Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00435 and BWI76_RS00440 are separated by 205 nucleotides BWI76_RS00440 and BWI76_RS00445 overlap by 4 nucleotides BWI76_RS00445 and BWI76_RS00450 are separated by 37 nucleotides BWI76_RS00450 and BWI76_RS00455 are separated by 343 nucleotides BWI76_RS00455 and BWI76_RS00460 overlap by 4 nucleotides
BWI76_RS00435: BWI76_RS00435 - lipase, at 90,170 to 91,087
_RS00435
BWI76_RS00440: BWI76_RS00440 - CopG family transcriptional regulator, at 91,293 to 91,532
_RS00440
BWI76_RS00445: BWI76_RS00445 - PIN domain nuclease, at 91,529 to 91,903
_RS00445
BWI76_RS00450: BWI76_RS00450 - formate dehydrogenase accessory protein FdhE, at 91,941 to 92,870
_RS00450
BWI76_RS00455: BWI76_RS00455 - formate dehydrogenase cytochrome b556 subunit, at 93,214 to 93,849
_RS00455
BWI76_RS00460: BWI76_RS00460 - formate dehydrogenase subunit beta, at 93,846 to 94,748
_RS00460
Position (kb) 91
92
93 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 91.063 kb on + strand at 91.279 kb on + strand at 91.308 kb on + strand at 91.364 kb on + strand, within BWI76_RS00440 at 91.645 kb on - strand, within BWI76_RS00445 at 91.693 kb on + strand, within BWI76_RS00445 at 91.746 kb on + strand, within BWI76_RS00445 at 91.783 kb on + strand, within BWI76_RS00445 at 91.800 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.807 kb on + strand, within BWI76_RS00445 at 91.815 kb on - strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.830 kb on + strand, within BWI76_RS00445 at 91.934 kb on - strand at 91.934 kb on - strand at 91.970 kb on + strand at 91.978 kb on - strand at 92.014 kb on + strand at 92.222 kb on + strand, within BWI76_RS00450 at 92.226 kb on + strand, within BWI76_RS00450 at 92.251 kb on - strand, within BWI76_RS00450 at 92.555 kb on + strand, within BWI76_RS00450 at 92.601 kb on + strand, within BWI76_RS00450 at 92.610 kb on - strand, within BWI76_RS00450 at 92.682 kb on - strand, within BWI76_RS00450 at 92.851 kb on - strand at 93.001 kb on - strand at 93.015 kb on + strand at 93.037 kb on + strand at 93.052 kb on - strand at 93.067 kb on + strand at 93.079 kb on + strand at 93.123 kb on + strand at 93.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 91,063 + +2.3 91,279 + +0.6 91,308 + -0.2 91,364 + BWI76_RS00440 0.30 -0.3 91,645 - BWI76_RS00445 0.31 -0.9 91,693 + BWI76_RS00445 0.44 -0.5 91,746 + BWI76_RS00445 0.58 -0.9 91,783 + BWI76_RS00445 0.68 -0.2 91,800 + BWI76_RS00445 0.72 -0.6 91,807 + BWI76_RS00445 0.74 -0.6 91,807 + BWI76_RS00445 0.74 +0.8 91,807 + BWI76_RS00445 0.74 +0.3 91,815 - BWI76_RS00445 0.76 +0.9 91,830 + BWI76_RS00445 0.80 +2.5 91,830 + BWI76_RS00445 0.80 -1.1 91,830 + BWI76_RS00445 0.80 -0.6 91,934 - -1.3 91,934 - +2.8 91,970 + -2.1 91,978 - +0.6 92,014 + -0.1 92,222 + BWI76_RS00450 0.30 -1.6 92,226 + BWI76_RS00450 0.31 -2.2 92,251 - BWI76_RS00450 0.33 -1.9 92,555 + BWI76_RS00450 0.66 +0.5 92,601 + BWI76_RS00450 0.71 -0.3 92,610 - BWI76_RS00450 0.72 -1.2 92,682 - BWI76_RS00450 0.80 +0.5 92,851 - +0.5 93,001 - -0.4 93,015 + -0.7 93,037 + -2.2 93,052 - -0.4 93,067 + -1.2 93,079 + -1.2 93,123 + -0.0 93,135 - -2.4
Or see this region's nucleotide sequence