Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12185

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12180 and BWI76_RS12185 overlap by 11 nucleotidesBWI76_RS12185 and BWI76_RS12190 are separated by 7 nucleotidesBWI76_RS12190 and BWI76_RS12195 are separated by 2 nucleotidesBWI76_RS12195 and BWI76_RS12200 overlap by 50 nucleotides BWI76_RS12180: BWI76_RS12180 - membrane protein, at 2,552,135 to 2,552,524 _RS12180 BWI76_RS12185: BWI76_RS12185 - hypothetical protein, at 2,552,514 to 2,552,792 _RS12185 BWI76_RS12190: BWI76_RS12190 - glycoside hydrolase family protein, at 2,552,800 to 2,553,429 _RS12190 BWI76_RS12195: BWI76_RS12195 - hypothetical protein, at 2,553,432 to 2,553,707 _RS12195 BWI76_RS12200: BWI76_RS12200 - hypothetical protein, at 2,553,658 to 2,553,852 _RS12200 Position (kb) 2552 2553Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2551.516 kb on - strandat 2551.520 kb on + strandat 2551.530 kb on + strandat 2551.548 kb on + strandat 2551.741 kb on + strandat 2551.791 kb on - strandat 2551.806 kb on + strandat 2551.855 kb on + strandat 2551.855 kb on + strandat 2551.855 kb on + strandat 2551.884 kb on - strandat 2551.930 kb on - strandat 2551.951 kb on + strandat 2552.025 kb on - strandat 2552.116 kb on + strandat 2552.118 kb on + strandat 2552.119 kb on - strandat 2552.124 kb on - strandat 2552.179 kb on + strand, within BWI76_RS12180at 2552.179 kb on + strand, within BWI76_RS12180at 2552.187 kb on - strand, within BWI76_RS12180at 2552.337 kb on + strand, within BWI76_RS12180at 2552.337 kb on + strand, within BWI76_RS12180at 2552.342 kb on + strand, within BWI76_RS12180at 2552.342 kb on + strand, within BWI76_RS12180at 2552.342 kb on + strand, within BWI76_RS12180at 2552.360 kb on - strand, within BWI76_RS12180at 2552.370 kb on + strand, within BWI76_RS12180at 2552.375 kb on + strand, within BWI76_RS12180at 2552.502 kb on - strandat 2552.596 kb on + strand, within BWI76_RS12185at 2552.606 kb on - strand, within BWI76_RS12185at 2552.653 kb on - strand, within BWI76_RS12185at 2553.015 kb on + strandat 2553.033 kb on + strand, within BWI76_RS12190at 2553.033 kb on + strand, within BWI76_RS12190at 2553.036 kb on - strand, within BWI76_RS12190at 2553.036 kb on - strand, within BWI76_RS12190at 2553.045 kb on + strandat 2553.047 kb on + strand, within BWI76_RS12190at 2553.054 kb on + strandat 2553.081 kb on - strand, within BWI76_RS12190at 2553.140 kb on - strand, within BWI76_RS12190at 2553.140 kb on - strand, within BWI76_RS12190at 2553.160 kb on - strand, within BWI76_RS12190at 2553.160 kb on - strand, within BWI76_RS12190at 2553.287 kb on - strand, within BWI76_RS12190at 2553.287 kb on - strand, within BWI76_RS12190at 2553.289 kb on + strand, within BWI76_RS12190at 2553.400 kb on - strandat 2553.496 kb on + strand, within BWI76_RS12195at 2553.504 kb on - strand, within BWI76_RS12195at 2553.553 kb on - strand, within BWI76_RS12195at 2553.578 kb on - strand, within BWI76_RS12195at 2553.615 kb on - strandat 2553.627 kb on - strand, within BWI76_RS12195at 2553.719 kb on + strand, within BWI76_RS12200at 2553.751 kb on - strand, within BWI76_RS12200

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,551,516 - -1.8
2,551,520 + -0.4
2,551,530 + -0.4
2,551,548 + -0.6
2,551,741 + -0.1
2,551,791 - +0.9
2,551,806 + -1.4
2,551,855 + -0.1
2,551,855 + -1.1
2,551,855 + -1.5
2,551,884 - -4.3
2,551,930 - +0.2
2,551,951 + +0.4
2,552,025 - -1.3
2,552,116 + -0.4
2,552,118 + -0.3
2,552,119 - +1.3
2,552,124 - +0.9
2,552,179 + BWI76_RS12180 0.11 -0.4
2,552,179 + BWI76_RS12180 0.11 +1.1
2,552,187 - BWI76_RS12180 0.13 -0.6
2,552,337 + BWI76_RS12180 0.52 -1.6
2,552,337 + BWI76_RS12180 0.52 -0.1
2,552,342 + BWI76_RS12180 0.53 -1.8
2,552,342 + BWI76_RS12180 0.53 -1.4
2,552,342 + BWI76_RS12180 0.53 +0.1
2,552,360 - BWI76_RS12180 0.58 -2.0
2,552,370 + BWI76_RS12180 0.60 -0.4
2,552,375 + BWI76_RS12180 0.62 -0.1
2,552,502 - +0.9
2,552,596 + BWI76_RS12185 0.29 -2.2
2,552,606 - BWI76_RS12185 0.33 +0.7
2,552,653 - BWI76_RS12185 0.50 -0.4
2,553,015 + +1.4
2,553,033 + BWI76_RS12190 0.37 +0.2
2,553,033 + BWI76_RS12190 0.37 -1.2
2,553,036 - BWI76_RS12190 0.37 -0.7
2,553,036 - BWI76_RS12190 0.37 -0.1
2,553,045 + -0.6
2,553,047 + BWI76_RS12190 0.39 -3.1
2,553,054 + +0.2
2,553,081 - BWI76_RS12190 0.45 -0.4
2,553,140 - BWI76_RS12190 0.54 -0.9
2,553,140 - BWI76_RS12190 0.54 +0.0
2,553,160 - BWI76_RS12190 0.57 -0.7
2,553,160 - BWI76_RS12190 0.57 -1.5
2,553,287 - BWI76_RS12190 0.77 +0.2
2,553,287 - BWI76_RS12190 0.77 -4.6
2,553,289 + BWI76_RS12190 0.78 +0.8
2,553,400 - +0.1
2,553,496 + BWI76_RS12195 0.23 +0.9
2,553,504 - BWI76_RS12195 0.26 -0.8
2,553,553 - BWI76_RS12195 0.44 -2.2
2,553,578 - BWI76_RS12195 0.53 +0.4
2,553,615 - +1.7
2,553,627 - BWI76_RS12195 0.71 -0.6
2,553,719 + BWI76_RS12200 0.31 -0.1
2,553,751 - BWI76_RS12200 0.48 -1.0

Or see this region's nucleotide sequence