Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27075

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27065 and BWI76_RS27070 are separated by 101 nucleotidesBWI76_RS27070 and BWI76_RS27075 are separated by 118 nucleotidesBWI76_RS27075 and BWI76_RS27080 are separated by 217 nucleotides BWI76_RS27065: BWI76_RS27065 - valine--pyruvate transaminase, at 5,616,666 to 5,617,925 _RS27065 BWI76_RS27070: BWI76_RS27070 - 4Fe-4S ferredoxin, at 5,618,027 to 5,618,515 _RS27070 BWI76_RS27075: BWI76_RS27075 - IclR family transcriptional regulator, at 5,618,634 to 5,619,452 _RS27075 BWI76_RS27080: BWI76_RS27080 - 3-dehydro-L-gulonate 2-dehydrogenase, at 5,619,670 to 5,620,668 _RS27080 Position (kb) 5618 5619 5620Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 5617.696 kb on + strand, within BWI76_RS27065at 5617.697 kb on + strand, within BWI76_RS27065at 5617.705 kb on - strand, within BWI76_RS27065at 5617.763 kb on - strand, within BWI76_RS27065at 5617.903 kb on + strandat 5617.913 kb on + strandat 5617.969 kb on - strandat 5618.091 kb on + strand, within BWI76_RS27070at 5618.104 kb on - strandat 5618.185 kb on + strand, within BWI76_RS27070at 5618.224 kb on + strand, within BWI76_RS27070at 5618.234 kb on - strand, within BWI76_RS27070at 5618.255 kb on + strand, within BWI76_RS27070at 5618.413 kb on + strand, within BWI76_RS27070at 5618.413 kb on + strand, within BWI76_RS27070at 5618.421 kb on - strand, within BWI76_RS27070at 5618.423 kb on + strand, within BWI76_RS27070at 5618.431 kb on - strand, within BWI76_RS27070at 5618.431 kb on - strand, within BWI76_RS27070at 5618.431 kb on - strand, within BWI76_RS27070at 5618.638 kb on + strandat 5618.800 kb on + strand, within BWI76_RS27075at 5618.906 kb on - strand, within BWI76_RS27075at 5618.995 kb on + strand, within BWI76_RS27075at 5619.003 kb on + strand, within BWI76_RS27075at 5619.100 kb on - strand, within BWI76_RS27075at 5619.196 kb on - strand, within BWI76_RS27075at 5619.264 kb on + strand, within BWI76_RS27075at 5619.392 kb on + strandat 5619.790 kb on - strand, within BWI76_RS27080at 5619.793 kb on + strand, within BWI76_RS27080at 5619.837 kb on + strand, within BWI76_RS27080at 5619.974 kb on + strand, within BWI76_RS27080at 5619.990 kb on - strand, within BWI76_RS27080at 5619.991 kb on - strand, within BWI76_RS27080at 5620.042 kb on - strand, within BWI76_RS27080at 5620.112 kb on + strand, within BWI76_RS27080at 5620.215 kb on + strand, within BWI76_RS27080at 5620.223 kb on - strand, within BWI76_RS27080at 5620.326 kb on + strand, within BWI76_RS27080at 5620.337 kb on + strand, within BWI76_RS27080at 5620.386 kb on + strand, within BWI76_RS27080at 5620.389 kb on + strand, within BWI76_RS27080

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
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5,617,696 + BWI76_RS27065 0.82 +0.8
5,617,697 + BWI76_RS27065 0.82 +0.3
5,617,705 - BWI76_RS27065 0.82 -0.2
5,617,763 - BWI76_RS27065 0.87 +1.4
5,617,903 + +0.6
5,617,913 + +0.2
5,617,969 - -0.1
5,618,091 + BWI76_RS27070 0.13 +0.1
5,618,104 - +2.0
5,618,185 + BWI76_RS27070 0.32 +1.4
5,618,224 + BWI76_RS27070 0.40 +0.1
5,618,234 - BWI76_RS27070 0.42 -0.4
5,618,255 + BWI76_RS27070 0.47 +0.3
5,618,413 + BWI76_RS27070 0.79 -0.1
5,618,413 + BWI76_RS27070 0.79 +0.4
5,618,421 - BWI76_RS27070 0.81 -1.0
5,618,423 + BWI76_RS27070 0.81 +0.9
5,618,431 - BWI76_RS27070 0.83 +1.4
5,618,431 - BWI76_RS27070 0.83 +0.5
5,618,431 - BWI76_RS27070 0.83 -1.0
5,618,638 + -1.4
5,618,800 + BWI76_RS27075 0.20 -1.8
5,618,906 - BWI76_RS27075 0.33 -0.0
5,618,995 + BWI76_RS27075 0.44 -0.5
5,619,003 + BWI76_RS27075 0.45 -3.8
5,619,100 - BWI76_RS27075 0.57 -2.0
5,619,196 - BWI76_RS27075 0.69 -1.8
5,619,264 + BWI76_RS27075 0.77 +0.6
5,619,392 + -1.9
5,619,790 - BWI76_RS27080 0.12 -0.1
5,619,793 + BWI76_RS27080 0.12 -1.1
5,619,837 + BWI76_RS27080 0.17 +0.6
5,619,974 + BWI76_RS27080 0.30 +4.8
5,619,990 - BWI76_RS27080 0.32 +0.2
5,619,991 - BWI76_RS27080 0.32 +4.1
5,620,042 - BWI76_RS27080 0.37 -0.5
5,620,112 + BWI76_RS27080 0.44 -0.6
5,620,215 + BWI76_RS27080 0.55 +0.5
5,620,223 - BWI76_RS27080 0.55 -0.7
5,620,326 + BWI76_RS27080 0.66 -0.2
5,620,337 + BWI76_RS27080 0.67 -1.4
5,620,386 + BWI76_RS27080 0.72 +0.4
5,620,389 + BWI76_RS27080 0.72 +0.1

Or see this region's nucleotide sequence