Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25300

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25290 and BWI76_RS25295 overlap by 4 nucleotidesBWI76_RS25295 and BWI76_RS25300 are separated by 234 nucleotidesBWI76_RS25300 and BWI76_RS25305 are separated by 142 nucleotides BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092 _RS25290 BWI76_RS25295: BWI76_RS25295 - glutamine amidotransferase, at 5,256,089 to 5,256,742 _RS25295 BWI76_RS25300: BWI76_RS25300 - hybrid sensor histidine kinase/response regulator, at 5,256,977 to 5,259,316 _RS25300 BWI76_RS25305: BWI76_RS25305 - TIGR01212 family radical SAM protein, at 5,259,459 to 5,260,394 _RS25305 Position (kb) 5256 5257 5258 5259 5260Strain fitness (log2 ratio) -3 -2 -1 0 1at 5255.989 kb on + strand, within BWI76_RS25290at 5255.989 kb on + strand, within BWI76_RS25290at 5255.994 kb on + strand, within BWI76_RS25290at 5255.997 kb on - strand, within BWI76_RS25290at 5256.013 kb on + strand, within BWI76_RS25290at 5256.021 kb on - strandat 5256.347 kb on + strand, within BWI76_RS25295at 5256.468 kb on + strand, within BWI76_RS25295at 5256.521 kb on - strand, within BWI76_RS25295at 5256.556 kb on + strand, within BWI76_RS25295at 5256.724 kb on - strandat 5256.729 kb on - strandat 5256.745 kb on + strandat 5256.960 kb on + strandat 5257.183 kb on - strandat 5257.207 kb on - strandat 5257.453 kb on - strand, within BWI76_RS25300at 5257.781 kb on - strand, within BWI76_RS25300at 5258.587 kb on + strand, within BWI76_RS25300at 5258.761 kb on + strand, within BWI76_RS25300at 5258.990 kb on - strand, within BWI76_RS25300at 5259.039 kb on - strand, within BWI76_RS25300at 5259.105 kb on - strandat 5259.170 kb on - strandat 5259.189 kb on - strandat 5259.282 kb on + strandat 5259.290 kb on - strandat 5259.290 kb on - strandat 5259.416 kb on + strandat 5259.439 kb on + strandat 5259.512 kb on - strandat 5259.568 kb on + strand, within BWI76_RS25305at 5259.571 kb on - strand, within BWI76_RS25305at 5259.718 kb on - strand, within BWI76_RS25305at 5259.758 kb on + strand, within BWI76_RS25305at 5259.766 kb on - strand, within BWI76_RS25305at 5259.937 kb on + strand, within BWI76_RS25305at 5259.969 kb on + strand, within BWI76_RS25305at 5259.985 kb on + strand, within BWI76_RS25305

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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5,255,989 + BWI76_RS25290 0.86 -0.4
5,255,989 + BWI76_RS25290 0.86 -0.6
5,255,994 + BWI76_RS25290 0.86 -2.0
5,255,997 - BWI76_RS25290 0.87 -0.1
5,256,013 + BWI76_RS25290 0.89 +0.9
5,256,021 - +0.3
5,256,347 + BWI76_RS25295 0.39 -1.3
5,256,468 + BWI76_RS25295 0.58 -0.1
5,256,521 - BWI76_RS25295 0.66 -0.6
5,256,556 + BWI76_RS25295 0.71 +0.1
5,256,724 - -1.6
5,256,729 - +0.5
5,256,745 + +0.1
5,256,960 + -0.3
5,257,183 - -0.0
5,257,207 - -0.9
5,257,453 - BWI76_RS25300 0.20 -1.0
5,257,781 - BWI76_RS25300 0.34 -2.3
5,258,587 + BWI76_RS25300 0.69 -2.2
5,258,761 + BWI76_RS25300 0.76 -0.5
5,258,990 - BWI76_RS25300 0.86 -1.0
5,259,039 - BWI76_RS25300 0.88 -2.5
5,259,105 - -3.1
5,259,170 - +1.0
5,259,189 - -1.6
5,259,282 + +0.0
5,259,290 - +0.1
5,259,290 - -1.4
5,259,416 + -1.5
5,259,439 + -0.8
5,259,512 - +1.0
5,259,568 + BWI76_RS25305 0.12 -2.5
5,259,571 - BWI76_RS25305 0.12 -1.3
5,259,718 - BWI76_RS25305 0.28 +1.4
5,259,758 + BWI76_RS25305 0.32 -0.6
5,259,766 - BWI76_RS25305 0.33 +0.8
5,259,937 + BWI76_RS25305 0.51 -0.6
5,259,969 + BWI76_RS25305 0.54 +1.2
5,259,985 + BWI76_RS25305 0.56 -1.4

Or see this region's nucleotide sequence