Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26060 and BWI76_RS26065 are separated by 3 nucleotides BWI76_RS26065 and BWI76_RS26070 overlap by 8 nucleotides BWI76_RS26070 and BWI76_RS26075 are separated by 17 nucleotides
BWI76_RS26060: BWI76_RS26060 - tryptophan--tRNA ligase, at 5,385,429 to 5,386,433
_RS26060
BWI76_RS26065: BWI76_RS26065 - phosphoglycolate phosphatase, at 5,386,437 to 5,387,186
_RS26065
BWI76_RS26070: BWI76_RS26070 - ribulose-phosphate 3-epimerase, at 5,387,179 to 5,387,856
_RS26070
BWI76_RS26075: BWI76_RS26075 - DNA adenine methylase, at 5,387,874 to 5,388,701
_RS26075
Position (kb) 5386
5387
5388 Strain fitness (log2 ratio) -2
-1
0
1 at 5385.440 kb on - strand at 5385.542 kb on - strand, within BWI76_RS26060 at 5385.542 kb on - strand, within BWI76_RS26060 at 5385.564 kb on + strand, within BWI76_RS26060 at 5385.610 kb on + strand, within BWI76_RS26060 at 5385.706 kb on - strand, within BWI76_RS26060 at 5385.867 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.190 kb on + strand, within BWI76_RS26060 at 5386.236 kb on - strand, within BWI76_RS26060 at 5386.258 kb on + strand, within BWI76_RS26060 at 5386.266 kb on - strand, within BWI76_RS26060 at 5386.266 kb on - strand, within BWI76_RS26060 at 5386.393 kb on + strand at 5386.393 kb on + strand at 5386.401 kb on - strand at 5386.542 kb on - strand, within BWI76_RS26065 at 5386.657 kb on + strand, within BWI76_RS26065 at 5386.746 kb on + strand, within BWI76_RS26065 at 5386.993 kb on + strand, within BWI76_RS26065 at 5387.143 kb on - strand at 5387.194 kb on - strand at 5387.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,385,440 - -0.6 5,385,542 - BWI76_RS26060 0.11 -0.8 5,385,542 - BWI76_RS26060 0.11 -0.4 5,385,564 + BWI76_RS26060 0.13 -0.9 5,385,610 + BWI76_RS26060 0.18 -1.3 5,385,706 - BWI76_RS26060 0.28 -0.1 5,385,867 - BWI76_RS26060 0.44 -0.6 5,386,074 - BWI76_RS26060 0.64 +0.2 5,386,074 - BWI76_RS26060 0.64 -2.4 5,386,074 - BWI76_RS26060 0.64 -0.9 5,386,190 + BWI76_RS26060 0.76 +0.2 5,386,236 - BWI76_RS26060 0.80 -1.5 5,386,258 + BWI76_RS26060 0.82 -0.8 5,386,266 - BWI76_RS26060 0.83 -1.8 5,386,266 - BWI76_RS26060 0.83 -0.0 5,386,393 + +0.4 5,386,393 + -0.1 5,386,401 - +1.4 5,386,542 - BWI76_RS26065 0.14 -0.5 5,386,657 + BWI76_RS26065 0.29 -2.3 5,386,746 + BWI76_RS26065 0.41 -1.7 5,386,993 + BWI76_RS26065 0.74 -1.8 5,387,143 - +1.1 5,387,194 - -1.3 5,387,903 - -0.8
Or see this region's nucleotide sequence