Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25645 and BWI76_RS25650 are separated by 3 nucleotides BWI76_RS25650 and BWI76_RS25655 are separated by 4 nucleotides BWI76_RS25655 and BWI76_RS25660 are separated by 27 nucleotides BWI76_RS25660 and BWI76_RS25665 overlap by 29 nucleotides
BWI76_RS25645: BWI76_RS25645 - shikimate dehydrogenase, at 5,326,644 to 5,327,462
_RS25645
BWI76_RS25650: BWI76_RS25650 - L-threonylcarbamoyladenylate synthase type 1 TsaC, at 5,327,466 to 5,328,038
_RS25650
BWI76_RS25655: BWI76_RS25655 - DNA topoisomerase type IA zn finger domain-containing protein, at 5,328,043 to 5,328,585
_RS25655
BWI76_RS25660: BWI76_RS25660 - hypothetical protein, at 5,328,613 to 5,329,086
_RS25660
BWI76_RS25665: BWI76_RS25665 - DNA-processing protein DprA, at 5,329,058 to 5,330,182
_RS25665
Position (kb) 5328
5329 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8 at 5327.065 kb on - strand, within BWI76_RS25645 at 5327.139 kb on + strand, within BWI76_RS25645 at 5327.147 kb on + strand, within BWI76_RS25645 at 5327.147 kb on + strand, within BWI76_RS25645 at 5327.147 kb on - strand, within BWI76_RS25645 at 5327.193 kb on - strand, within BWI76_RS25645 at 5327.217 kb on + strand, within BWI76_RS25645 at 5327.217 kb on + strand, within BWI76_RS25645 at 5327.225 kb on - strand, within BWI76_RS25645 at 5327.301 kb on - strand, within BWI76_RS25645 at 5327.362 kb on - strand, within BWI76_RS25645 at 5328.405 kb on - strand, within BWI76_RS25655 at 5328.414 kb on - strand, within BWI76_RS25655 at 5328.470 kb on + strand at 5328.492 kb on - strand, within BWI76_RS25655 at 5328.520 kb on - strand, within BWI76_RS25655 at 5328.763 kb on + strand, within BWI76_RS25660 at 5328.866 kb on + strand, within BWI76_RS25660 at 5328.951 kb on + strand, within BWI76_RS25660 at 5329.015 kb on + strand, within BWI76_RS25660 at 5329.023 kb on - strand, within BWI76_RS25660 at 5329.055 kb on + strand at 5329.133 kb on - strand at 5329.140 kb on + strand at 5329.153 kb on - strand at 5329.215 kb on + strand, within BWI76_RS25665 at 5329.218 kb on - strand, within BWI76_RS25665 at 5329.224 kb on - strand, within BWI76_RS25665 at 5329.345 kb on - strand, within BWI76_RS25665 at 5329.401 kb on - strand, within BWI76_RS25665
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,327,065 - BWI76_RS25645 0.51 -0.1 5,327,139 + BWI76_RS25645 0.60 +0.4 5,327,147 + BWI76_RS25645 0.61 -0.4 5,327,147 + BWI76_RS25645 0.61 +0.9 5,327,147 - BWI76_RS25645 0.61 -1.0 5,327,193 - BWI76_RS25645 0.67 +7.8 5,327,217 + BWI76_RS25645 0.70 -0.7 5,327,217 + BWI76_RS25645 0.70 +0.4 5,327,225 - BWI76_RS25645 0.71 -0.1 5,327,301 - BWI76_RS25645 0.80 -0.3 5,327,362 - BWI76_RS25645 0.88 -1.0 5,328,405 - BWI76_RS25655 0.67 -0.8 5,328,414 - BWI76_RS25655 0.68 +3.3 5,328,470 + +1.1 5,328,492 - BWI76_RS25655 0.83 +0.1 5,328,520 - BWI76_RS25655 0.88 +0.5 5,328,763 + BWI76_RS25660 0.32 -0.2 5,328,866 + BWI76_RS25660 0.53 -0.4 5,328,951 + BWI76_RS25660 0.71 +1.2 5,329,015 + BWI76_RS25660 0.85 -0.1 5,329,023 - BWI76_RS25660 0.86 -1.2 5,329,055 + +0.2 5,329,133 - +0.1 5,329,140 + +0.1 5,329,153 - +0.7 5,329,215 + BWI76_RS25665 0.14 +0.1 5,329,218 - BWI76_RS25665 0.14 +0.0 5,329,224 - BWI76_RS25665 0.15 +0.5 5,329,345 - BWI76_RS25665 0.26 -0.8 5,329,401 - BWI76_RS25665 0.30 -0.2
Or see this region's nucleotide sequence