Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12180 and BWI76_RS12185 overlap by 11 nucleotides BWI76_RS12185 and BWI76_RS12190 are separated by 7 nucleotides BWI76_RS12190 and BWI76_RS12195 are separated by 2 nucleotides BWI76_RS12195 and BWI76_RS12200 overlap by 50 nucleotides
BWI76_RS12180: BWI76_RS12180 - membrane protein, at 2,552,135 to 2,552,524
_RS12180
BWI76_RS12185: BWI76_RS12185 - hypothetical protein, at 2,552,514 to 2,552,792
_RS12185
BWI76_RS12190: BWI76_RS12190 - glycoside hydrolase family protein, at 2,552,800 to 2,553,429
_RS12190
BWI76_RS12195: BWI76_RS12195 - hypothetical protein, at 2,553,432 to 2,553,707
_RS12195
BWI76_RS12200: BWI76_RS12200 - hypothetical protein, at 2,553,658 to 2,553,852
_RS12200
Position (kb)
2552
2553 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2551.516 kb on - strand at 2551.520 kb on + strand at 2551.530 kb on + strand at 2551.548 kb on + strand at 2551.741 kb on + strand at 2551.791 kb on - strand at 2551.806 kb on + strand at 2551.855 kb on + strand at 2551.855 kb on + strand at 2551.855 kb on + strand at 2551.884 kb on - strand at 2551.930 kb on - strand at 2551.951 kb on + strand at 2552.025 kb on - strand at 2552.116 kb on + strand at 2552.118 kb on + strand at 2552.119 kb on - strand at 2552.124 kb on - strand at 2552.179 kb on + strand, within BWI76_RS12180 at 2552.179 kb on + strand, within BWI76_RS12180 at 2552.187 kb on - strand, within BWI76_RS12180 at 2552.337 kb on + strand, within BWI76_RS12180 at 2552.337 kb on + strand, within BWI76_RS12180 at 2552.342 kb on + strand, within BWI76_RS12180 at 2552.342 kb on + strand, within BWI76_RS12180 at 2552.342 kb on + strand, within BWI76_RS12180 at 2552.360 kb on - strand, within BWI76_RS12180 at 2552.370 kb on + strand, within BWI76_RS12180 at 2552.375 kb on + strand, within BWI76_RS12180 at 2552.502 kb on - strand at 2552.596 kb on + strand, within BWI76_RS12185 at 2552.606 kb on - strand, within BWI76_RS12185 at 2552.653 kb on - strand, within BWI76_RS12185 at 2553.015 kb on + strand at 2553.033 kb on + strand, within BWI76_RS12190 at 2553.033 kb on + strand, within BWI76_RS12190 at 2553.036 kb on - strand, within BWI76_RS12190 at 2553.036 kb on - strand, within BWI76_RS12190 at 2553.045 kb on + strand at 2553.047 kb on + strand, within BWI76_RS12190 at 2553.054 kb on + strand at 2553.081 kb on - strand, within BWI76_RS12190 at 2553.140 kb on - strand, within BWI76_RS12190 at 2553.140 kb on - strand, within BWI76_RS12190 at 2553.160 kb on - strand, within BWI76_RS12190 at 2553.160 kb on - strand, within BWI76_RS12190 at 2553.287 kb on - strand, within BWI76_RS12190 at 2553.287 kb on - strand, within BWI76_RS12190 at 2553.289 kb on + strand, within BWI76_RS12190 at 2553.400 kb on - strand at 2553.496 kb on + strand, within BWI76_RS12195 at 2553.504 kb on - strand, within BWI76_RS12195 at 2553.553 kb on - strand, within BWI76_RS12195 at 2553.578 kb on - strand, within BWI76_RS12195 at 2553.615 kb on - strand at 2553.627 kb on - strand, within BWI76_RS12195 at 2553.719 kb on + strand, within BWI76_RS12200 at 2553.751 kb on - strand, within BWI76_RS12200
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 2,551,516 - -1.8 2,551,520 + -0.4 2,551,530 + -0.4 2,551,548 + -0.6 2,551,741 + -0.1 2,551,791 - +0.9 2,551,806 + -1.4 2,551,855 + -0.1 2,551,855 + -1.1 2,551,855 + -1.5 2,551,884 - -4.3 2,551,930 - +0.2 2,551,951 + +0.4 2,552,025 - -1.3 2,552,116 + -0.4 2,552,118 + -0.3 2,552,119 - +1.3 2,552,124 - +0.9 2,552,179 + BWI76_RS12180 0.11 -0.4 2,552,179 + BWI76_RS12180 0.11 +1.1 2,552,187 - BWI76_RS12180 0.13 -0.6 2,552,337 + BWI76_RS12180 0.52 -1.6 2,552,337 + BWI76_RS12180 0.52 -0.1 2,552,342 + BWI76_RS12180 0.53 -1.8 2,552,342 + BWI76_RS12180 0.53 -1.4 2,552,342 + BWI76_RS12180 0.53 +0.1 2,552,360 - BWI76_RS12180 0.58 -2.0 2,552,370 + BWI76_RS12180 0.60 -0.4 2,552,375 + BWI76_RS12180 0.62 -0.1 2,552,502 - +0.9 2,552,596 + BWI76_RS12185 0.29 -2.2 2,552,606 - BWI76_RS12185 0.33 +0.7 2,552,653 - BWI76_RS12185 0.50 -0.4 2,553,015 + +1.4 2,553,033 + BWI76_RS12190 0.37 +0.2 2,553,033 + BWI76_RS12190 0.37 -1.2 2,553,036 - BWI76_RS12190 0.37 -0.7 2,553,036 - BWI76_RS12190 0.37 -0.1 2,553,045 + -0.6 2,553,047 + BWI76_RS12190 0.39 -3.1 2,553,054 + +0.2 2,553,081 - BWI76_RS12190 0.45 -0.4 2,553,140 - BWI76_RS12190 0.54 -0.9 2,553,140 - BWI76_RS12190 0.54 +0.0 2,553,160 - BWI76_RS12190 0.57 -0.7 2,553,160 - BWI76_RS12190 0.57 -1.5 2,553,287 - BWI76_RS12190 0.77 +0.2 2,553,287 - BWI76_RS12190 0.77 -4.6 2,553,289 + BWI76_RS12190 0.78 +0.8 2,553,400 - +0.1 2,553,496 + BWI76_RS12195 0.23 +0.9 2,553,504 - BWI76_RS12195 0.26 -0.8 2,553,553 - BWI76_RS12195 0.44 -2.2 2,553,578 - BWI76_RS12195 0.53 +0.4 2,553,615 - +1.7 2,553,627 - BWI76_RS12195 0.71 -0.6 2,553,719 + BWI76_RS12200 0.31 -0.1 2,553,751 - BWI76_RS12200 0.48 -1.0
Or see this region's nucleotide sequence