Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01125

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01120 and BWI76_RS01125 overlap by 4 nucleotidesBWI76_RS01125 and BWI76_RS01130 overlap by 4 nucleotides BWI76_RS01120: BWI76_RS01120 - O-antigen translocase, at 230,576 to 231,826 _RS01120 BWI76_RS01125: BWI76_RS01125 - 4-alpha-L-fucosyltransferase, at 231,823 to 232,902 _RS01125 BWI76_RS01130: BWI76_RS01130 - enterobacterial common antigen polymerase, at 232,899 to 234,245 _RS01130 Position (kb) 231 232 233Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 230.926 kb on - strand, within BWI76_RS01120at 230.953 kb on + strand, within BWI76_RS01120at 230.953 kb on + strand, within BWI76_RS01120at 231.208 kb on + strand, within BWI76_RS01120at 231.388 kb on + strand, within BWI76_RS01120at 231.410 kb on + strand, within BWI76_RS01120at 231.627 kb on - strand, within BWI76_RS01120at 231.640 kb on + strand, within BWI76_RS01120at 231.739 kb on - strandat 231.759 kb on - strandat 231.759 kb on - strandat 231.787 kb on + strandat 231.795 kb on - strandat 231.824 kb on + strandat 231.832 kb on - strandat 231.877 kb on + strandat 231.898 kb on + strandat 232.139 kb on - strand, within BWI76_RS01125at 232.219 kb on - strand, within BWI76_RS01125at 232.534 kb on + strand, within BWI76_RS01125at 232.534 kb on + strand, within BWI76_RS01125at 232.534 kb on + strand, within BWI76_RS01125at 232.542 kb on - strand, within BWI76_RS01125at 232.712 kb on - strand, within BWI76_RS01125at 232.727 kb on + strand, within BWI76_RS01125at 232.768 kb on + strand, within BWI76_RS01125at 232.795 kb on - strandat 232.839 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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230,926 - BWI76_RS01120 0.28 -2.7
230,953 + BWI76_RS01120 0.30 -2.5
230,953 + BWI76_RS01120 0.30 -0.8
231,208 + BWI76_RS01120 0.51 -0.7
231,388 + BWI76_RS01120 0.65 -0.3
231,410 + BWI76_RS01120 0.67 -1.1
231,627 - BWI76_RS01120 0.84 +0.4
231,640 + BWI76_RS01120 0.85 -0.9
231,739 - -2.3
231,759 - -3.5
231,759 - -1.1
231,787 + +0.2
231,795 - -1.3
231,824 + +0.4
231,832 - +0.5
231,877 + -2.1
231,898 + -3.4
232,139 - BWI76_RS01125 0.29 +1.0
232,219 - BWI76_RS01125 0.37 -4.5
232,534 + BWI76_RS01125 0.66 -2.2
232,534 + BWI76_RS01125 0.66 -1.1
232,534 + BWI76_RS01125 0.66 +0.1
232,542 - BWI76_RS01125 0.67 -3.4
232,712 - BWI76_RS01125 0.82 +1.0
232,727 + BWI76_RS01125 0.84 -0.2
232,768 + BWI76_RS01125 0.88 -3.6
232,795 - -1.8
232,839 - +0.1

Or see this region's nucleotide sequence