Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05470 and BWI76_RS05475 are separated by 238 nucleotides BWI76_RS05475 and BWI76_RS05480 are separated by 43 nucleotides BWI76_RS05480 and BWI76_RS05485 overlap by 30 nucleotides
BWI76_RS05470: BWI76_RS05470 - acyl-CoA dehydrogenase, at 1,146,634 to 1,149,078
_RS05470
BWI76_RS05475: BWI76_RS05475 - phosphoheptose isomerase, at 1,149,317 to 1,149,898
_RS05475
BWI76_RS05480: BWI76_RS05480 - class II glutamine amidotransferase, at 1,149,942 to 1,150,709
_RS05480
BWI76_RS05485: BWI76_RS05485 - transpeptidase, at 1,150,680 to 1,151,420
_RS05485
Position (kb)
1149
1150
1151 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1149.270 kb on + strand at 1150.067 kb on + strand, within BWI76_RS05480 at 1150.068 kb on + strand, within BWI76_RS05480 at 1150.076 kb on - strand, within BWI76_RS05480 at 1150.252 kb on - strand, within BWI76_RS05480 at 1150.474 kb on - strand, within BWI76_RS05480 at 1151.107 kb on + strand, within BWI76_RS05485 at 1151.179 kb on - strand, within BWI76_RS05485 at 1151.179 kb on - strand, within BWI76_RS05485 at 1151.186 kb on + strand, within BWI76_RS05485 at 1151.214 kb on + strand, within BWI76_RS05485 at 1151.284 kb on + strand, within BWI76_RS05485 at 1151.299 kb on + strand, within BWI76_RS05485 at 1151.341 kb on - strand, within BWI76_RS05485 at 1151.518 kb on - strand at 1151.699 kb on + strand at 1151.707 kb on - strand at 1151.707 kb on - strand at 1151.707 kb on - strand at 1151.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 1,149,270 + +0.1 1,150,067 + BWI76_RS05480 0.16 -0.2 1,150,068 + BWI76_RS05480 0.16 -0.9 1,150,076 - BWI76_RS05480 0.17 +4.5 1,150,252 - BWI76_RS05480 0.40 +0.3 1,150,474 - BWI76_RS05480 0.69 +0.0 1,151,107 + BWI76_RS05485 0.58 -0.6 1,151,179 - BWI76_RS05485 0.67 -0.4 1,151,179 - BWI76_RS05485 0.67 +0.8 1,151,186 + BWI76_RS05485 0.68 -1.6 1,151,214 + BWI76_RS05485 0.72 -0.1 1,151,284 + BWI76_RS05485 0.82 -0.3 1,151,299 + BWI76_RS05485 0.84 +0.1 1,151,341 - BWI76_RS05485 0.89 -0.6 1,151,518 - +0.3 1,151,699 + -0.3 1,151,707 - -0.0 1,151,707 - -0.4 1,151,707 - -2.0 1,151,707 - -1.6
Or see this region's nucleotide sequence