Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10270

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10265 and BWI76_RS10270 overlap by 4 nucleotidesBWI76_RS10270 and BWI76_RS10275 are separated by 13 nucleotidesBWI76_RS10275 and BWI76_RS10280 overlap by 4 nucleotides BWI76_RS10265: BWI76_RS10265 - mandelate racemase/muconate lactonizing protein, at 2,174,048 to 2,175,148 _RS10265 BWI76_RS10270: BWI76_RS10270 - acetylornithine deacetylase, at 2,175,145 to 2,176,320 _RS10270 BWI76_RS10275: BWI76_RS10275 - ABC transporter, at 2,176,334 to 2,177,074 _RS10275 BWI76_RS10280: BWI76_RS10280 - polar amino acid ABC transporter permease, at 2,177,071 to 2,177,796 _RS10280 Position (kb) 2175 2176 2177Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2174.399 kb on + strand, within BWI76_RS10265at 2174.485 kb on - strand, within BWI76_RS10265at 2174.485 kb on - strand, within BWI76_RS10265at 2174.799 kb on - strand, within BWI76_RS10265at 2174.854 kb on - strand, within BWI76_RS10265at 2174.904 kb on - strand, within BWI76_RS10265at 2175.283 kb on - strand, within BWI76_RS10270at 2175.320 kb on - strand, within BWI76_RS10270at 2175.825 kb on - strand, within BWI76_RS10270at 2176.089 kb on - strand, within BWI76_RS10270at 2176.116 kb on - strand, within BWI76_RS10270at 2176.353 kb on + strandat 2176.494 kb on - strand, within BWI76_RS10275at 2176.517 kb on - strand, within BWI76_RS10275at 2176.813 kb on + strand, within BWI76_RS10275at 2176.990 kb on + strand, within BWI76_RS10275at 2177.183 kb on + strand, within BWI76_RS10280at 2177.191 kb on - strand, within BWI76_RS10280

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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2,174,399 + BWI76_RS10265 0.32 -0.8
2,174,485 - BWI76_RS10265 0.40 -1.0
2,174,485 - BWI76_RS10265 0.40 -0.4
2,174,799 - BWI76_RS10265 0.68 +0.5
2,174,854 - BWI76_RS10265 0.73 -2.3
2,174,904 - BWI76_RS10265 0.78 -0.7
2,175,283 - BWI76_RS10270 0.12 +0.8
2,175,320 - BWI76_RS10270 0.15 -2.9
2,175,825 - BWI76_RS10270 0.58 +0.4
2,176,089 - BWI76_RS10270 0.80 -0.3
2,176,116 - BWI76_RS10270 0.83 +0.4
2,176,353 + +4.4
2,176,494 - BWI76_RS10275 0.22 -2.1
2,176,517 - BWI76_RS10275 0.25 -0.5
2,176,813 + BWI76_RS10275 0.65 +0.9
2,176,990 + BWI76_RS10275 0.89 +0.6
2,177,183 + BWI76_RS10280 0.15 -1.4
2,177,191 - BWI76_RS10280 0.17 -0.3

Or see this region's nucleotide sequence