Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26655 and BWI76_RS26660 are separated by 101 nucleotides BWI76_RS26660 and BWI76_RS26665 are separated by 237 nucleotides BWI76_RS26665 and BWI76_RS26670 overlap by 4 nucleotides
BWI76_RS26655: BWI76_RS26655 - transcription accessory protein, at 5,517,663 to 5,519,993
_RS26655
BWI76_RS26660: BWI76_RS26660 - transcription elongation factor GreB, at 5,520,095 to 5,520,568
_RS26660
BWI76_RS26665: BWI76_RS26665 - DNA-binding response regulator, at 5,520,806 to 5,521,525
_RS26665
BWI76_RS26670: BWI76_RS26670 - two-component sensor histidine kinase, at 5,521,522 to 5,522,883
_RS26670
Position (kb)
5520
5521
5522 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5519.819 kb on - strand at 5519.899 kb on - strand at 5519.904 kb on - strand at 5519.926 kb on + strand at 5519.926 kb on + strand at 5519.926 kb on + strand at 5519.934 kb on - strand at 5519.936 kb on + strand at 5519.936 kb on + strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.949 kb on - strand at 5519.954 kb on - strand at 5520.040 kb on + strand at 5520.177 kb on + strand, within BWI76_RS26660 at 5520.223 kb on - strand, within BWI76_RS26660 at 5520.366 kb on - strand, within BWI76_RS26660 at 5520.467 kb on + strand, within BWI76_RS26660 at 5520.467 kb on + strand, within BWI76_RS26660 at 5520.509 kb on - strand, within BWI76_RS26660 at 5520.541 kb on + strand at 5520.541 kb on + strand at 5520.549 kb on - strand at 5520.549 kb on - strand at 5520.581 kb on - strand at 5520.582 kb on - strand at 5520.595 kb on + strand at 5520.603 kb on - strand at 5520.663 kb on + strand at 5520.664 kb on + strand at 5520.719 kb on + strand at 5520.729 kb on - strand at 5520.788 kb on + strand at 5520.795 kb on - strand at 5520.795 kb on - strand at 5520.796 kb on + strand at 5520.797 kb on - strand at 5520.851 kb on - strand at 5520.862 kb on + strand at 5520.896 kb on - strand, within BWI76_RS26665 at 5520.917 kb on + strand, within BWI76_RS26665 at 5520.953 kb on + strand, within BWI76_RS26665 at 5521.121 kb on - strand, within BWI76_RS26665 at 5521.340 kb on + strand, within BWI76_RS26665 at 5521.512 kb on + strand at 5521.520 kb on - strand at 5521.573 kb on - strand at 5521.585 kb on - strand at 5521.595 kb on - strand at 5521.621 kb on - strand at 5521.646 kb on + strand at 5521.654 kb on - strand at 5521.666 kb on + strand, within BWI76_RS26670 at 5521.813 kb on + strand, within BWI76_RS26670 at 5521.821 kb on - strand, within BWI76_RS26670 at 5521.821 kb on - strand, within BWI76_RS26670 at 5521.958 kb on + strand, within BWI76_RS26670 at 5521.966 kb on - strand, within BWI76_RS26670 at 5521.966 kb on - strand, within BWI76_RS26670 at 5522.084 kb on + strand, within BWI76_RS26670 at 5522.150 kb on + strand, within BWI76_RS26670 at 5522.177 kb on + strand, within BWI76_RS26670 at 5522.219 kb on + strand, within BWI76_RS26670 at 5522.269 kb on - strand, within BWI76_RS26670 at 5522.349 kb on + strand, within BWI76_RS26670 at 5522.357 kb on - strand, within BWI76_RS26670 at 5522.400 kb on - strand, within BWI76_RS26670 at 5522.421 kb on + strand, within BWI76_RS26670 at 5522.422 kb on - strand, within BWI76_RS26670 at 5522.439 kb on - strand, within BWI76_RS26670
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,519,819 - -1.7 5,519,899 - -1.2 5,519,904 - +0.0 5,519,926 + -0.3 5,519,926 + -0.2 5,519,926 + +0.2 5,519,934 - +0.1 5,519,936 + +0.5 5,519,936 + -1.1 5,519,944 - -0.5 5,519,944 - -1.2 5,519,944 - -0.2 5,519,944 - -0.6 5,519,944 - -0.2 5,519,944 - -0.7 5,519,944 - -0.4 5,519,944 - -1.8 5,519,944 - +0.0 5,519,949 - +0.0 5,519,954 - -0.7 5,520,040 + -0.9 5,520,177 + BWI76_RS26660 0.17 +1.8 5,520,223 - BWI76_RS26660 0.27 -2.5 5,520,366 - BWI76_RS26660 0.57 -3.0 5,520,467 + BWI76_RS26660 0.78 +0.6 5,520,467 + BWI76_RS26660 0.78 +1.0 5,520,509 - BWI76_RS26660 0.87 -1.3 5,520,541 + -0.2 5,520,541 + -0.1 5,520,549 - -0.7 5,520,549 - -0.6 5,520,581 - +0.8 5,520,582 - +3.0 5,520,595 + +1.3 5,520,603 - -0.5 5,520,663 + -0.4 5,520,664 + -1.0 5,520,719 + -0.8 5,520,729 - -1.3 5,520,788 + -0.7 5,520,795 - +0.0 5,520,795 - -1.2 5,520,796 + +0.9 5,520,797 - -2.3 5,520,851 - -2.1 5,520,862 + -1.2 5,520,896 - BWI76_RS26665 0.12 -2.4 5,520,917 + BWI76_RS26665 0.15 -2.5 5,520,953 + BWI76_RS26665 0.20 -3.4 5,521,121 - BWI76_RS26665 0.44 -2.3 5,521,340 + BWI76_RS26665 0.74 -2.3 5,521,512 + -1.7 5,521,520 - -0.9 5,521,573 - -0.7 5,521,585 - -0.6 5,521,595 - -1.2 5,521,621 - -2.0 5,521,646 + +0.7 5,521,654 - -1.7 5,521,666 + BWI76_RS26670 0.11 -1.5 5,521,813 + BWI76_RS26670 0.21 -1.6 5,521,821 - BWI76_RS26670 0.22 -2.2 5,521,821 - BWI76_RS26670 0.22 -4.0 5,521,958 + BWI76_RS26670 0.32 -1.0 5,521,966 - BWI76_RS26670 0.33 -2.5 5,521,966 - BWI76_RS26670 0.33 -1.6 5,522,084 + BWI76_RS26670 0.41 -2.8 5,522,150 + BWI76_RS26670 0.46 -1.1 5,522,177 + BWI76_RS26670 0.48 -1.9 5,522,219 + BWI76_RS26670 0.51 -2.0 5,522,269 - BWI76_RS26670 0.55 -0.8 5,522,349 + BWI76_RS26670 0.61 -1.2 5,522,357 - BWI76_RS26670 0.61 -1.2 5,522,400 - BWI76_RS26670 0.64 -0.8 5,522,421 + BWI76_RS26670 0.66 -1.0 5,522,422 - BWI76_RS26670 0.66 -2.1 5,522,439 - BWI76_RS26670 0.67 -2.1
Or see this region's nucleotide sequence