Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00325 and BWI76_RS00330 overlap by 4 nucleotides BWI76_RS00330 and BWI76_RS00335 are separated by 71 nucleotides BWI76_RS00335 and BWI76_RS00340 are separated by 75 nucleotides BWI76_RS00340 and BWI76_RS00345 are separated by 27 nucleotides BWI76_RS00345 and BWI76_RS00350 are separated by 49 nucleotides
BWI76_RS00325: BWI76_RS00325 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 69,712 to 70,218
_RS00325
BWI76_RS00330: BWI76_RS00330 - molybdenum cofactor guanylyltransferase, at 70,215 to 70,796
_RS00330
BWI76_RS00335: BWI76_RS00335 - hypothetical protein, at 70,868 to 71,137
_RS00335
BWI76_RS00340: BWI76_RS00340 - stress response kinase A, at 71,213 to 72,199
_RS00340
BWI76_RS00345: BWI76_RS00345 - protein disulfide oxidoreductase DsbA, at 72,227 to 72,850
_RS00345
BWI76_RS00350: BWI76_RS00350 - acyltransferase, at 72,900 to 73,799
_RS00350
Position (kb) 71
72
73 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 70.364 kb on - strand, within BWI76_RS00330 at 70.386 kb on + strand, within BWI76_RS00330 at 70.525 kb on + strand, within BWI76_RS00330 at 70.791 kb on - strand at 70.829 kb on - strand at 70.835 kb on + strand at 70.844 kb on - strand at 70.913 kb on + strand, within BWI76_RS00335 at 71.051 kb on - strand, within BWI76_RS00335 at 71.115 kb on - strand at 71.188 kb on + strand at 71.224 kb on + strand at 71.232 kb on - strand at 71.303 kb on - strand at 71.317 kb on - strand, within BWI76_RS00340 at 71.529 kb on + strand, within BWI76_RS00340 at 71.637 kb on - strand, within BWI76_RS00340 at 72.120 kb on + strand at 72.151 kb on + strand at 72.151 kb on + strand at 72.151 kb on + strand at 72.151 kb on + strand at 72.151 kb on + strand at 72.158 kb on + strand at 72.159 kb on - strand at 72.159 kb on - strand at 72.159 kb on - strand at 72.159 kb on - strand at 72.159 kb on - strand at 72.201 kb on - strand at 72.307 kb on + strand, within BWI76_RS00345 at 72.339 kb on + strand, within BWI76_RS00345 at 72.510 kb on - strand, within BWI76_RS00345 at 72.510 kb on - strand, within BWI76_RS00345 at 72.592 kb on - strand, within BWI76_RS00345 at 72.592 kb on - strand, within BWI76_RS00345 at 72.661 kb on - strand, within BWI76_RS00345 at 72.745 kb on - strand, within BWI76_RS00345 at 72.822 kb on + strand at 72.830 kb on - strand at 72.832 kb on - strand at 72.875 kb on + strand at 72.875 kb on + strand at 72.875 kb on + strand at 72.883 kb on - strand at 72.883 kb on - strand at 72.883 kb on - strand at 72.959 kb on + strand at 72.959 kb on + strand at 72.967 kb on - strand at 73.004 kb on + strand, within BWI76_RS00350 at 73.019 kb on + strand, within BWI76_RS00350 at 73.047 kb on + strand, within BWI76_RS00350 at 73.047 kb on - strand, within BWI76_RS00350 at 73.111 kb on + strand, within BWI76_RS00350 at 73.111 kb on + strand, within BWI76_RS00350 at 73.111 kb on + strand, within BWI76_RS00350 at 73.111 kb on + strand, within BWI76_RS00350 at 73.121 kb on + strand, within BWI76_RS00350 at 73.129 kb on - strand, within BWI76_RS00350 at 73.129 kb on - strand, within BWI76_RS00350 at 73.129 kb on - strand, within BWI76_RS00350 at 73.136 kb on + strand, within BWI76_RS00350 at 73.152 kb on + strand, within BWI76_RS00350 at 73.169 kb on - strand, within BWI76_RS00350
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 70,364 - BWI76_RS00330 0.26 -1.3 70,386 + BWI76_RS00330 0.29 -0.4 70,525 + BWI76_RS00330 0.53 -0.8 70,791 - -0.7 70,829 - -0.3 70,835 + -0.1 70,844 - -0.9 70,913 + BWI76_RS00335 0.17 +0.5 71,051 - BWI76_RS00335 0.68 -0.3 71,115 - -1.2 71,188 + -0.5 71,224 + +0.5 71,232 - -0.1 71,303 - -2.0 71,317 - BWI76_RS00340 0.11 +1.1 71,529 + BWI76_RS00340 0.32 +0.5 71,637 - BWI76_RS00340 0.43 -0.0 72,120 + +0.5 72,151 + +1.4 72,151 + -0.9 72,151 + +0.4 72,151 + -0.6 72,151 + +2.3 72,158 + -0.7 72,159 - -1.4 72,159 - -0.9 72,159 - +0.1 72,159 - -0.0 72,159 - +1.3 72,201 - -0.4 72,307 + BWI76_RS00345 0.13 -2.4 72,339 + BWI76_RS00345 0.18 +1.0 72,510 - BWI76_RS00345 0.45 -0.5 72,510 - BWI76_RS00345 0.45 -0.2 72,592 - BWI76_RS00345 0.58 +1.5 72,592 - BWI76_RS00345 0.58 +0.5 72,661 - BWI76_RS00345 0.70 -1.5 72,745 - BWI76_RS00345 0.83 +2.3 72,822 + -1.8 72,830 - +0.7 72,832 - +1.0 72,875 + +0.3 72,875 + +1.0 72,875 + -0.2 72,883 - +4.9 72,883 - +0.6 72,883 - +1.5 72,959 + +1.9 72,959 + -2.0 72,967 - -1.6 73,004 + BWI76_RS00350 0.12 +0.0 73,019 + BWI76_RS00350 0.13 +0.2 73,047 + BWI76_RS00350 0.16 +0.3 73,047 - BWI76_RS00350 0.16 -1.3 73,111 + BWI76_RS00350 0.23 -0.3 73,111 + BWI76_RS00350 0.23 -1.1 73,111 + BWI76_RS00350 0.23 -2.5 73,111 + BWI76_RS00350 0.23 +0.0 73,121 + BWI76_RS00350 0.25 -2.5 73,129 - BWI76_RS00350 0.25 -1.0 73,129 - BWI76_RS00350 0.25 +0.1 73,129 - BWI76_RS00350 0.25 -1.3 73,136 + BWI76_RS00350 0.26 -1.0 73,152 + BWI76_RS00350 0.28 +3.6 73,169 - BWI76_RS00350 0.30 -0.7
Or see this region's nucleotide sequence