Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20130

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20125 and BWI76_RS20130 are separated by 202 nucleotidesBWI76_RS20130 and BWI76_RS20135 are separated by 6 nucleotides BWI76_RS20125: BWI76_RS20125 - integrase, at 4,216,233 to 4,216,784 _RS20125 BWI76_RS20130: BWI76_RS20130 - NADH-quinone oxidoreductase subunit N, at 4,216,987 to 4,218,444 _RS20130 BWI76_RS20135: BWI76_RS20135 - NADH-quinone oxidoreductase subunit M, at 4,218,451 to 4,219,980 _RS20135 Position (kb) 4216 4217 4218 4219Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4216.028 kb on - strandat 4216.073 kb on - strandat 4216.074 kb on - strandat 4216.336 kb on + strand, within BWI76_RS20125at 4216.357 kb on - strand, within BWI76_RS20125at 4216.449 kb on + strand, within BWI76_RS20125at 4216.529 kb on + strand, within BWI76_RS20125at 4216.538 kb on + strand, within BWI76_RS20125at 4216.539 kb on - strand, within BWI76_RS20125at 4216.545 kb on - strand, within BWI76_RS20125at 4216.546 kb on - strand, within BWI76_RS20125at 4216.666 kb on - strand, within BWI76_RS20125at 4216.719 kb on + strand, within BWI76_RS20125at 4216.719 kb on + strand, within BWI76_RS20125at 4216.723 kb on - strand, within BWI76_RS20125at 4216.727 kb on - strand, within BWI76_RS20125at 4216.728 kb on - strand, within BWI76_RS20125at 4216.740 kb on + strandat 4216.741 kb on + strandat 4216.798 kb on + strandat 4216.798 kb on + strandat 4216.806 kb on - strandat 4216.808 kb on + strandat 4216.811 kb on - strandat 4216.958 kb on - strandat 4216.983 kb on - strandat 4216.990 kb on + strandat 4216.993 kb on - strandat 4216.993 kb on - strandat 4217.006 kb on - strandat 4217.010 kb on + strandat 4217.010 kb on + strandat 4217.015 kb on + strandat 4217.015 kb on + strandat 4217.020 kb on + strandat 4217.028 kb on - strandat 4217.042 kb on + strandat 4217.046 kb on + strandat 4217.050 kb on - strandat 4217.050 kb on - strandat 4217.090 kb on + strandat 4217.145 kb on + strand, within BWI76_RS20130at 4217.145 kb on + strand, within BWI76_RS20130at 4217.160 kb on - strand, within BWI76_RS20130at 4217.427 kb on + strand, within BWI76_RS20130at 4217.435 kb on - strand, within BWI76_RS20130at 4217.909 kb on + strand, within BWI76_RS20130at 4218.271 kb on + strand, within BWI76_RS20130at 4218.731 kb on + strand, within BWI76_RS20135at 4219.060 kb on + strand, within BWI76_RS20135

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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4,216,028 - -0.9
4,216,073 - -0.7
4,216,074 - +2.5
4,216,336 + BWI76_RS20125 0.19 -1.7
4,216,357 - BWI76_RS20125 0.22 -0.0
4,216,449 + BWI76_RS20125 0.39 -0.2
4,216,529 + BWI76_RS20125 0.54 -0.1
4,216,538 + BWI76_RS20125 0.55 -0.2
4,216,539 - BWI76_RS20125 0.55 +1.4
4,216,545 - BWI76_RS20125 0.57 -2.8
4,216,546 - BWI76_RS20125 0.57 -0.9
4,216,666 - BWI76_RS20125 0.78 -1.5
4,216,719 + BWI76_RS20125 0.88 +0.0
4,216,719 + BWI76_RS20125 0.88 +1.2
4,216,723 - BWI76_RS20125 0.89 -0.3
4,216,727 - BWI76_RS20125 0.89 -1.2
4,216,728 - BWI76_RS20125 0.90 -1.3
4,216,740 + +0.3
4,216,741 + +0.1
4,216,798 + +2.3
4,216,798 + +0.1
4,216,806 - -0.8
4,216,808 + +0.4
4,216,811 - -0.3
4,216,958 - +1.5
4,216,983 - -0.7
4,216,990 + -0.9
4,216,993 - -2.0
4,216,993 - -0.9
4,217,006 - -1.4
4,217,010 + -1.1
4,217,010 + +0.5
4,217,015 + -1.2
4,217,015 + -1.2
4,217,020 + -0.8
4,217,028 - +2.4
4,217,042 + +2.3
4,217,046 + -1.4
4,217,050 - -2.9
4,217,050 - -2.5
4,217,090 + -1.6
4,217,145 + BWI76_RS20130 0.11 -1.7
4,217,145 + BWI76_RS20130 0.11 -0.9
4,217,160 - BWI76_RS20130 0.12 -1.6
4,217,427 + BWI76_RS20130 0.30 -3.6
4,217,435 - BWI76_RS20130 0.31 -2.7
4,217,909 + BWI76_RS20130 0.63 -3.3
4,218,271 + BWI76_RS20130 0.88 -0.5
4,218,731 + BWI76_RS20135 0.18 -2.7
4,219,060 + BWI76_RS20135 0.40 -1.2

Or see this region's nucleotide sequence