Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22225 and BWI76_RS22230 are separated by 17 nucleotides BWI76_RS22230 and BWI76_RS22235 are separated by 133 nucleotides BWI76_RS22235 and BWI76_RS22240 are separated by 68 nucleotides BWI76_RS22240 and BWI76_RS22245 overlap by 8 nucleotides
BWI76_RS22225: BWI76_RS22225 - PTS sorbitol transporter subunit IIA, at 4,633,675 to 4,634,037
_RS22225
BWI76_RS22230: BWI76_RS22230 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) <small>(from data)</small>, at 4,634,055 to 4,634,834
_RS22230
BWI76_RS22235: BWI76_RS22235 - transcriptional regulator GutM, at 4,634,968 to 4,635,327
_RS22235
BWI76_RS22240: BWI76_RS22240 - transcriptional regulator, at 4,635,396 to 4,636,169
_RS22240
BWI76_RS22245: BWI76_RS22245 - arabinose 5-phosphate isomerase GutQ, at 4,636,162 to 4,637,127
_RS22245
Position (kb) 4634
4635
4636 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4633.977 kb on - strand, within BWI76_RS22225 at 4634.085 kb on + strand at 4634.184 kb on + strand, within BWI76_RS22230 at 4634.454 kb on - strand, within BWI76_RS22230 at 4634.508 kb on + strand, within BWI76_RS22230 at 4634.581 kb on + strand, within BWI76_RS22230 at 4634.664 kb on - strand, within BWI76_RS22230 at 4634.679 kb on - strand, within BWI76_RS22230 at 4635.182 kb on - strand, within BWI76_RS22235 at 4635.467 kb on - strand at 4635.495 kb on - strand, within BWI76_RS22240 at 4635.583 kb on - strand, within BWI76_RS22240 at 4635.705 kb on + strand, within BWI76_RS22240 at 4635.964 kb on - strand, within BWI76_RS22240 at 4636.176 kb on + strand at 4636.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 4,633,977 - BWI76_RS22225 0.83 -0.5 4,634,085 + +1.2 4,634,184 + BWI76_RS22230 0.17 +1.4 4,634,454 - BWI76_RS22230 0.51 +0.0 4,634,508 + BWI76_RS22230 0.58 +3.4 4,634,581 + BWI76_RS22230 0.67 +0.5 4,634,664 - BWI76_RS22230 0.78 -1.3 4,634,679 - BWI76_RS22230 0.80 +0.0 4,635,182 - BWI76_RS22235 0.59 +0.1 4,635,467 - -2.3 4,635,495 - BWI76_RS22240 0.13 -2.5 4,635,583 - BWI76_RS22240 0.24 -1.6 4,635,705 + BWI76_RS22240 0.40 -1.9 4,635,964 - BWI76_RS22240 0.73 -1.2 4,636,176 + -2.3 4,636,196 + -0.0
Or see this region's nucleotide sequence