Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25460

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25450 and BWI76_RS25455 are separated by 7 nucleotidesBWI76_RS25455 and BWI76_RS25460 are separated by 131 nucleotidesBWI76_RS25460 and BWI76_RS25465 overlap by 4 nucleotidesBWI76_RS25465 and BWI76_RS25470 are separated by 58 nucleotides BWI76_RS25450: BWI76_RS25450 - p-hydroxybenzoic acid efflux subunit AaeA, at 5,288,482 to 5,289,414 _RS25450 BWI76_RS25455: BWI76_RS25455 - protein AaeX, at 5,289,422 to 5,289,625 _RS25455 BWI76_RS25460: BWI76_RS25460 - transcriptional regulator, at 5,289,757 to 5,290,686 _RS25460 BWI76_RS25465: BWI76_RS25465 - transcriptional regulator, at 5,290,683 to 5,291,429 _RS25465 BWI76_RS25470: BWI76_RS25470 - metalloprotease TldD, at 5,291,488 to 5,292,933 _RS25470 Position (kb) 5289 5290 5291Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5288.804 kb on + strand, within BWI76_RS25450at 5288.828 kb on - strand, within BWI76_RS25450at 5289.471 kb on + strand, within BWI76_RS25455at 5289.471 kb on + strand, within BWI76_RS25455at 5289.471 kb on + strand, within BWI76_RS25455at 5289.479 kb on - strand, within BWI76_RS25455at 5289.479 kb on - strand, within BWI76_RS25455at 5289.479 kb on - strand, within BWI76_RS25455at 5289.479 kb on - strand, within BWI76_RS25455at 5289.479 kb on - strand, within BWI76_RS25455at 5289.479 kb on - strand, within BWI76_RS25455at 5289.489 kb on - strand, within BWI76_RS25455at 5289.623 kb on + strandat 5289.920 kb on + strand, within BWI76_RS25460at 5289.928 kb on - strand, within BWI76_RS25460at 5289.952 kb on + strand, within BWI76_RS25460at 5290.002 kb on + strand, within BWI76_RS25460at 5290.009 kb on - strand, within BWI76_RS25460at 5290.109 kb on + strand, within BWI76_RS25460at 5290.168 kb on + strand, within BWI76_RS25460at 5290.169 kb on + strand, within BWI76_RS25460at 5290.396 kb on + strand, within BWI76_RS25460at 5290.396 kb on - strand, within BWI76_RS25460at 5290.404 kb on - strand, within BWI76_RS25460at 5290.456 kb on - strand, within BWI76_RS25460at 5290.482 kb on - strand, within BWI76_RS25460at 5290.603 kb on - strandat 5290.656 kb on + strandat 5290.697 kb on - strandat 5290.834 kb on + strandat 5290.966 kb on + strand, within BWI76_RS25465at 5290.974 kb on - strand, within BWI76_RS25465at 5290.995 kb on + strand, within BWI76_RS25465at 5291.042 kb on + strand, within BWI76_RS25465at 5291.283 kb on - strand, within BWI76_RS25465at 5291.290 kb on - strand, within BWI76_RS25465at 5291.348 kb on - strand, within BWI76_RS25465at 5291.485 kb on + strandat 5291.656 kb on + strand, within BWI76_RS25470at 5291.663 kb on - strand, within BWI76_RS25470

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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5,288,804 + BWI76_RS25450 0.35 -0.7
5,288,828 - BWI76_RS25450 0.37 -0.0
5,289,471 + BWI76_RS25455 0.24 -0.1
5,289,471 + BWI76_RS25455 0.24 +0.6
5,289,471 + BWI76_RS25455 0.24 +0.2
5,289,479 - BWI76_RS25455 0.28 +1.8
5,289,479 - BWI76_RS25455 0.28 -0.4
5,289,479 - BWI76_RS25455 0.28 +0.9
5,289,479 - BWI76_RS25455 0.28 -0.1
5,289,479 - BWI76_RS25455 0.28 -0.5
5,289,479 - BWI76_RS25455 0.28 -1.5
5,289,489 - BWI76_RS25455 0.33 -1.0
5,289,623 + -1.4
5,289,920 + BWI76_RS25460 0.18 +0.6
5,289,928 - BWI76_RS25460 0.18 -0.4
5,289,952 + BWI76_RS25460 0.21 -0.9
5,290,002 + BWI76_RS25460 0.26 +0.1
5,290,009 - BWI76_RS25460 0.27 -0.3
5,290,109 + BWI76_RS25460 0.38 -0.6
5,290,168 + BWI76_RS25460 0.44 -3.1
5,290,169 + BWI76_RS25460 0.44 -0.5
5,290,396 + BWI76_RS25460 0.69 -1.2
5,290,396 - BWI76_RS25460 0.69 -1.6
5,290,404 - BWI76_RS25460 0.70 -0.9
5,290,456 - BWI76_RS25460 0.75 +0.6
5,290,482 - BWI76_RS25460 0.78 -0.1
5,290,603 - -0.4
5,290,656 + -1.0
5,290,697 - -1.0
5,290,834 + +2.0
5,290,966 + BWI76_RS25465 0.38 -1.1
5,290,974 - BWI76_RS25465 0.39 -0.7
5,290,995 + BWI76_RS25465 0.42 -0.8
5,291,042 + BWI76_RS25465 0.48 -0.3
5,291,283 - BWI76_RS25465 0.80 -0.1
5,291,290 - BWI76_RS25465 0.81 -0.9
5,291,348 - BWI76_RS25465 0.89 -0.8
5,291,485 + -0.3
5,291,656 + BWI76_RS25470 0.12 +0.4
5,291,663 - BWI76_RS25470 0.12 +0.5

Or see this region's nucleotide sequence