5 4,981,219 + BWI76_RS23870 0.26 -1.7 4,981,236 - BWI76_RS23870 0.27 -2.0 4,981,273 + BWI76_RS23870 0.28 -1.3
Or see this region's nucleotide sequence