Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24265 and BWI76_RS24270 are separated by 121 nucleotides BWI76_RS24270 and BWI76_RS24275 are separated by 142 nucleotides BWI76_RS24275 and BWI76_RS24280 are separated by 6 nucleotides BWI76_RS24280 and BWI76_RS24285 are separated by 251 nucleotides
BWI76_RS24265: BWI76_RS24265 - dienelactone hydrolase, at 5,056,261 to 5,057,148
_RS24265
BWI76_RS24270: BWI76_RS24270 - membrane protein, at 5,057,270 to 5,057,764
_RS24270
BWI76_RS24275: BWI76_RS24275 - biopolymer transport protein ExbD, at 5,057,907 to 5,058,332
_RS24275
BWI76_RS24280: BWI76_RS24280 - biopolymer transporter ExbB, at 5,058,339 to 5,059,070
_RS24280
BWI76_RS24285: BWI76_RS24285 - cystathionine beta-lyase, at 5,059,322 to 5,060,509
_RS24285
Position (kb) 5057
5058
5059 Strain fitness (log2 ratio) -2
-1
0
1 at 5056.965 kb on + strand, within BWI76_RS24265 at 5057.008 kb on - strand, within BWI76_RS24265 at 5057.306 kb on - strand at 5057.357 kb on - strand, within BWI76_RS24270 at 5057.554 kb on + strand, within BWI76_RS24270 at 5057.598 kb on + strand, within BWI76_RS24270 at 5057.690 kb on - strand, within BWI76_RS24270 at 5057.794 kb on + strand at 5057.794 kb on + strand at 5057.872 kb on + strand at 5057.879 kb on - strand at 5057.974 kb on + strand, within BWI76_RS24275 at 5057.988 kb on - strand, within BWI76_RS24275 at 5058.135 kb on - strand, within BWI76_RS24275 at 5058.382 kb on - strand at 5058.598 kb on - strand, within BWI76_RS24280 at 5058.746 kb on + strand, within BWI76_RS24280 at 5058.805 kb on - strand, within BWI76_RS24280 at 5058.990 kb on + strand, within BWI76_RS24280 at 5059.184 kb on + strand at 5059.256 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 5,056,965 + BWI76_RS24265 0.79 -0.5 5,057,008 - BWI76_RS24265 0.84 +0.3 5,057,306 - -0.4 5,057,357 - BWI76_RS24270 0.18 -1.0 5,057,554 + BWI76_RS24270 0.57 +1.1 5,057,598 + BWI76_RS24270 0.66 -0.6 5,057,690 - BWI76_RS24270 0.85 +0.4 5,057,794 + -0.0 5,057,794 + +0.2 5,057,872 + -0.4 5,057,879 - +0.2 5,057,974 + BWI76_RS24275 0.16 -1.9 5,057,988 - BWI76_RS24275 0.19 -0.8 5,058,135 - BWI76_RS24275 0.54 -0.8 5,058,382 - +0.1 5,058,598 - BWI76_RS24280 0.35 -1.2 5,058,746 + BWI76_RS24280 0.56 -1.1 5,058,805 - BWI76_RS24280 0.64 -1.4 5,058,990 + BWI76_RS24280 0.89 -1.5 5,059,184 + +0.1 5,059,256 - +0.3
Or see this region's nucleotide sequence