Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21100

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21095 and BWI76_RS21100 are separated by 288 nucleotidesBWI76_RS21100 and BWI76_RS21105 are separated by 162 nucleotides BWI76_RS21095: BWI76_RS21095 - bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN, at 4,413,632 to 4,414,798 _RS21095 BWI76_RS21100: BWI76_RS21100 - nucleoside-diphosphate kinase, at 4,415,087 to 4,415,518 _RS21100 BWI76_RS21105: BWI76_RS21105 - penicillin-binding protein 1C, at 4,415,681 to 4,418,005 _RS21105 Position (kb) 4415 4416Strain fitness (log2 ratio) -2 -1 0 1 2at 4414.089 kb on + strand, within BWI76_RS21095at 4414.374 kb on - strand, within BWI76_RS21095at 4414.438 kb on + strand, within BWI76_RS21095at 4414.468 kb on - strand, within BWI76_RS21095at 4414.471 kb on - strand, within BWI76_RS21095at 4414.505 kb on + strand, within BWI76_RS21095at 4414.513 kb on - strand, within BWI76_RS21095at 4414.639 kb on - strand, within BWI76_RS21095at 4414.713 kb on - strandat 4414.713 kb on - strandat 4414.765 kb on - strandat 4414.961 kb on - strandat 4414.963 kb on - strandat 4415.308 kb on + strand, within BWI76_RS21100at 4415.391 kb on - strand, within BWI76_RS21100at 4415.556 kb on - strandat 4415.570 kb on - strandat 4415.775 kb on - strandat 4415.775 kb on - strandat 4415.880 kb on + strandat 4416.032 kb on - strand, within BWI76_RS21105at 4416.244 kb on - strand, within BWI76_RS21105at 4416.293 kb on + strand, within BWI76_RS21105

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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4,414,089 + BWI76_RS21095 0.39 -2.2
4,414,374 - BWI76_RS21095 0.64 +0.2
4,414,438 + BWI76_RS21095 0.69 +0.8
4,414,468 - BWI76_RS21095 0.72 -0.9
4,414,471 - BWI76_RS21095 0.72 -0.5
4,414,505 + BWI76_RS21095 0.75 -0.4
4,414,513 - BWI76_RS21095 0.75 +1.9
4,414,639 - BWI76_RS21095 0.86 +0.2
4,414,713 - -0.6
4,414,713 - +0.5
4,414,765 - -0.0
4,414,961 - +0.2
4,414,963 - -0.4
4,415,308 + BWI76_RS21100 0.51 -2.3
4,415,391 - BWI76_RS21100 0.70 -2.0
4,415,556 - +0.7
4,415,570 - -0.5
4,415,775 - +0.2
4,415,775 - +1.2
4,415,880 + -2.0
4,416,032 - BWI76_RS21105 0.15 -0.4
4,416,244 - BWI76_RS21105 0.24 -1.3
4,416,293 + BWI76_RS21105 0.26 -0.2

Or see this region's nucleotide sequence