Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04355

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04345 and BWI76_RS04350 are separated by 54 nucleotidesBWI76_RS04350 and BWI76_RS04355 are separated by 1 nucleotidesBWI76_RS04355 and BWI76_RS04360 are separated by 65 nucleotides BWI76_RS04345: BWI76_RS04345 - signal peptidase II, at 895,864 to 896,364 _RS04345 BWI76_RS04350: BWI76_RS04350 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 896,419 to 896,868 _RS04350 BWI76_RS04355: BWI76_RS04355 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 896,870 to 897,820 _RS04355 BWI76_RS04360: BWI76_RS04360 - ribonucleoside hydrolase RihC, at 897,886 to 898,800 _RS04360 Position (kb) 896 897 898Strain fitness (log2 ratio) -1 0 1 2 3 4at 896.421 kb on + strandat 896.558 kb on + strand, within BWI76_RS04350at 897.853 kb on + strandat 897.853 kb on + strandat 898.063 kb on - strand, within BWI76_RS04360at 898.115 kb on - strand, within BWI76_RS04360at 898.350 kb on + strand, within BWI76_RS04360at 898.453 kb on - strandat 898.558 kb on + strand, within BWI76_RS04360at 898.628 kb on + strand, within BWI76_RS04360

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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896,421 + -0.1
896,558 + BWI76_RS04350 0.31 +2.3
897,853 + +0.7
897,853 + -0.3
898,063 - BWI76_RS04360 0.19 -0.0
898,115 - BWI76_RS04360 0.25 +4.7
898,350 + BWI76_RS04360 0.51 +0.5
898,453 - +1.1
898,558 + BWI76_RS04360 0.73 -0.4
898,628 + BWI76_RS04360 0.81 +0.8

Or see this region's nucleotide sequence