Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11780

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11775 and BWI76_RS11780 are separated by 148 nucleotidesBWI76_RS11780 and BWI76_RS11785 are separated by 130 nucleotidesBWI76_RS11785 and BWI76_RS11790 are separated by 32 nucleotides BWI76_RS11775: BWI76_RS11775 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,477,535 to 2,478,296 _RS11775 BWI76_RS11780: BWI76_RS11780 - 2-deoxyglucose-6-phosphatase, at 2,478,445 to 2,479,113 _RS11780 BWI76_RS11785: BWI76_RS11785 - hypothetical protein, at 2,479,244 to 2,479,780 _RS11785 BWI76_RS11790: BWI76_RS11790 - acyl-CoA thioesterase, at 2,479,813 to 2,480,211 _RS11790 Position (kb) 2478 2479 2480Strain fitness (log2 ratio) -1 0 1at 2477.451 kb on + strandat 2477.575 kb on - strandat 2477.628 kb on - strand, within BWI76_RS11775at 2477.829 kb on - strand, within BWI76_RS11775at 2478.275 kb on + strandat 2478.283 kb on - strandat 2478.340 kb on + strandat 2478.613 kb on + strand, within BWI76_RS11780at 2478.926 kb on + strand, within BWI76_RS11780at 2479.249 kb on - strandat 2479.249 kb on - strandat 2479.401 kb on + strand, within BWI76_RS11785

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,477,451 + -1.1
2,477,575 - +1.0
2,477,628 - BWI76_RS11775 0.12 -1.1
2,477,829 - BWI76_RS11775 0.39 -0.6
2,478,275 + +0.6
2,478,283 - -0.1
2,478,340 + +0.0
2,478,613 + BWI76_RS11780 0.25 -1.2
2,478,926 + BWI76_RS11780 0.72 -1.5
2,479,249 - -0.4
2,479,249 - +1.6
2,479,401 + BWI76_RS11785 0.29 -1.1

Or see this region's nucleotide sequence