Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00065 and BWI76_RS00070 are separated by 326 nucleotides BWI76_RS00070 and BWI76_RS00075 are separated by 50 nucleotides BWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotides
BWI76_RS00065: BWI76_RS00065 - MurR/RpiR family transcriptional regulator, at 12,271 to 13,152
_RS00065
BWI76_RS00070: BWI76_RS00070 - MFS transporter, at 13,479 to 14,969
_RS00070
BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709
_RS00075
BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581
_RS00080
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 12.527 kb on - strand, within BWI76_RS00065 at 12.584 kb on + strand, within BWI76_RS00065 at 12.584 kb on + strand, within BWI76_RS00065 at 12.695 kb on - strand, within BWI76_RS00065 at 12.749 kb on + strand, within BWI76_RS00065 at 12.749 kb on + strand, within BWI76_RS00065 at 12.822 kb on + strand, within BWI76_RS00065 at 13.124 kb on + strand at 13.253 kb on + strand at 13.471 kb on - strand at 13.471 kb on - strand at 13.575 kb on + strand at 13.624 kb on + strand at 13.648 kb on - strand, within BWI76_RS00070 at 13.693 kb on + strand, within BWI76_RS00070 at 13.794 kb on - strand, within BWI76_RS00070 at 13.842 kb on - strand, within BWI76_RS00070 at 13.858 kb on + strand, within BWI76_RS00070 at 13.858 kb on + strand, within BWI76_RS00070 at 13.980 kb on - strand, within BWI76_RS00070 at 13.993 kb on - strand, within BWI76_RS00070 at 14.009 kb on - strand, within BWI76_RS00070 at 14.184 kb on + strand, within BWI76_RS00070 at 14.246 kb on + strand, within BWI76_RS00070 at 14.277 kb on + strand, within BWI76_RS00070 at 14.277 kb on - strand, within BWI76_RS00070 at 14.285 kb on - strand, within BWI76_RS00070 at 14.285 kb on - strand, within BWI76_RS00070 at 14.285 kb on - strand, within BWI76_RS00070 at 14.389 kb on - strand, within BWI76_RS00070 at 14.548 kb on - strand, within BWI76_RS00070 at 14.607 kb on - strand, within BWI76_RS00070 at 14.673 kb on - strand, within BWI76_RS00070 at 14.759 kb on + strand, within BWI76_RS00070 at 14.884 kb on + strand at 14.922 kb on - strand at 14.925 kb on - strand at 14.925 kb on - strand at 15.018 kb on - strand at 15.089 kb on + strand, within BWI76_RS00075 at 15.320 kb on - strand, within BWI76_RS00075 at 15.361 kb on + strand, within BWI76_RS00075
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 12,527 - BWI76_RS00065 0.29 -1.9 12,584 + BWI76_RS00065 0.35 +0.7 12,584 + BWI76_RS00065 0.35 +0.0 12,695 - BWI76_RS00065 0.48 -0.8 12,749 + BWI76_RS00065 0.54 -2.3 12,749 + BWI76_RS00065 0.54 -1.5 12,822 + BWI76_RS00065 0.62 -1.4 13,124 + -1.8 13,253 + -0.3 13,471 - +0.3 13,471 - -0.5 13,575 + -0.6 13,624 + -1.0 13,648 - BWI76_RS00070 0.11 -0.3 13,693 + BWI76_RS00070 0.14 +0.8 13,794 - BWI76_RS00070 0.21 -0.2 13,842 - BWI76_RS00070 0.24 -1.4 13,858 + BWI76_RS00070 0.25 -2.7 13,858 + BWI76_RS00070 0.25 -0.9 13,980 - BWI76_RS00070 0.34 -1.0 13,993 - BWI76_RS00070 0.34 +1.1 14,009 - BWI76_RS00070 0.36 +2.0 14,184 + BWI76_RS00070 0.47 +1.6 14,246 + BWI76_RS00070 0.51 -0.4 14,277 + BWI76_RS00070 0.54 -0.3 14,277 - BWI76_RS00070 0.54 +0.3 14,285 - BWI76_RS00070 0.54 -0.4 14,285 - BWI76_RS00070 0.54 -0.4 14,285 - BWI76_RS00070 0.54 +0.5 14,389 - BWI76_RS00070 0.61 +2.0 14,548 - BWI76_RS00070 0.72 +0.1 14,607 - BWI76_RS00070 0.76 -0.8 14,673 - BWI76_RS00070 0.80 +0.7 14,759 + BWI76_RS00070 0.86 +2.6 14,884 + -1.0 14,922 - -1.2 14,925 - +0.0 14,925 - -0.8 15,018 - +1.9 15,089 + BWI76_RS00075 0.10 -0.8 15,320 - BWI76_RS00075 0.43 -0.4 15,361 + BWI76_RS00075 0.49 -1.7
Or see this region's nucleotide sequence