Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25290 and BWI76_RS25295 overlap by 4 nucleotides BWI76_RS25295 and BWI76_RS25300 are separated by 234 nucleotides BWI76_RS25300 and BWI76_RS25305 are separated by 142 nucleotides
BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092
_RS25290
BWI76_RS25295: BWI76_RS25295 - glutamine amidotransferase, at 5,256,089 to 5,256,742
_RS25295
BWI76_RS25300: BWI76_RS25300 - hybrid sensor histidine kinase/response regulator, at 5,256,977 to 5,259,316
_RS25300
BWI76_RS25305: BWI76_RS25305 - TIGR01212 family radical SAM protein, at 5,259,459 to 5,260,394
_RS25305
Position (kb) 5256
5257
5258
5259
5260 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.994 kb on + strand, within BWI76_RS25290 at 5255.997 kb on - strand, within BWI76_RS25290 at 5256.013 kb on + strand, within BWI76_RS25290 at 5256.021 kb on - strand at 5256.347 kb on + strand, within BWI76_RS25295 at 5256.468 kb on + strand, within BWI76_RS25295 at 5256.521 kb on - strand, within BWI76_RS25295 at 5256.556 kb on + strand, within BWI76_RS25295 at 5256.724 kb on - strand at 5256.729 kb on - strand at 5256.745 kb on + strand at 5256.960 kb on + strand at 5257.183 kb on - strand at 5257.207 kb on - strand at 5257.453 kb on - strand, within BWI76_RS25300 at 5257.781 kb on - strand, within BWI76_RS25300 at 5258.587 kb on + strand, within BWI76_RS25300 at 5258.761 kb on + strand, within BWI76_RS25300 at 5258.990 kb on - strand, within BWI76_RS25300 at 5259.039 kb on - strand, within BWI76_RS25300 at 5259.105 kb on - strand at 5259.170 kb on - strand at 5259.189 kb on - strand at 5259.282 kb on + strand at 5259.290 kb on - strand at 5259.290 kb on - strand at 5259.416 kb on + strand at 5259.439 kb on + strand at 5259.512 kb on - strand at 5259.568 kb on + strand, within BWI76_RS25305 at 5259.571 kb on - strand, within BWI76_RS25305 at 5259.718 kb on - strand, within BWI76_RS25305 at 5259.758 kb on + strand, within BWI76_RS25305 at 5259.766 kb on - strand, within BWI76_RS25305 at 5259.937 kb on + strand, within BWI76_RS25305 at 5259.969 kb on + strand, within BWI76_RS25305 at 5259.985 kb on + strand, within BWI76_RS25305
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,255,989 + BWI76_RS25290 0.86 -0.4 5,255,989 + BWI76_RS25290 0.86 -0.6 5,255,994 + BWI76_RS25290 0.86 -2.0 5,255,997 - BWI76_RS25290 0.87 -0.1 5,256,013 + BWI76_RS25290 0.89 +0.9 5,256,021 - +0.3 5,256,347 + BWI76_RS25295 0.39 -1.3 5,256,468 + BWI76_RS25295 0.58 -0.1 5,256,521 - BWI76_RS25295 0.66 -0.6 5,256,556 + BWI76_RS25295 0.71 +0.1 5,256,724 - -1.6 5,256,729 - +0.5 5,256,745 + +0.1 5,256,960 + -0.3 5,257,183 - -0.0 5,257,207 - -0.9 5,257,453 - BWI76_RS25300 0.20 -1.0 5,257,781 - BWI76_RS25300 0.34 -2.3 5,258,587 + BWI76_RS25300 0.69 -2.2 5,258,761 + BWI76_RS25300 0.76 -0.5 5,258,990 - BWI76_RS25300 0.86 -1.0 5,259,039 - BWI76_RS25300 0.88 -2.5 5,259,105 - -3.1 5,259,170 - +1.0 5,259,189 - -1.6 5,259,282 + +0.0 5,259,290 - +0.1 5,259,290 - -1.4 5,259,416 + -1.5 5,259,439 + -0.8 5,259,512 - +1.0 5,259,568 + BWI76_RS25305 0.12 -2.5 5,259,571 - BWI76_RS25305 0.12 -1.3 5,259,718 - BWI76_RS25305 0.28 +1.4 5,259,758 + BWI76_RS25305 0.32 -0.6 5,259,766 - BWI76_RS25305 0.33 +0.8 5,259,937 + BWI76_RS25305 0.51 -0.6 5,259,969 + BWI76_RS25305 0.54 +1.2 5,259,985 + BWI76_RS25305 0.56 -1.4
Or see this region's nucleotide sequence