Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22850 and BWI76_RS22855 overlap by 1 nucleotides BWI76_RS22855 and BWI76_RS22860 are separated by 633 nucleotides BWI76_RS22860 and BWI76_RS22865 are separated by 69 nucleotides
BWI76_RS22850: BWI76_RS22850 - tRNA pseudouridine synthase TruC, at 4,767,545 to 4,768,339
_RS22850
BWI76_RS22855: BWI76_RS22855 - hypothetical protein, at 4,768,339 to 4,768,668
_RS22855
BWI76_RS22860: BWI76_RS22860 - SecY-interacting protein, at 4,769,302 to 4,769,847
_RS22860
BWI76_RS22865: BWI76_RS22865 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 4,769,917 to 4,770,762
_RS22865
Position (kb) 4769
4770 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7 at 4768.370 kb on + strand at 4768.428 kb on + strand, within BWI76_RS22855 at 4768.530 kb on + strand, within BWI76_RS22855 at 4768.538 kb on - strand, within BWI76_RS22855 at 4768.610 kb on - strand, within BWI76_RS22855 at 4768.748 kb on - strand at 4768.748 kb on - strand at 4768.803 kb on - strand at 4768.859 kb on - strand at 4768.902 kb on - strand at 4768.904 kb on + strand at 4768.985 kb on - strand at 4769.116 kb on + strand at 4769.378 kb on - strand, within BWI76_RS22860 at 4769.683 kb on - strand, within BWI76_RS22860 at 4769.798 kb on + strand at 4769.826 kb on + strand at 4769.834 kb on - strand at 4769.880 kb on - strand at 4769.948 kb on - strand at 4770.001 kb on - strand at 4770.003 kb on + strand, within BWI76_RS22865 at 4770.011 kb on - strand, within BWI76_RS22865 at 4770.061 kb on - strand, within BWI76_RS22865 at 4770.091 kb on + strand, within BWI76_RS22865 at 4770.099 kb on + strand, within BWI76_RS22865 at 4770.399 kb on + strand, within BWI76_RS22865 at 4770.415 kb on - strand, within BWI76_RS22865 at 4770.442 kb on + strand, within BWI76_RS22865 at 4770.830 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 4,768,370 + -0.4 4,768,428 + BWI76_RS22855 0.27 -1.9 4,768,530 + BWI76_RS22855 0.58 -0.5 4,768,538 - BWI76_RS22855 0.60 -0.2 4,768,610 - BWI76_RS22855 0.82 +0.5 4,768,748 - +6.9 4,768,748 - -0.9 4,768,803 - +0.6 4,768,859 - +1.3 4,768,902 - -3.8 4,768,904 + +0.4 4,768,985 - +0.3 4,769,116 + -0.2 4,769,378 - BWI76_RS22860 0.14 +1.2 4,769,683 - BWI76_RS22860 0.70 +1.4 4,769,798 + +0.2 4,769,826 + -1.2 4,769,834 - -1.4 4,769,880 - +0.2 4,769,948 - -2.8 4,770,001 - -0.5 4,770,003 + BWI76_RS22865 0.10 -0.8 4,770,011 - BWI76_RS22865 0.11 +1.1 4,770,061 - BWI76_RS22865 0.17 -0.6 4,770,091 + BWI76_RS22865 0.21 -0.4 4,770,099 + BWI76_RS22865 0.22 +0.3 4,770,399 + BWI76_RS22865 0.57 -0.1 4,770,415 - BWI76_RS22865 0.59 -2.6 4,770,442 + BWI76_RS22865 0.62 +1.8 4,770,830 + -3.3
Or see this region's nucleotide sequence