Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10285

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10275 and BWI76_RS10280 overlap by 4 nucleotidesBWI76_RS10280 and BWI76_RS10285 are separated by 28 nucleotidesBWI76_RS10285 and BWI76_RS10290 are separated by 191 nucleotides BWI76_RS10275: BWI76_RS10275 - ABC transporter, at 2,176,334 to 2,177,074 _RS10275 BWI76_RS10280: BWI76_RS10280 - polar amino acid ABC transporter permease, at 2,177,071 to 2,177,796 _RS10280 BWI76_RS10285: BWI76_RS10285 - family 3 extracellular solute-binding protein, at 2,177,825 to 2,178,637 _RS10285 BWI76_RS10290: BWI76_RS10290 - transcriptional regulator, at 2,178,829 to 2,179,725 _RS10290 Position (kb) 2177 2178 2179Strain fitness (log2 ratio) -1 0 1 2at 2176.990 kb on + strand, within BWI76_RS10275at 2177.183 kb on + strand, within BWI76_RS10280at 2177.191 kb on - strand, within BWI76_RS10280at 2177.663 kb on + strand, within BWI76_RS10280at 2177.696 kb on + strand, within BWI76_RS10280at 2177.701 kb on + strand, within BWI76_RS10280at 2177.706 kb on + strand, within BWI76_RS10280at 2177.793 kb on + strandat 2177.981 kb on - strand, within BWI76_RS10285at 2178.350 kb on - strand, within BWI76_RS10285at 2178.471 kb on + strand, within BWI76_RS10285at 2178.488 kb on - strand, within BWI76_RS10285at 2178.842 kb on - strandat 2179.198 kb on + strand, within BWI76_RS10290at 2179.198 kb on + strand, within BWI76_RS10290at 2179.206 kb on - strand, within BWI76_RS10290at 2179.206 kb on - strand, within BWI76_RS10290at 2179.255 kb on + strand, within BWI76_RS10290at 2179.499 kb on + strand, within BWI76_RS10290at 2179.507 kb on - strandat 2179.520 kb on + strandat 2179.622 kb on + strand, within BWI76_RS10290

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,176,990 + BWI76_RS10275 0.89 +0.6
2,177,183 + BWI76_RS10280 0.15 -1.4
2,177,191 - BWI76_RS10280 0.17 -0.3
2,177,663 + BWI76_RS10280 0.82 +0.1
2,177,696 + BWI76_RS10280 0.86 -0.9
2,177,701 + BWI76_RS10280 0.87 -0.3
2,177,706 + BWI76_RS10280 0.87 -1.0
2,177,793 + +0.1
2,177,981 - BWI76_RS10285 0.19 -0.9
2,178,350 - BWI76_RS10285 0.65 -0.0
2,178,471 + BWI76_RS10285 0.79 -0.8
2,178,488 - BWI76_RS10285 0.82 -0.5
2,178,842 - -0.2
2,179,198 + BWI76_RS10290 0.41 +0.2
2,179,198 + BWI76_RS10290 0.41 -0.3
2,179,206 - BWI76_RS10290 0.42 +1.6
2,179,206 - BWI76_RS10290 0.42 +1.3
2,179,255 + BWI76_RS10290 0.47 +2.1
2,179,499 + BWI76_RS10290 0.75 -0.4
2,179,507 - -1.4
2,179,520 + +1.2
2,179,622 + BWI76_RS10290 0.88 +1.2

Or see this region's nucleotide sequence