Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03165 and BWI76_RS03170 are separated by 17 nucleotides BWI76_RS03170 and BWI76_RS03175 overlap by 4 nucleotides
BWI76_RS03165: BWI76_RS03165 - phage polarity suppression protein, at 654,232 to 654,798
_RS03165
BWI76_RS03170: BWI76_RS03170 - hypothetical protein, at 654,816 to 655,061
_RS03170
BWI76_RS03175: BWI76_RS03175 - hypothetical protein, at 655,058 to 655,795
_RS03175
Position (kb) 654
655
656 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 653.871 kb on + strand at 653.878 kb on + strand at 653.878 kb on + strand at 653.878 kb on - strand at 653.881 kb on + strand at 653.889 kb on - strand at 653.889 kb on - strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.905 kb on - strand at 653.907 kb on + strand at 653.907 kb on + strand at 653.914 kb on + strand at 653.915 kb on - strand at 653.915 kb on - strand at 653.915 kb on - strand at 653.922 kb on - strand at 653.923 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.946 kb on - strand at 653.948 kb on - strand at 653.948 kb on - strand at 653.956 kb on - strand at 653.972 kb on - strand at 653.977 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 654.249 kb on - strand at 654.249 kb on - strand at 654.321 kb on + strand, within BWI76_RS03165 at 654.416 kb on - strand, within BWI76_RS03165 at 654.420 kb on + strand, within BWI76_RS03165 at 654.436 kb on + strand, within BWI76_RS03165 at 654.518 kb on - strand, within BWI76_RS03165 at 654.536 kb on - strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.671 kb on - strand, within BWI76_RS03165 at 654.671 kb on - strand, within BWI76_RS03165 at 654.674 kb on + strand, within BWI76_RS03165 at 654.682 kb on - strand, within BWI76_RS03165 at 654.807 kb on - strand at 654.843 kb on + strand, within BWI76_RS03170 at 654.932 kb on + strand, within BWI76_RS03170 at 654.940 kb on - strand, within BWI76_RS03170 at 654.940 kb on - strand, within BWI76_RS03170 at 655.324 kb on + strand, within BWI76_RS03175 at 655.344 kb on - strand, within BWI76_RS03175 at 655.377 kb on - strand, within BWI76_RS03175 at 655.421 kb on - strand, within BWI76_RS03175 at 655.481 kb on - strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.733 kb on + strand at 656.051 kb on + strand at 656.059 kb on - strand at 656.059 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 653,871 + -0.6 653,878 + -1.8 653,878 + -1.1 653,878 - +0.9 653,881 + +0.1 653,889 - -0.1 653,889 - -0.4 653,892 + +0.3 653,892 + -0.1 653,892 + +1.7 653,892 + -2.0 653,900 - -1.8 653,900 - +0.0 653,900 - -1.1 653,900 - -0.5 653,900 - +0.2 653,900 - -0.2 653,900 - -0.6 653,905 - -0.4 653,907 + +0.2 653,907 + +1.2 653,914 + -1.6 653,915 - +0.6 653,915 - -1.3 653,915 - +0.2 653,922 - +1.3 653,923 + -3.2 653,925 + +1.9 653,925 + +0.3 653,925 + -0.6 653,925 + +4.7 653,925 + +0.2 653,925 + -0.2 653,925 + +0.1 653,925 + -0.7 653,925 + +0.1 653,925 + -2.2 653,925 + +0.2 653,933 - +10.1 653,933 - -0.7 653,933 - +0.3 653,933 - -0.2 653,933 - -0.5 653,933 - +0.8 653,933 - +0.0 653,933 - +0.1 653,933 - -1.2 653,933 - -0.2 653,933 - +0.2 653,933 - -2.1 653,933 - -1.2 653,933 - +0.3 653,943 - +0.6 653,943 - -1.5 653,943 - -0.6 653,943 - +1.2 653,946 - -0.1 653,948 - -0.3 653,948 - -1.1 653,956 - -0.1 653,972 - -0.7 653,977 - -0.7 653,982 - -2.8 653,982 - -0.2 653,982 - +0.8 653,982 - +0.1 654,249 - -0.1 654,249 - -0.4 654,321 + BWI76_RS03165 0.16 -0.4 654,416 - BWI76_RS03165 0.32 +1.5 654,420 + BWI76_RS03165 0.33 -1.1 654,436 + BWI76_RS03165 0.36 -3.3 654,518 - BWI76_RS03165 0.50 +1.3 654,536 - BWI76_RS03165 0.54 -0.9 654,663 + BWI76_RS03165 0.76 -1.7 654,663 + BWI76_RS03165 0.76 -0.6 654,663 + BWI76_RS03165 0.76 -1.0 654,671 - BWI76_RS03165 0.77 +0.4 654,671 - BWI76_RS03165 0.77 -1.4 654,674 + BWI76_RS03165 0.78 +0.2 654,682 - BWI76_RS03165 0.79 +0.4 654,807 - -1.4 654,843 + BWI76_RS03170 0.11 -0.4 654,932 + BWI76_RS03170 0.47 -0.8 654,940 - BWI76_RS03170 0.50 -0.9 654,940 - BWI76_RS03170 0.50 +0.6 655,324 + BWI76_RS03175 0.36 -0.4 655,344 - BWI76_RS03175 0.39 +0.5 655,377 - BWI76_RS03175 0.43 -0.3 655,421 - BWI76_RS03175 0.49 -1.6 655,481 - BWI76_RS03175 0.57 -0.3 655,714 + BWI76_RS03175 0.89 -2.7 655,714 + BWI76_RS03175 0.89 +0.3 655,733 + -1.3 656,051 + -2.0 656,059 - -0.4 656,059 - -0.4
Or see this region's nucleotide sequence