Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08530

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08520 and BWI76_RS08525 are separated by 3 nucleotidesBWI76_RS08525 and BWI76_RS08530 are separated by 105 nucleotidesBWI76_RS08530 and BWI76_RS08535 are separated by 128 nucleotides BWI76_RS08520: BWI76_RS08520 - colicin transporter TolQ, at 1,803,797 to 1,804,480 _RS08520 BWI76_RS08525: BWI76_RS08525 - colicin transporter, at 1,804,484 to 1,804,912 _RS08525 BWI76_RS08530: BWI76_RS08530 - protein TolA, at 1,805,018 to 1,806,355 _RS08530 BWI76_RS08535: BWI76_RS08535 - translocation protein TolB, at 1,806,484 to 1,807,776 _RS08535 Position (kb) 1805 1806 1807Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1804.358 kb on + strand, within BWI76_RS08520at 1804.368 kb on + strand, within BWI76_RS08520at 1804.421 kb on - strandat 1804.433 kb on + strandat 1804.486 kb on + strandat 1804.491 kb on + strandat 1804.546 kb on + strand, within BWI76_RS08525at 1804.697 kb on + strand, within BWI76_RS08525at 1804.753 kb on + strand, within BWI76_RS08525at 1805.247 kb on + strand, within BWI76_RS08530at 1806.168 kb on - strand, within BWI76_RS08530at 1806.468 kb on + strandat 1806.476 kb on - strandat 1806.619 kb on + strand, within BWI76_RS08535at 1806.763 kb on - strandat 1807.007 kb on - strand, within BWI76_RS08535at 1807.291 kb on - strand, within BWI76_RS08535

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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1,804,358 + BWI76_RS08520 0.82 -2.7
1,804,368 + BWI76_RS08520 0.83 -2.0
1,804,421 - -0.8
1,804,433 + -3.6
1,804,486 + -2.7
1,804,491 + -1.6
1,804,546 + BWI76_RS08525 0.14 -1.9
1,804,697 + BWI76_RS08525 0.50 +0.3
1,804,753 + BWI76_RS08525 0.63 +2.9
1,805,247 + BWI76_RS08530 0.17 -2.5
1,806,168 - BWI76_RS08530 0.86 -2.8
1,806,468 + -1.4
1,806,476 - +1.9
1,806,619 + BWI76_RS08535 0.10 -1.0
1,806,763 - +0.0
1,807,007 - BWI76_RS08535 0.40 -0.8
1,807,291 - BWI76_RS08535 0.62 +1.5

Or see this region's nucleotide sequence