Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02545 and BWI76_RS02550 are separated by 2 nucleotides BWI76_RS02550 and BWI76_RS02555 are separated by 63 nucleotides BWI76_RS02555 and BWI76_RS02560 are separated by 218 nucleotides BWI76_RS02560 and BWI76_RS02565 are separated by 102 nucleotides
BWI76_RS02545: BWI76_RS02545 - protease modulator HflK, at 521,687 to 522,943
_RS02545
BWI76_RS02550: BWI76_RS02550 - protease modulator HflC, at 522,946 to 523,950
_RS02550
BWI76_RS02555: BWI76_RS02555 - hypothetical protein, at 524,014 to 524,304
_RS02555
BWI76_RS02560: BWI76_RS02560 - membrane protein, at 524,523 to 524,720
_RS02560
BWI76_RS02565: BWI76_RS02565 - adenylosuccinate synthase, at 524,823 to 526,121
_RS02565
Position (kb) 522
523
524 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 522.172 kb on - strand, within BWI76_RS02545 at 522.186 kb on - strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.383 kb on - strand, within BWI76_RS02545 at 522.576 kb on - strand, within BWI76_RS02545 at 522.592 kb on + strand, within BWI76_RS02545 at 522.628 kb on + strand, within BWI76_RS02545 at 522.628 kb on + strand, within BWI76_RS02545 at 522.712 kb on + strand, within BWI76_RS02545 at 522.898 kb on - strand at 523.054 kb on + strand, within BWI76_RS02550 at 523.078 kb on + strand, within BWI76_RS02550 at 523.078 kb on + strand, within BWI76_RS02550 at 523.359 kb on - strand, within BWI76_RS02550 at 523.388 kb on + strand, within BWI76_RS02550 at 523.677 kb on - strand, within BWI76_RS02550 at 523.828 kb on + strand, within BWI76_RS02550 at 523.874 kb on + strand at 523.946 kb on + strand at 524.042 kb on + strand at 524.048 kb on + strand, within BWI76_RS02555 at 524.067 kb on + strand, within BWI76_RS02555 at 524.071 kb on + strand, within BWI76_RS02555 at 524.125 kb on - strand, within BWI76_RS02555 at 524.172 kb on + strand, within BWI76_RS02555 at 524.180 kb on - strand, within BWI76_RS02555 at 524.240 kb on + strand at 524.240 kb on + strand, within BWI76_RS02555 at 524.240 kb on + strand, within BWI76_RS02555 at 524.248 kb on - strand, within BWI76_RS02555 at 524.259 kb on - strand, within BWI76_RS02555 at 524.361 kb on - strand at 524.361 kb on - strand at 524.420 kb on + strand at 524.486 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.487 kb on + strand at 524.492 kb on + strand at 524.492 kb on + strand at 524.492 kb on + strand at 524.492 kb on + strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.495 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.500 kb on - strand at 524.555 kb on + strand, within BWI76_RS02560 at 524.555 kb on + strand, within BWI76_RS02560 at 524.556 kb on + strand, within BWI76_RS02560 at 524.564 kb on - strand, within BWI76_RS02560 at 524.580 kb on - strand, within BWI76_RS02560 at 524.585 kb on + strand, within BWI76_RS02560 at 524.721 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 522,172 - BWI76_RS02545 0.39 -5.0 522,186 - BWI76_RS02545 0.40 +1.0 522,375 + BWI76_RS02545 0.55 -1.2 522,375 + BWI76_RS02545 0.55 -1.8 522,375 + BWI76_RS02545 0.55 -0.8 522,383 - BWI76_RS02545 0.55 +0.6 522,576 - BWI76_RS02545 0.71 -0.5 522,592 + BWI76_RS02545 0.72 -2.0 522,628 + BWI76_RS02545 0.75 -0.6 522,628 + BWI76_RS02545 0.75 -0.5 522,712 + BWI76_RS02545 0.82 -0.3 522,898 - +1.6 523,054 + BWI76_RS02550 0.11 -0.8 523,078 + BWI76_RS02550 0.13 -1.6 523,078 + BWI76_RS02550 0.13 -0.4 523,359 - BWI76_RS02550 0.41 -1.7 523,388 + BWI76_RS02550 0.44 -2.5 523,677 - BWI76_RS02550 0.73 -1.2 523,828 + BWI76_RS02550 0.88 -0.5 523,874 + +0.0 523,946 + +0.2 524,042 + -0.8 524,048 + BWI76_RS02555 0.12 -0.6 524,067 + BWI76_RS02555 0.18 -2.4 524,071 + BWI76_RS02555 0.20 -0.1 524,125 - BWI76_RS02555 0.38 -0.5 524,172 + BWI76_RS02555 0.54 +0.8 524,180 - BWI76_RS02555 0.57 +0.3 524,240 + -1.0 524,240 + BWI76_RS02555 0.78 -0.5 524,240 + BWI76_RS02555 0.78 -0.1 524,248 - BWI76_RS02555 0.80 -0.1 524,259 - BWI76_RS02555 0.84 -0.3 524,361 - -2.1 524,361 - -0.3 524,420 + -2.5 524,486 + -1.6 524,487 + -0.1 524,487 + -0.3 524,487 + +0.3 524,487 + -1.2 524,487 + -0.5 524,487 + +0.8 524,487 + -1.2 524,487 + +0.0 524,487 + -0.9 524,487 + +0.3 524,487 + +0.0 524,487 + -1.0 524,487 + -1.6 524,487 + -0.7 524,487 + +1.5 524,487 + +1.1 524,487 + +0.0 524,487 + -1.0 524,487 + +0.3 524,487 + -0.1 524,487 + -0.5 524,487 + -0.6 524,487 + +0.0 524,487 + -0.2 524,487 + +0.9 524,487 + -0.3 524,487 + -0.0 524,492 + -0.2 524,492 + -0.6 524,492 + +1.1 524,492 + +0.3 524,495 - -0.6 524,495 - -0.3 524,495 - +2.2 524,495 - +0.4 524,495 - -0.1 524,495 - +1.2 524,495 - +3.8 524,495 - +0.4 524,495 - +0.6 524,495 - -0.0 524,495 - +1.1 524,495 - +0.1 524,495 - -0.7 524,495 - -0.6 524,495 - +0.4 524,495 - -0.0 524,495 - +0.3 524,495 - -0.4 524,495 - +1.7 524,495 - -2.0 524,495 - +0.2 524,495 - +0.2 524,495 - +0.4 524,495 - +0.9 524,495 - +0.1 524,495 - -0.4 524,495 - -0.6 524,495 - -0.5 524,495 - -0.6 524,495 - +0.2 524,495 - -1.2 524,495 - +1.4 524,500 - +0.2 524,500 - -0.6 524,500 - -0.9 524,500 - +0.0 524,500 - -0.3 524,500 - -2.7 524,500 - +0.2 524,500 - -2.3 524,555 + BWI76_RS02560 0.16 +0.4 524,555 + BWI76_RS02560 0.16 +0.6 524,556 + BWI76_RS02560 0.17 -0.3 524,564 - BWI76_RS02560 0.21 -0.2 524,580 - BWI76_RS02560 0.29 -0.1 524,585 + BWI76_RS02560 0.31 +0.0 524,721 + -0.7
Or see this region's nucleotide sequence