Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22860

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22850 and BWI76_RS22855 overlap by 1 nucleotidesBWI76_RS22855 and BWI76_RS22860 are separated by 633 nucleotidesBWI76_RS22860 and BWI76_RS22865 are separated by 69 nucleotides BWI76_RS22850: BWI76_RS22850 - tRNA pseudouridine synthase TruC, at 4,767,545 to 4,768,339 _RS22850 BWI76_RS22855: BWI76_RS22855 - hypothetical protein, at 4,768,339 to 4,768,668 _RS22855 BWI76_RS22860: BWI76_RS22860 - SecY-interacting protein, at 4,769,302 to 4,769,847 _RS22860 BWI76_RS22865: BWI76_RS22865 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 4,769,917 to 4,770,762 _RS22865 Position (kb) 4769 4770Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5 6 7at 4768.370 kb on + strandat 4768.428 kb on + strand, within BWI76_RS22855at 4768.530 kb on + strand, within BWI76_RS22855at 4768.538 kb on - strand, within BWI76_RS22855at 4768.610 kb on - strand, within BWI76_RS22855at 4768.748 kb on - strandat 4768.748 kb on - strandat 4768.803 kb on - strandat 4768.859 kb on - strandat 4768.902 kb on - strandat 4768.904 kb on + strandat 4768.985 kb on - strandat 4769.116 kb on + strandat 4769.378 kb on - strand, within BWI76_RS22860at 4769.683 kb on - strand, within BWI76_RS22860at 4769.798 kb on + strandat 4769.826 kb on + strandat 4769.834 kb on - strandat 4769.880 kb on - strandat 4769.948 kb on - strandat 4770.001 kb on - strandat 4770.003 kb on + strand, within BWI76_RS22865at 4770.011 kb on - strand, within BWI76_RS22865at 4770.061 kb on - strand, within BWI76_RS22865at 4770.091 kb on + strand, within BWI76_RS22865at 4770.099 kb on + strand, within BWI76_RS22865at 4770.399 kb on + strand, within BWI76_RS22865at 4770.415 kb on - strand, within BWI76_RS22865at 4770.442 kb on + strand, within BWI76_RS22865at 4770.830 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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4,768,370 + -0.4
4,768,428 + BWI76_RS22855 0.27 -1.9
4,768,530 + BWI76_RS22855 0.58 -0.5
4,768,538 - BWI76_RS22855 0.60 -0.2
4,768,610 - BWI76_RS22855 0.82 +0.5
4,768,748 - +6.9
4,768,748 - -0.9
4,768,803 - +0.6
4,768,859 - +1.3
4,768,902 - -3.8
4,768,904 + +0.4
4,768,985 - +0.3
4,769,116 + -0.2
4,769,378 - BWI76_RS22860 0.14 +1.2
4,769,683 - BWI76_RS22860 0.70 +1.4
4,769,798 + +0.2
4,769,826 + -1.2
4,769,834 - -1.4
4,769,880 - +0.2
4,769,948 - -2.8
4,770,001 - -0.5
4,770,003 + BWI76_RS22865 0.10 -0.8
4,770,011 - BWI76_RS22865 0.11 +1.1
4,770,061 - BWI76_RS22865 0.17 -0.6
4,770,091 + BWI76_RS22865 0.21 -0.4
4,770,099 + BWI76_RS22865 0.22 +0.3
4,770,399 + BWI76_RS22865 0.57 -0.1
4,770,415 - BWI76_RS22865 0.59 -2.6
4,770,442 + BWI76_RS22865 0.62 +1.8
4,770,830 + -3.3

Or see this region's nucleotide sequence