Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25190 and BWI76_RS25195 are separated by 198 nucleotides BWI76_RS25195 and BWI76_RS25200 are separated by 56 nucleotides BWI76_RS25200 and BWI76_RS25205 are separated by 54 nucleotides BWI76_RS25205 and BWI76_RS25210 are separated by 135 nucleotides BWI76_RS25210 and BWI76_RS25215 overlap by 1 nucleotides
BWI76_RS25190: BWI76_RS25190 - octaprenyl-diphosphate synthase, at 5,240,907 to 5,241,878
_RS25190
BWI76_RS25195: BWI76_RS25195 - maltose metabolism regulator, at 5,242,077 to 5,242,349
_RS25195
BWI76_RS25200: BWI76_RS25200 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 5,242,406 to 5,243,665
_RS25200
BWI76_RS25205: BWI76_RS25205 - hypothetical protein, at 5,243,720 to 5,243,974
_RS25205
BWI76_RS25210: BWI76_RS25210 - sulfate transporter, at 5,244,110 to 5,244,400
_RS25210
BWI76_RS25215: BWI76_RS25215 - phospholipid-binding protein MlaC, at 5,244,400 to 5,245,035
_RS25215
Position (kb) 5242
5243
5244 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 5241.881 kb on - strand at 5242.065 kb on - strand at 5242.205 kb on + strand, within BWI76_RS25195 at 5242.213 kb on - strand, within BWI76_RS25195 at 5242.354 kb on + strand at 5242.371 kb on + strand at 5243.761 kb on - strand, within BWI76_RS25205 at 5244.001 kb on - strand at 5244.019 kb on - strand at 5244.039 kb on - strand at 5244.176 kb on + strand, within BWI76_RS25210 at 5244.381 kb on + strand at 5244.440 kb on + strand at 5244.624 kb on + strand, within BWI76_RS25215
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,241,881 - +0.7 5,242,065 - -0.9 5,242,205 + BWI76_RS25195 0.47 +4.9 5,242,213 - BWI76_RS25195 0.50 -0.1 5,242,354 + +1.0 5,242,371 + -1.4 5,243,761 - BWI76_RS25205 0.16 +0.1 5,244,001 - -0.7 5,244,019 - -1.3 5,244,039 - -0.9 5,244,176 + BWI76_RS25210 0.23 +0.3 5,244,381 + -0.4 5,244,440 + -0.0 5,244,624 + BWI76_RS25215 0.35 -2.2
Or see this region's nucleotide sequence