Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13405 and BWI76_RS13410 are separated by 19 nucleotides BWI76_RS13410 and BWI76_RS13415 are separated by 1 nucleotides BWI76_RS13415 and BWI76_RS13420 overlap by 11 nucleotides
BWI76_RS13405: BWI76_RS13405 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,796,103 to 2,797,248
_RS13405
BWI76_RS13410: BWI76_RS13410 - polyamine ABC transporter ATP-binding protein, at 2,797,268 to 2,798,278
_RS13410
BWI76_RS13415: BWI76_RS13415 - spermidine/putrescine ABC transporter permease, at 2,798,280 to 2,799,209
_RS13415
BWI76_RS13420: BWI76_RS13420 - ABC transporter permease, at 2,799,199 to 2,800,005
_RS13420
Position (kb)
2797
2798
2799 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2796.362 kb on + strand, within BWI76_RS13405 at 2796.370 kb on - strand, within BWI76_RS13405 at 2796.510 kb on + strand, within BWI76_RS13405 at 2796.946 kb on + strand, within BWI76_RS13405 at 2796.985 kb on + strand, within BWI76_RS13405 at 2797.092 kb on + strand, within BWI76_RS13405 at 2797.769 kb on - strand, within BWI76_RS13410 at 2797.780 kb on + strand, within BWI76_RS13410 at 2797.839 kb on + strand, within BWI76_RS13410 at 2798.327 kb on + strand at 2798.475 kb on - strand, within BWI76_RS13415 at 2798.596 kb on + strand, within BWI76_RS13415 at 2798.604 kb on - strand, within BWI76_RS13415 at 2798.693 kb on - strand, within BWI76_RS13415 at 2799.088 kb on + strand, within BWI76_RS13415
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 2,796,362 + BWI76_RS13405 0.23 +0.3 2,796,370 - BWI76_RS13405 0.23 +1.2 2,796,510 + BWI76_RS13405 0.36 -0.4 2,796,946 + BWI76_RS13405 0.74 +0.1 2,796,985 + BWI76_RS13405 0.77 -1.1 2,797,092 + BWI76_RS13405 0.86 -0.5 2,797,769 - BWI76_RS13410 0.50 +0.0 2,797,780 + BWI76_RS13410 0.51 +0.3 2,797,839 + BWI76_RS13410 0.56 +5.5 2,798,327 + -0.1 2,798,475 - BWI76_RS13415 0.21 +0.1 2,798,596 + BWI76_RS13415 0.34 +0.9 2,798,604 - BWI76_RS13415 0.35 -0.3 2,798,693 - BWI76_RS13415 0.44 -1.1 2,799,088 + BWI76_RS13415 0.87 +1.6
Or see this region's nucleotide sequence