Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00085

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotidesBWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotidesBWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotides BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709 _RS00075 BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581 _RS00080 BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042 _RS00085 BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393 _RS00090 Position (kb) 16 17 18Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 16.009 kb on + strand, within BWI76_RS00080at 16.225 kb on + strand, within BWI76_RS00080at 16.451 kb on + strand, within BWI76_RS00080at 16.461 kb on + strand, within BWI76_RS00080at 16.469 kb on - strand, within BWI76_RS00080at 16.514 kb on - strandat 16.529 kb on - strandat 16.601 kb on - strandat 16.609 kb on + strandat 16.616 kb on + strandat 16.631 kb on + strand, within BWI76_RS00085at 16.631 kb on + strand, within BWI76_RS00085at 16.932 kb on - strand, within BWI76_RS00085at 17.020 kb on - strandat 17.088 kb on - strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.184 kb on - strandat 17.184 kb on - strandat 17.214 kb on - strandat 17.244 kb on + strandat 17.252 kb on - strandat 17.252 kb on - strandat 17.498 kb on - strand, within BWI76_RS00090at 17.539 kb on + strand, within BWI76_RS00090at 17.546 kb on + strand, within BWI76_RS00090at 17.672 kb on + strand, within BWI76_RS00090at 17.683 kb on + strand, within BWI76_RS00090at 17.688 kb on + strand, within BWI76_RS00090at 17.696 kb on - strand, within BWI76_RS00090at 17.881 kb on - strand, within BWI76_RS00090at 17.963 kb on + strand, within BWI76_RS00090at 18.025 kb on - strand, within BWI76_RS00090

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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16,009 + BWI76_RS00080 0.35 +0.1
16,225 + BWI76_RS00080 0.59 +0.8
16,451 + BWI76_RS00080 0.85 -0.7
16,461 + BWI76_RS00080 0.86 +0.3
16,469 - BWI76_RS00080 0.87 +0.9
16,514 - -0.4
16,529 - -1.0
16,601 - +0.1
16,609 + -0.3
16,616 + -3.3
16,631 + BWI76_RS00085 0.11 +0.0
16,631 + BWI76_RS00085 0.11 -0.3
16,932 - BWI76_RS00085 0.76 -0.4
17,020 - -1.0
17,088 - +0.1
17,176 + -0.3
17,176 + +0.1
17,176 + -1.0
17,176 + -0.0
17,184 - +2.9
17,184 - -0.1
17,214 - -0.2
17,244 + +0.2
17,252 - -0.9
17,252 - -0.0
17,498 - BWI76_RS00090 0.24 -2.3
17,539 + BWI76_RS00090 0.28 +0.1
17,546 + BWI76_RS00090 0.28 +0.5
17,672 + BWI76_RS00090 0.39 -3.4
17,683 + BWI76_RS00090 0.40 +0.9
17,688 + BWI76_RS00090 0.40 +0.5
17,696 - BWI76_RS00090 0.41 -1.4
17,881 - BWI76_RS00090 0.57 -0.6
17,963 + BWI76_RS00090 0.64 +5.6
18,025 - BWI76_RS00090 0.69 +0.5

Or see this region's nucleotide sequence