Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01095

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01090 and BWI76_RS01095 overlap by 4 nucleotidesBWI76_RS01095 and BWI76_RS01100 overlap by 4 nucleotides BWI76_RS01090: BWI76_RS01090 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 224,433 to 225,563 _RS01090 BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822 _RS01095 BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887 _RS01100 Position (kb) 225 226 227Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 224.637 kb on - strand, within BWI76_RS01090at 224.826 kb on - strand, within BWI76_RS01090at 224.859 kb on + strand, within BWI76_RS01090at 225.000 kb on - strand, within BWI76_RS01090at 225.010 kb on + strand, within BWI76_RS01090at 225.020 kb on + strand, within BWI76_RS01090at 225.502 kb on + strandat 225.589 kb on + strandat 226.034 kb on - strand, within BWI76_RS01095at 226.533 kb on + strand, within BWI76_RS01095at 226.541 kb on - strand, within BWI76_RS01095at 226.711 kb on + strandat 226.776 kb on + strandat 226.901 kb on + strandat 226.918 kb on + strandat 226.979 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.140 kb on - strand, within BWI76_RS01100at 227.142 kb on + strand, within BWI76_RS01100at 227.227 kb on + strand, within BWI76_RS01100at 227.235 kb on - strand, within BWI76_RS01100at 227.642 kb on - strand, within BWI76_RS01100

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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224,637 - BWI76_RS01090 0.18 +2.1
224,826 - BWI76_RS01090 0.35 -1.0
224,859 + BWI76_RS01090 0.38 -1.4
225,000 - BWI76_RS01090 0.50 -0.1
225,010 + BWI76_RS01090 0.51 +0.5
225,020 + BWI76_RS01090 0.52 -1.3
225,502 + -2.4
225,589 + -2.8
226,034 - BWI76_RS01095 0.38 -0.1
226,533 + BWI76_RS01095 0.77 -2.7
226,541 - BWI76_RS01095 0.78 -2.9
226,711 + -2.4
226,776 + +0.3
226,901 + -1.0
226,918 + -1.6
226,979 + BWI76_RS01100 0.15 -0.9
227,132 + BWI76_RS01100 0.29 -0.7
227,132 + BWI76_RS01100 0.29 +0.3
227,132 + BWI76_RS01100 0.29 +0.1
227,140 - BWI76_RS01100 0.30 -3.2
227,142 + BWI76_RS01100 0.30 -0.9
227,227 + BWI76_RS01100 0.38 -0.2
227,235 - BWI76_RS01100 0.39 -3.2
227,642 - BWI76_RS01100 0.77 -0.6

Or see this region's nucleotide sequence