Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00070 and BWI76_RS00075 are separated by 50 nucleotides BWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotides BWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotides BWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotides
BWI76_RS00070: BWI76_RS00070 - MFS transporter, at 13,479 to 14,969
_RS00070
BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709
_RS00075
BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581
_RS00080
BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042
_RS00085
BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393
_RS00090
Position (kb)
15
16
17 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 14.759 kb on + strand, within BWI76_RS00070 at 14.884 kb on + strand at 14.922 kb on - strand at 14.925 kb on - strand at 14.925 kb on - strand at 15.018 kb on - strand at 15.089 kb on + strand, within BWI76_RS00075 at 15.320 kb on - strand, within BWI76_RS00075 at 15.361 kb on + strand, within BWI76_RS00075 at 16.009 kb on + strand, within BWI76_RS00080 at 16.225 kb on + strand, within BWI76_RS00080 at 16.451 kb on + strand, within BWI76_RS00080 at 16.461 kb on + strand, within BWI76_RS00080 at 16.469 kb on - strand, within BWI76_RS00080 at 16.514 kb on - strand at 16.529 kb on - strand at 16.601 kb on - strand at 16.609 kb on + strand at 16.616 kb on + strand at 16.631 kb on + strand, within BWI76_RS00085 at 16.631 kb on + strand, within BWI76_RS00085 at 16.932 kb on - strand, within BWI76_RS00085 at 17.020 kb on - strand at 17.088 kb on - strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.184 kb on - strand at 17.184 kb on - strand at 17.214 kb on - strand at 17.244 kb on + strand at 17.252 kb on - strand at 17.252 kb on - strand at 17.498 kb on - strand, within BWI76_RS00090 at 17.539 kb on + strand, within BWI76_RS00090 at 17.546 kb on + strand, within BWI76_RS00090
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 14,759 + BWI76_RS00070 0.86 +2.6 14,884 + -1.0 14,922 - -1.2 14,925 - +0.0 14,925 - -0.8 15,018 - +1.9 15,089 + BWI76_RS00075 0.10 -0.8 15,320 - BWI76_RS00075 0.43 -0.4 15,361 + BWI76_RS00075 0.49 -1.7 16,009 + BWI76_RS00080 0.35 +0.1 16,225 + BWI76_RS00080 0.59 -0.7 16,451 + BWI76_RS00080 0.85 -0.3 16,461 + BWI76_RS00080 0.86 +1.3 16,469 - BWI76_RS00080 0.87 -0.6 16,514 - -0.4 16,529 - -1.0 16,601 - -0.7 16,609 + +1.2 16,616 + +0.1 16,631 + BWI76_RS00085 0.11 -0.9 16,631 + BWI76_RS00085 0.11 -0.1 16,932 - BWI76_RS00085 0.76 +0.4 17,020 - -0.3 17,088 - +0.1 17,176 + +0.5 17,176 + -0.1 17,176 + +0.6 17,176 + +2.0 17,184 - +0.2 17,184 - -0.4 17,214 - -0.8 17,244 + -0.4 17,252 - -0.9 17,252 - +0.2 17,498 - BWI76_RS00090 0.24 -2.3 17,539 + BWI76_RS00090 0.28 -0.4 17,546 + BWI76_RS00090 0.28 +1.5
Or see this region's nucleotide sequence