Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08255

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08250 and BWI76_RS08255 overlap by 14 nucleotidesBWI76_RS08255 and BWI76_RS08260 are separated by 97 nucleotides BWI76_RS08250: BWI76_RS08250 - tricarballylate utilization protein B, at 1,746,452 to 1,747,591 _RS08250 BWI76_RS08255: BWI76_RS08255 - tricarballylate dehydrogenase, at 1,747,578 to 1,748,981 _RS08255 BWI76_RS08260: BWI76_RS08260 - LysR family transcriptional regulator, at 1,749,079 to 1,750,005 _RS08260 Position (kb) 1747 1748 1749Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7at 1746.739 kb on + strand, within BWI76_RS08250at 1746.936 kb on - strand, within BWI76_RS08250at 1746.936 kb on - strand, within BWI76_RS08250at 1747.282 kb on - strand, within BWI76_RS08250at 1747.506 kb on + strandat 1747.605 kb on - strandat 1748.208 kb on + strand, within BWI76_RS08255at 1748.216 kb on - strand, within BWI76_RS08255at 1748.299 kb on + strand, within BWI76_RS08255at 1748.336 kb on + strand, within BWI76_RS08255at 1748.344 kb on - strand, within BWI76_RS08255at 1748.584 kb on + strand, within BWI76_RS08255at 1748.660 kb on + strand, within BWI76_RS08255at 1748.666 kb on + strand, within BWI76_RS08255at 1749.594 kb on + strand, within BWI76_RS08260at 1749.845 kb on - strand, within BWI76_RS08260

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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1,746,739 + BWI76_RS08250 0.25 -0.2
1,746,936 - BWI76_RS08250 0.42 -0.3
1,746,936 - BWI76_RS08250 0.42 -0.0
1,747,282 - BWI76_RS08250 0.73 +0.5
1,747,506 + +0.0
1,747,605 - +0.3
1,748,208 + BWI76_RS08255 0.45 -0.0
1,748,216 - BWI76_RS08255 0.45 +0.4
1,748,299 + BWI76_RS08255 0.51 -2.3
1,748,336 + BWI76_RS08255 0.54 +0.5
1,748,344 - BWI76_RS08255 0.55 +6.9
1,748,584 + BWI76_RS08255 0.72 +0.2
1,748,660 + BWI76_RS08255 0.77 -2.9
1,748,666 + BWI76_RS08255 0.77 -0.6
1,749,594 + BWI76_RS08260 0.56 -0.4
1,749,845 - BWI76_RS08260 0.83 +0.4

Or see this region's nucleotide sequence