Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24675 and BWI76_RS24680 are separated by 74 nucleotides BWI76_RS24680 and BWI76_RS24685 are separated by 104 nucleotides
BWI76_RS24675: BWI76_RS24675 - NADPH-dependent 2,4-dienoyl-CoA reductase, at 5,143,554 to 5,145,575
_RS24675
BWI76_RS24680: BWI76_RS24680 - 23S rRNA (guanine(1835)-N(2))-methyltransferase, at 5,145,650 to 5,146,780
_RS24680
BWI76_RS24685: BWI76_RS24685 - MFS transporter, at 5,146,885 to 5,148,228
_RS24685
Position (kb)
5145
5146
5147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5144.725 kb on - strand, within BWI76_RS24675 at 5144.754 kb on - strand, within BWI76_RS24675 at 5144.762 kb on + strand, within BWI76_RS24675 at 5145.102 kb on - strand, within BWI76_RS24675 at 5145.104 kb on + strand, within BWI76_RS24675 at 5145.137 kb on + strand, within BWI76_RS24675 at 5145.137 kb on + strand, within BWI76_RS24675 at 5145.173 kb on - strand, within BWI76_RS24675 at 5145.226 kb on + strand, within BWI76_RS24675 at 5145.391 kb on + strand at 5145.484 kb on - strand at 5145.641 kb on + strand at 5145.656 kb on - strand at 5145.667 kb on + strand at 5145.675 kb on + strand at 5145.999 kb on + strand, within BWI76_RS24680 at 5146.026 kb on - strand, within BWI76_RS24680 at 5146.079 kb on + strand, within BWI76_RS24680 at 5146.148 kb on - strand, within BWI76_RS24680 at 5146.168 kb on - strand, within BWI76_RS24680 at 5146.186 kb on - strand, within BWI76_RS24680 at 5146.568 kb on - strand, within BWI76_RS24680 at 5146.573 kb on + strand, within BWI76_RS24680 at 5146.738 kb on + strand at 5146.738 kb on + strand at 5146.760 kb on + strand at 5146.760 kb on + strand at 5146.837 kb on + strand at 5146.837 kb on + strand at 5146.840 kb on + strand at 5146.848 kb on - strand at 5146.848 kb on - strand at 5146.882 kb on + strand at 5146.908 kb on - strand at 5147.198 kb on + strand, within BWI76_RS24685 at 5147.201 kb on - strand, within BWI76_RS24685 at 5147.293 kb on - strand, within BWI76_RS24685 at 5147.294 kb on + strand, within BWI76_RS24685 at 5147.294 kb on + strand, within BWI76_RS24685 at 5147.294 kb on + strand, within BWI76_RS24685 at 5147.302 kb on - strand, within BWI76_RS24685 at 5147.302 kb on - strand, within BWI76_RS24685 at 5147.393 kb on + strand, within BWI76_RS24685 at 5147.411 kb on - strand, within BWI76_RS24685 at 5147.433 kb on - strand, within BWI76_RS24685 at 5147.477 kb on + strand, within BWI76_RS24685 at 5147.477 kb on + strand, within BWI76_RS24685 at 5147.477 kb on + strand, within BWI76_RS24685 at 5147.477 kb on + strand, within BWI76_RS24685 at 5147.551 kb on + strand, within BWI76_RS24685 at 5147.575 kb on + strand, within BWI76_RS24685 at 5147.583 kb on - strand, within BWI76_RS24685 at 5147.589 kb on - strand, within BWI76_RS24685 at 5147.597 kb on + strand, within BWI76_RS24685 at 5147.622 kb on + strand, within BWI76_RS24685 at 5147.622 kb on + strand, within BWI76_RS24685 at 5147.701 kb on + strand, within BWI76_RS24685 at 5147.701 kb