Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27000 and BWI76_RS27005 are separated by 127 nucleotides BWI76_RS27005 and BWI76_RS27010 are separated by 180 nucleotides BWI76_RS27010 and BWI76_RS27015 overlap by 4 nucleotides BWI76_RS27015 and BWI76_RS27020 are separated by 168 nucleotides
BWI76_RS27000: BWI76_RS27000 - glycine--tRNA ligase subunit alpha, at 5,601,904 to 5,602,815
_RS27000
BWI76_RS27005: BWI76_RS27005 - hypothetical protein, at 5,602,943 to 5,603,248
_RS27005
BWI76_RS27010: BWI76_RS27010 - O-acetyltransferase, at 5,603,429 to 5,604,424
_RS27010
BWI76_RS27015: BWI76_RS27015 - hypothetical protein, at 5,604,421 to 5,604,801
_RS27015
BWI76_RS27020: BWI76_RS27020 - Xylulose kinase (EC 2.7.1.17) <small>(from data)</small>, at 5,604,970 to 5,606,424
_RS27020
Position (kb) 5603
5604
5605 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5603.077 kb on + strand, within BWI76_RS27005 at 5603.080 kb on - strand, within BWI76_RS27005 at 5603.085 kb on - strand, within BWI76_RS27005 at 5603.100 kb on + strand, within BWI76_RS27005 at 5603.183 kb on - strand, within BWI76_RS27005 at 5603.191 kb on + strand, within BWI76_RS27005 at 5603.400 kb on - strand at 5603.492 kb on + strand at 5603.529 kb on + strand, within BWI76_RS27010 at 5603.537 kb on - strand, within BWI76_RS27010 at 5603.701 kb on + strand, within BWI76_RS27010 at 5603.701 kb on + strand, within BWI76_RS27010 at 5603.725 kb on + strand, within BWI76_RS27010 at 5603.725 kb on + strand, within BWI76_RS27010 at 5603.733 kb on - strand, within BWI76_RS27010 at 5603.733 kb on - strand, within BWI76_RS27010 at 5603.835 kb on - strand, within BWI76_RS27010 at 5603.896 kb on + strand, within BWI76_RS27010 at 5603.904 kb on - strand, within BWI76_RS27010 at 5603.969 kb on - strand, within BWI76_RS27010 at 5603.990 kb on + strand, within BWI76_RS27010 at 5603.998 kb on - strand, within BWI76_RS27010 at 5604.043 kb on + strand, within BWI76_RS27010 at 5604.053 kb on + strand, within BWI76_RS27010 at 5604.056 kb on - strand, within BWI76_RS27010 at 5604.061 kb on - strand, within BWI76_RS27010 at 5604.061 kb on - strand, within BWI76_RS27010 at 5604.061 kb on - strand, within BWI76_RS27010 at 5604.203 kb on - strand at 5604.210 kb on + strand, within BWI76_RS27010 at 5604.364 kb on - strand at 5604.570 kb on + strand, within BWI76_RS27015 at 5604.594 kb on + strand, within BWI76_RS27015 at 5604.684 kb on + strand, within BWI76_RS27015 at 5604.759 kb on + strand, within BWI76_RS27015 at 5604.814 kb on + strand at 5604.814 kb on + strand at 5604.814 kb on + strand at 5604.814 kb on + strand at 5604.822 kb on - strand at 5604.822 kb on - strand at 5604.822 kb on - strand at 5604.822 kb on - strand at 5604.822 kb on - strand at 5604.893 kb on + strand at 5605.154 kb on + strand, within BWI76_RS27020 at 5605.330 kb on - strand, within BWI76_RS27020 at 5605.402 kb on + strand, within BWI76_RS27020
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,603,077 + BWI76_RS27005 0.44 -0.6 5,603,080 - BWI76_RS27005 0.45 -0.3 5,603,085 - BWI76_RS27005 0.46 -1.8 5,603,100 + BWI76_RS27005 0.51 -0.2 5,603,183 - BWI76_RS27005 0.78 -0.0 5,603,191 + BWI76_RS27005 0.81 +1.5 5,603,400 - -0.9 5,603,492 + +0.1 5,603,529 + BWI76_RS27010 0.10 -0.2 5,603,537 - BWI76_RS27010 0.11 -0.3 5,603,701 + BWI76_RS27010 0.27 -1.9 5,603,701 + BWI76_RS27010 0.27 -1.2 5,603,725 + BWI76_RS27010 0.30 -0.3 5,603,725 + BWI76_RS27010 0.30 -0.1 5,603,733 - BWI76_RS27010 0.31 +0.6 5,603,733 - BWI76_RS27010 0.31 -1.3 5,603,835 - BWI76_RS27010 0.41 -0.1 5,603,896 + BWI76_RS27010 0.47 +1.0 5,603,904 - BWI76_RS27010 0.48 +1.5 5,603,969 - BWI76_RS27010 0.54 +0.9 5,603,990 + BWI76_RS27010 0.56 +0.0 5,603,998 - BWI76_RS27010 0.57 +1.2 5,604,043 + BWI76_RS27010 0.62 +1.5 5,604,053 + BWI76_RS27010 0.63 -0.1 5,604,056 - BWI76_RS27010 0.63 +1.6 5,604,061 - BWI76_RS27010 0.63 -0.8 5,604,061 - BWI76_RS27010 0.63 -0.5 5,604,061 - BWI76_RS27010 0.63 -0.1 5,604,203 - -0.5 5,604,210 + BWI76_RS27010 0.78 +2.6 5,604,364 - +0.1 5,604,570 + BWI76_RS27015 0.39 +0.2 5,604,594 + BWI76_RS27015 0.45 +0.4 5,604,684 + BWI76_RS27015 0.69 -0.7 5,604,759 + BWI76_RS27015 0.89 -0.3 5,604,814 + +0.9 5,604,814 + +0.2 5,604,814 + -1.1 5,604,814 + -0.4 5,604,822 - +0.8 5,604,822 - +0.9 5,604,822 - -0.5 5,604,822 - +0.4 5,604,822 - +0.6 5,604,893 + -0.6 5,605,154 + BWI76_RS27020 0.13 -0.2 5,605,330 - BWI76_RS27020 0.25 -0.1 5,605,402 + BWI76_RS27020 0.30 -0.4
Or see this region's nucleotide sequence