Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25450 and BWI76_RS25455 are separated by 7 nucleotides BWI76_RS25455 and BWI76_RS25460 are separated by 131 nucleotides BWI76_RS25460 and BWI76_RS25465 overlap by 4 nucleotides BWI76_RS25465 and BWI76_RS25470 are separated by 58 nucleotides
BWI76_RS25450: BWI76_RS25450 - p-hydroxybenzoic acid efflux subunit AaeA, at 5,288,482 to 5,289,414
_RS25450
BWI76_RS25455: BWI76_RS25455 - protein AaeX, at 5,289,422 to 5,289,625
_RS25455
BWI76_RS25460: BWI76_RS25460 - transcriptional regulator, at 5,289,757 to 5,290,686
_RS25460
BWI76_RS25465: BWI76_RS25465 - transcriptional regulator, at 5,290,683 to 5,291,429
_RS25465
BWI76_RS25470: BWI76_RS25470 - metalloprotease TldD, at 5,291,488 to 5,292,933
_RS25470
Position (kb)
5289
5290
5291 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5288.804 kb on + strand, within BWI76_RS25450 at 5288.828 kb on - strand, within BWI76_RS25450 at 5289.471 kb on + strand, within BWI76_RS25455 at 5289.471 kb on + strand, within BWI76_RS25455 at 5289.471 kb on + strand, within BWI76_RS25455 at 5289.479 kb on - strand, within BWI76_RS25455 at 5289.479 kb on - strand, within BWI76_RS25455 at 5289.479 kb on - strand, within BWI76_RS25455 at 5289.479 kb on - strand, within BWI76_RS25455 at 5289.479 kb on - strand, within BWI76_RS25455 at 5289.479 kb on - strand, within BWI76_RS25455 at 5289.489 kb on - strand, within BWI76_RS25455 at 5289.623 kb on + strand at 5289.920 kb on + strand, within BWI76_RS25460 at 5289.928 kb on - strand, within BWI76_RS25460 at 5289.952 kb on + strand, within BWI76_RS25460 at 5290.002 kb on + strand, within BWI76_RS25460 at 5290.009 kb on - strand, within BWI76_RS25460 at 5290.109 kb on + strand, within BWI76_RS25460 at 5290.168 kb on + strand, within BWI76_RS25460 at 5290.169 kb on + strand, within BWI76_RS25460 at 5290.396 kb on + strand, within BWI76_RS25460 at 5290.396 kb on - strand, within BWI76_RS25460 at 5290.404 kb on - strand, within BWI76_RS25460 at 5290.456 kb on - strand, within BWI76_RS25460 at 5290.482 kb on - strand, within BWI76_RS25460 at 5290.603 kb on - strand at 5290.656 kb on + strand at 5290.697 kb on - strand at 5290.834 kb on + strand at 5290.966 kb on + strand, within BWI76_RS25465 at 5290.974 kb on - strand, within BWI76_RS25465 at 5290.995 kb on + strand, within BWI76_RS25465 at 5291.042 kb on + strand, within BWI76_RS25465 at 5291.283 kb on - strand, within BWI76_RS25465 at 5291.290 kb on - strand, within BWI76_RS25465 at 5291.348 kb on - strand, within BWI76_RS25465 at 5291.485 kb on + strand at 5291.656 kb on + strand, within BWI76_RS25470 at 5291.663 kb on - strand, within BWI76_RS25470
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,288,804 + BWI76_RS25450 0.35 -0.7 5,288,828 - BWI76_RS25450 0.37 -0.0 5,289,471 + BWI76_RS25455 0.24 -0.1 5,289,471 + BWI76_RS25455 0.24 +0.6 5,289,471 + BWI76_RS25455 0.24 +0.2 5,289,479 - BWI76_RS25455 0.28 +1.8 5,289,479 - BWI76_RS25455 0.28 -0.4 5,289,479 - BWI76_RS25455 0.28 +0.9 5,289,479 - BWI76_RS25455 0.28 -0.1 5,289,479 - BWI76_RS25455 0.28 -0.5 5,289,479 - BWI76_RS25455 0.28 -1.5 5,289,489 - BWI76_RS25455 0.33 -1.0 5,289,623 + -1.4 5,289,920 + BWI76_RS25460 0.18 +0.6 5,289,928 - BWI76_RS25460 0.18 -0.4 5,289,952 + BWI76_RS25460 0.21 -0.9 5,290,002 + BWI76_RS25460 0.26 +0.1 5,290,009 - BWI76_RS25460 0.27 -0.3 5,290,109 + BWI76_RS25460 0.38 -0.6 5,290,168 + BWI76_RS25460 0.44 -3.1 5,290,169 + BWI76_RS25460 0.44 -0.5 5,290,396 + BWI76_RS25460 0.69 -1.2 5,290,396 - BWI76_RS25460 0.69 -1.6 5,290,404 - BWI76_RS25460 0.70 -0.9 5,290,456 - BWI76_RS25460 0.75 +0.6 5,290,482 - BWI76_RS25460 0.78 -0.1 5,290,603 - -0.4 5,290,656 + -1.0 5,290,697 - -1.0 5,290,834 + +2.0 5,290,966 + BWI76_RS25465 0.38 -1.1 5,290,974 - BWI76_RS25465 0.39 -0.7 5,290,995 + BWI76_RS25465 0.42 -0.8 5,291,042 + BWI76_RS25465 0.48 -0.3 5,291,283 - BWI76_RS25465 0.80 -0.1 5,291,290 - BWI76_RS25465 0.81 -0.9 5,291,348 - BWI76_RS25465 0.89 -0.8 5,291,485 + -0.3 5,291,656 + BWI76_RS25470 0.12 +0.4 5,291,663 - BWI76_RS25470 0.12 +0.5
Or see this region's nucleotide sequence