on + strand, within BWI76_RS24685 at 5147.704 kb on - strand, within BWI76_RS24685 at 5147.709 kb on - strand, within BWI76_RS24685 at 5147.709 kb on - strand, within BWI76_RS24685 at 5147.709 kb on - strand, within BWI76_RS24685 at 5147.709 kb on - strand, within BWI76_RS24685 at 5147.709 kb on - strand, within BWI76_RS24685 at 5147.725 kb on + strand, within BWI76_RS24685 at 5147.728 kb on - strand, within BWI76_RS24685 at 5147.759 kb on + strand, within BWI76_RS24685 at 5147.759 kb on + strand, within BWI76_RS24685 at 5147.767 kb on - strand, within BWI76_RS24685 at 5147.767 kb on - strand, within BWI76_RS24685 at 5147.767 kb on - strand, within BWI76_RS24685
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,144,725 - BWI76_RS24675 0.58 -0.9 5,144,754 - BWI76_RS24675 0.59 +0.0 5,144,762 + BWI76_RS24675 0.60 -0.1 5,145,102 - BWI76_RS24675 0.77 +0.4 5,145,104 + BWI76_RS24675 0.77 -0.0 5,145,137 + BWI76_RS24675 0.78 -0.2 5,145,137 + BWI76_RS24675 0.78 +0.6 5,145,173 - BWI76_RS24675 0.80 +0.0 5,145,226 + BWI76_RS24675 0.83 +2.1 5,145,391 + -0.3 5,145,484 - +5.0 5,145,641 + -1.3 5,145,656 - -1.3 5,145,667 + -1.0 5,145,675 + -1.6 5,145,999 + BWI76_RS24680 0.31 +2.2 5,146,026 - BWI76_RS24680 0.33 +2.1 5,146,079 + BWI76_RS24680 0.38 +3.7 5,146,148 - BWI76_RS24680 0.44 +0.9 5,146,168 - BWI76_RS24680 0.46 +0.3 5,146,186 - BWI76_RS24680 0.47 +0.1 5,146,568 - BWI76_RS24680 0.81 -0.7 5,146,573 + BWI76_RS24680 0.82 -3.3 5,146,738 + +2.1 5,146,738 + +0.2 5,146,760 + +1.7 5,146,760 + -0.2 5,146,837 + +0.6 5,146,837 + -0.8 5,146,840 + -1.1 5,146,848 - -2.0 5,146,848 - -1.0 5,146,882 + -1.0 5,146,908 - +0.4 5,147,198 + BWI76_RS24685 0.23 +0.4 5,147,201 - BWI76_RS24685 0.24 -0.1 5,147,293 - BWI76_RS24685 0.30 +0.4 5,147,294 + BWI76_RS24685 0.30 +0.1 5,147,294 + BWI76_RS24685 0.30 -0.6 5,147,294 + BWI76_RS24685 0.30 -0.2 5,147,302 - BWI76_RS24685 0.31 +1.0 5,147,302 - BWI76_RS24685 0.31 -0.2 5,147,393 + BWI76_RS24685 0.38 +0.7 5,147,411 - BWI76_RS24685 0.39 -1.3 5,147,433 - BWI76_RS24685 0.41 +0.4 5,147,477 + BWI76_RS24685 0.44 -0.1 5,147,477 + BWI76_RS24685 0.44 +0.5 5,147,477 + BWI76_RS24685 0.44 -1.3 5,147,477 + BWI76_RS24685 0.44 -0.3 5,147,551 + BWI76_RS24685 0.50 -1.0 5,147,575 + BWI76_RS24685 0.51 -1.1 5,147,583 - BWI76_RS24685 0.52 -1.4 5,147,589 - BWI76_RS24685 0.52 +0.5 5,147,597 + BWI76_RS24685 0.53 -1.1 5,147,622 + BWI76_RS24685 0.55 +1.3 5,147,622 + BWI76_RS24685 0.55 -2.4 5,147,701 + BWI76_RS24685 0.61 -0.4 5,147,701 + BWI76_RS24685 0.61 +0.3 5,147,704 - BWI76_RS24685 0.61 +0.6 5,147,709 - BWI76_RS24685 0.61 -0.8 5,147,709 - BWI76_RS24685 0.61 -0.1 5,147,709 - BWI76_RS24685 0.61 +0.2 5,147,709 - BWI76_RS24685 0.61 -1.8 5,147,709 - BWI76_RS24685 0.61 -0.2 5,147,725 + BWI76_RS24685 0.62 +1.0 5,147,728 - BWI76_RS24685 0.63 -2.1 5,147,759 + BWI76_RS24685 0.65 +0.4 5,147,759 + BWI76_RS24685 0.65 +1.4 5,147,767 - BWI76_RS24685 0.66 -0.7 5,147,767 - BWI76_RS24685 0.66 -0.7 5,147,767 - BWI76_RS24685 0.66 +1.9
Or see this region's nucleotide sequence