Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06340

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06335 and BWI76_RS06340 are separated by 295 nucleotidesBWI76_RS06340 and BWI76_RS06345 are separated by 147 nucleotidesBWI76_RS06345 and BWI76_RS06350 are separated by 4 nucleotides BWI76_RS06335: BWI76_RS06335 - nucleoside-specific channel-forming protein Tsx, at 1,333,308 to 1,334,192 _RS06335 BWI76_RS06340: BWI76_RS06340 - hypothetical protein, at 1,334,488 to 1,335,030 _RS06340 BWI76_RS06345: BWI76_RS06345 - transcriptional regulator NrdR, at 1,335,178 to 1,335,627 _RS06345 BWI76_RS06350: BWI76_RS06350 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase, at 1,335,632 to 1,336,735 _RS06350 Position (kb) 1334 1335 1336Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1333.544 kb on + strand, within BWI76_RS06335at 1333.544 kb on + strand, within BWI76_RS06335at 1333.751 kb on - strand, within BWI76_RS06335at 1334.154 kb on + strandat 1334.154 kb on + strandat 1334.154 kb on + strandat 1334.154 kb on + strandat 1334.154 kb on + strandat 1334.205 kb on + strandat 1334.213 kb on - strandat 1334.217 kb on + strandat 1334.267 kb on - strandat 1334.457 kb on - strandat 1334.573 kb on - strand, within BWI76_RS06340at 1335.621 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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1,333,544 + BWI76_RS06335 0.27 -1.7
1,333,544 + BWI76_RS06335 0.27 -1.5
1,333,751 - BWI76_RS06335 0.50 -0.5
1,334,154 + +0.9
1,334,154 + -0.5
1,334,154 + -0.4
1,334,154 + +0.4
1,334,154 + -0.0
1,334,205 + -1.0
1,334,213 - -0.2
1,334,217 + -0.7
1,334,267 - -0.0
1,334,457 - +0.3
1,334,573 - BWI76_RS06340 0.16 -3.6
1,335,621 + +2.4

Or see this region's nucleotide sequence