Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03300

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03290 and BWI76_RS03295 are separated by 72 nucleotidesBWI76_RS03295 and BWI76_RS03300 are separated by 32 nucleotidesBWI76_RS03300 and BWI76_RS03305 overlap by 32 nucleotides BWI76_RS03290: BWI76_RS03290 - hypothetical protein, at 679,037 to 679,962 _RS03290 BWI76_RS03295: BWI76_RS03295 - transposase, at 680,035 to 680,298 _RS03295 BWI76_RS03300: BWI76_RS03300 - hypothetical protein, at 680,331 to 681,125 _RS03300 BWI76_RS03305: BWI76_RS03305 - hypothetical protein, at 681,094 to 682,334 _RS03305 Position (kb) 680 681 682Strain fitness (log2 ratio) -2 -1 0 1at 679.369 kb on + strand, within BWI76_RS03290at 679.507 kb on + strand, within BWI76_RS03290at 679.546 kb on + strand, within BWI76_RS03290at 679.657 kb on + strand, within BWI76_RS03290at 679.657 kb on + strand, within BWI76_RS03290at 679.880 kb on + strandat 679.980 kb on + strandat 679.989 kb on + strandat 679.998 kb on - strandat 680.006 kb on + strandat 680.015 kb on + strandat 680.020 kb on + strandat 680.142 kb on + strand, within BWI76_RS03295at 680.188 kb on - strand, within BWI76_RS03295at 680.256 kb on + strandat 680.275 kb on - strandat 680.327 kb on + strandat 680.336 kb on - strandat 680.494 kb on + strand, within BWI76_RS03300at 680.529 kb on + strand, within BWI76_RS03300at 680.695 kb on + strand, within BWI76_RS03300at 680.836 kb on - strand, within BWI76_RS03300at 680.893 kb on + strand, within BWI76_RS03300at 680.895 kb on + strand, within BWI76_RS03300at 680.923 kb on + strand, within BWI76_RS03300

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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679,369 + BWI76_RS03290 0.36 +0.2
679,507 + BWI76_RS03290 0.51 +0.2
679,546 + BWI76_RS03290 0.55 +0.5
679,657 + BWI76_RS03290 0.67 -0.5
679,657 + BWI76_RS03290 0.67 +0.2
679,880 + +0.1
679,980 + -0.1
679,989 + -0.2
679,998 - -0.3
680,006 + +0.1
680,015 + -1.4
680,020 + +0.1
680,142 + BWI76_RS03295 0.41 -1.3
680,188 - BWI76_RS03295 0.58 +0.8
680,256 + +0.2
680,275 - +0.4
680,327 + +1.3
680,336 - -1.1
680,494 + BWI76_RS03300 0.21 -1.4
680,529 + BWI76_RS03300 0.25 -1.1
680,695 + BWI76_RS03300 0.46 -1.2
680,836 - BWI76_RS03300 0.64 -1.4
680,893 + BWI76_RS03300 0.71 +0.3
680,895 + BWI76_RS03300 0.71 +0.8
680,923 + BWI76_RS03300 0.74 -2.2

Or see this region's nucleotide sequence